50 results about "Pulmonary aspergillosis" patented technology

Formulations are provided for pulmonary administration of an antifungal agent to a patient. Methods of using the formulations in the treatment of antifungal infections are also provided, including treatment of pulmonary aspergillosis with amphotericin B-containing formulations. Methods of manufacturing the formulations to achieve optimum properties are provided as well.

The invention relates to a freeze-drying composition of a posaconazole prodrug and a preparation method and application of the freeze-drying composition of the posaconazole prodrug. The freeze-drying composition has the advantages that the freeze-drying composition is high in water solubility, and safety of the freeze-drying composition is guaranteed due to the fact that cyclodextrins auxiliary materials need not to be added during the preparation of the freeze-drying composition; the freeze-drying composition is suitable for being used for treating various amphotericin-intolerant or refractory adult invasive fungal infections; the freeze-drying composition is used as a preventive drug for high-risk patients, the freeze-drying composition is applicable to patients above 13 years old and with impaired immunity and especially applicable to patients who have graft versus host disease (GVHD) after hematopoietic stem cell transplant, patients with leukemia and patients with long-term leukopenia due to chemotherapy; compared with control drugs such as fluconazole and itraconazole, the freeze-drying composition can effectively prevent invasive aspergillosis and can lower the mortality related to the invasive fungal infections.

The invention discloses an Aspergillus flavus avirulent strain and an application of the Aspergillus flavus avirulent strain in controlling pollution of Aspergillus flavus. In the avirulent strain, the Aspergillus flavus does not express pathogenesis related genes aflash1. The strain is non-toxic, does not produce sclerotium, produces a large quantity of spores in a culture medium and a small quantity of spores on an organism and has low pathogenicity. On the basis of the characteristics of the strain, the invention further discloses a method for controlling pollution of the Aspergillus flavus by use of the strain. More specifically, the method comprises steps as follows: by use of the characteristics that the Aspergillus flavus strain is non-toxic, does not produce sclerotium, produces a large quantity of spores in a culture medium and a small quantity of spores on an organism and has low pathogenicity, limited ecological niches are scrambled in main peanut producing areas and other susceptible areas of Aspergillus flavus through the strain and wild toxic-producing Aspergillus flavus strains, the density of the wild toxic-producing Aspergillus flavus strains is effectively reduced, and Aspergillus flavus infection to crops, Aspergillus flavus toxin pollution and aspergillosis infection caused by Aspergillus flavus toxin pollution are effectively controlled and reduced.

The combination of pentraxin PTX3 with antifungal agents is described for the treatment of fungal infections and particularly for infections caused by Aspergillus fumigatus. Thanks to the synergistic effect of the two drugs, the combination allows the administration of suboptimal doses of antifungal agent.

This application relates to the production and characterization of a mutant strain of Aspergillus fumigatus. The application also relates to a method for inhibiting siderophore biosynthesis in Aspergillus fumigatus and an assay for identifying drug candidates or other agents having potential inhibitory activity. The method may comprise, for example, the step of inhibiting an enzyme catalyzing siderophore biosynthesis, such as L-ornithine N⁵-oxygenase. In one embodiment the siderophore is a hydroxamate siderophore, such as N′N″N′″-triacetylfusarinine C (TAF) or ferricrocin. A method of preventing or treating fungal infections in a patient is also described comprising administering to the patient an agent suitable for inhibiting fungal secretion of siderophores. The method is particularly useful for immunocompromised patients susceptible to fungal infections caused by Aspergillus fumigatus, such as pulmonary aspergillosis. The invention also relates to diagnostic methods for detecting a biomarker indicative of likely A fumigatus infection in vivo, such as serum presence of TAF.

The invention discloses Saccharomyces ellipsoideus BY36, with broad bacteriostasis spectrum and stable effect, for preventing and treating postharvest diseases of fruits and vegetables and a use method and use thereof. The strain has a collection strain serial number of CGMCC No. 14910 in General Microorganisms Center of China Committee for Culture Collection of Microorganisms. The use method of the Saccharomyces ellipsoideus comprises the steps: activating the strain, carrying out fermentation culture with YPD, carrying out centrifugation, and preparing a 1*10<8>cell/mL bacterial suspension from thalli and sterile water; placing the fruits and vegetables such as apples, pears, grapes, strawberries, oranges or cherry tomatoes into the bacterial suspension, carrying out soaking for 30 seconds, then, taking out the fruits and vegetables, and drying the fruits and vegetables in the air; and placing the fruits and vegetables into a fresh-keeping box, and carrying out storage at normal temperature. The Saccharomyces ellipsoideus strain can be used for simultaneously controlling blue molds and gray molds of apples, gray molds of pears, gray molds, aspergillosis, black spots, anthrax andpink rot of grapes, gray molds of strawberries, blue molds of oranges and gray molds and aspergillosis of cherry tomatoes, and loss caused by the postharvest diseases is reduced, so that the Saccharomyces ellipsoideus strain has a very good application prospect.

Oligonucleotides and methods for using these oligonucleotides in the detection of Aspergillus fumigatus are disclosed. Aspergillus fumigatus is the causative agent for medical conditions including invasive aspergillosis. The oligonucleotides of the invention have nucleotide sequences derived from the gene encoding the cytochrome P450 14 alpha-sterol demethylase (i.e., the Cyp51A protein) of Aspergillus fumigatus. The oligonucleotides of the invention include forward primers and reverse primers which in combination are capable of priming the synthesis of amplicons specific to cyp51A in polymerase chain reactions using nucleic acids isolated from Aspergillus fumigatus as templates. The oligonucleotides of the invention also include probes capable of detecting these cyp51A-specific amplicons. Thus, a biological sample is tested for the presence of Aspergillus fumigatus by isolating nucleic acid from the sample, conducting a polymerase chain reaction in a mixture containing this nucleic acid and these forward and reverse primers, and then determining, using an oligonucleotide probe, whether an amplicon is produced in the mixture, wherein detection of the amplicon indicates the presence of Aspergillus fumigatus in the sample. The oligonucleotides of the invention also include primers for nucleotide sequencing reactions to determine whether an isolate of Aspergillus fumigatus is more tolerant than wild-type Aspergillus fumigatus to a triazole, which is a compound commonly used as an antifungal drug. Specifically, a strand of a cyp51A-specific amplicon is at least partially sequenced using a nucleotide sequencing primer in a primer extension reaction. Identification of mutations giving rise to amino acid substitutions at positions 54, 138, 220, and 448 of the amino acid sequence of the wild-type Cyp51A protein indicates that the isolate of Aspergillus fumigatus from which the amplicon is derived exhibits decreased susceptibility to at least one triazole.

The invention discloses a hanseniaspora vineae BY19 for preventing and controlling postharvest diseases of fruits and vegetables and with wide antibacterial spectrum and stable effect and a use methodthereof. The collection number of the strain in China General Microbiological Culture Collection Center is CGMCC No. 14903. The use method of the hanseniaspora vineae comprises the following steps of: activating the strain, carrying out fermentation culture by using YPD, centrifuging, and preparing thalli into 1*108 cell/mL of bacterial suspension by using sterile water; putting fruits and vegetables such as apples, pears, grapes, strawberries, citruses or cherry tomatoes and the like into the bacterial suspension, soaking for 30 seconds, then taking out, and carrying out air drying; puttinginto a fresh-keeping box, and storing at normal temperature. The hanseniaspora vineae strain disclosed by the invention has the beneficial effects that penicilliosis and botrytis cinerea of the apples, the penicilliosis and the botrytis cinerea of the pears, botrytis, aspergillosis, black spot, fusaridiosis and anthracnose of the grapes, the botrytis of the strawberries and the botrytis and the aspergillosis of the cherry tomatoes can be controlled simultaneously, so that the loss caused by the postharvest diseases is reduced and the application prospect is good.

The present invention discloses a medicine which is used for preventing and treating chicken aspergillosis. The ingredients have the following weight portions: 5 to 20 portions of lance asiabell root, 5 to 20 portions of cordate houttuynia, 5 to 20 portions of dandelion, 5 to 20 portions of ajuga ciliata, 5 to 20 portions of radix scutellariae, 3 to 12 portions of balloon-flower root, 3 to 12 portions of fried Chinese radish seed, 3 to 12 portions of fried draba nemorosa linnei, 3 to 12 portions of fried perilla, 3 to 12 portions of radix sophorae, 2 to 6 portions of pipewort, 2 to 6 portions of sicklesenna seed, 2 to 6 portions of Psoraleae, 2 to 6 portions of bark of pomegranate, 2 to 6 portions of ebony, 2 to 6 portions of rhubarb, 2 to 6 portions of lespedeza, and 2 to 6 portions of licorice. The herbs are insolated, weighed, crushed, filtered by a sieve with 60 to 80 meshes, and mixed well, so as to prepare the medicine. When in clinical use, the medicine is dipped for 30 to 60 minutes in boiling water, so that the effective ingredients can be fully dissolved and separated to facilitate the absorption in the body of the sick chicken. Simultaneously, a small amount of sugar and Vc are added to improve the medicinal effects. The medicine has high cure rate, no adverse reaction, no drug residues in the body, and nearly no drug resistance. The production of the medicine has the advantages of easily accessible raw materials, simple preparation, convenient use, and capability of wide promotion and application in the chicken breeding industry.

The invention discloses a metschnikowia fructicola strain BY33, which is wide in antibacterial spectrum and stable in effects, for preventing and controlling postharvest diseases of fruits and vegetables, and a using method and use of the metschnikowia fructicola strain BY33. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC No. 14908. The using method of the metschnikowia fructicola comprises the following steps: activating a strain, carrying out fermentation culture on the strain with YPD, centrifuging, and preparing thallus into 1*10<8> cells/mL bacterial suspension with sterile water; putting fruits and vegetables such as apples, pears, grapes, strawberries, citrus or cherry tomatoes into the bacterial suspension, soaking for 30s and then taking out, and drying in the air; putting the dried products into preservation boxes, and storing at room temperature. The metschnikowia fructicola strain can be used for controlling apple penicillium, gray mold and black spot, pear fruit penicillium and gray mold, grape gray mold, aspergillosis, fusarium mildew and anthracnose, strawberry gray mold and aspergillosis, citrus penicillium as well as cherry tomato gray mold and aspergillosis at the same time, and reduces the loss caused by the postharvest diseases, thus having a very good application prospect.

The invention relates to the technical field of poultry forages, and especially relates to a feed additive for preventing chicken aspergillosis. The additive comprises, in parts by mass, 10-15 parts of houttuynia cordata powder, 10-15 parts of dandelion powder, 2-5 parts of algae powder, 2-5 parts of sea-tangle powder, 1-4 parts of powder of a sea plant named Haigucai, 0.2-0.4 part of anti-oxidizing quinoline and 0.3-0.5 part of compound vitamin. By employing the technical scheme, the beneficial effects comprise that the additive disclosed by the invention is capable of prolonging the storage time of forages and preventing forages to mildew, thereby reducing generation probability of chicken aspergillosis; and also the additive is abundant in vitamins and mineral matters, and is capable of effectively improving chicken immunity and disease-resistant capability and improving chicken survival rate, chicken does not contain harmful substance residues, and also the quality of chicken is improved.

Pharmaceutical compositions and methods for treating skin or mucus fungal infections of feet, nails, groin, hands, tongue and other locations are disclosed herein. The pharmaceutical composition disclosed herein comprises at least one triazole agent. Methods of treating tinea pedis, tinea manuum, tinea unguium, tinea cruris, tinea corpora, tinea versicolor, candidiasis, aspergillosis, onychomycosis, coccidiodomycosis, cryptococcal meningitis, histoplasmosis, hoof thrush, hoof rot, tongue and mouth lesions, seborrheic dermatitis, and combinations thereof, are also presently disclosed.

The invention discloses a traditional Chinese medicine preparation for preventing and treating avian aspergillosis. The traditional Chinese medicine preparation comprises the following components in parts by weight: 4-6 parts of herba houttuyniae, 2-4 parts of dandelions, 1-3 parts of decumbent bugle herbs, 1-3 parts of platycodon grandiflorum, 1-2 parts of lance asiabell roots, 1-3 parts of common andrographis herbs, 2-4 parts of honeysuckle, 1-3 parts of felwort, 1-3 parts of rhubarb, 1-3 parts of golden cypress and 1-3 parts of liquorice. After being crushed and sieved, the components are added with water, decocted, filtered and concentrated, encapsulated and sterilized to obtain a finished product. The traditional Chinese medicine preparation disclosed by the invention can effectively prevent and treat the avian aspergillosis, enhance poultry resistance, clear away heat and detoxify, and eliminate poultry body endotoxin. The traditional Chinese medicine preparation disclosed by the invention has the advantages of being simple in compatibility, convenient to use, low in cost, good in effects on preventing and treating the avian aspergillosis, reasonable and feasible.

The invention provides a saccharifying enzyme produced by fermenting draff mixed fluid and a method for producing alcohol by using the saccharifying enzyme to ferment kitchen and food rubbish, which relates to a method for producing saccharifying enzyme and a way for producing alcohol by using the saccharifying enzyme to ferment kitchen and food rubbish. The method for producing the saccharifying enzyme comprises the following procedures: I. mixing draff fluid with water, adding solid draff; II. disinfecting, adding aspergillosis fluid to ferment. The method for producing alcohol: a. mixing kitchen and food rubbish with waste water from a dining hall or tap water; b. adding saccharifying enzyme produced by above method, inoculating saccharomycetes, then fermenting under an anaerobic atmosphere; c. distilling the alcohol. The invention provides nitrous substances necessary for microbes to grow by using draff fluid and solid draff, which can act as traditional enzyme-producing culture medium. The invention can improve the activity of the saccharifying enzyme, and the produced saccharifying enzyme can be directly used for alcohol fermenting kitchen and food rubbish without purifying and separating, this can not only reduce the production cost for alcohol, but also change waste into treasure, improve the added value of kitchen and food rubbish, and avoid pollution from draff and draff fluid on environment.

The present invention provides monoclonal antibodies specific for members of the Aspergillus genus and uses thereof for increasing the survival rate of a subject infected with an Aspergillus species. The present invention also provides conjugates of alliinase with monoclonal antibodies specific for members of the Aspergillus genus that preserve the ability of the antibody to recognize the fungus as well as the activity of the enzyme to produce cytotoxic molecules of allicin from the substrate alliin. The invention further provides pharmaceutical compositions comprising the conjugates of the mAbs of the invention and alliinase and uses thereof in combination with the substrate alliin in the treatment of diseases cause by Aspergillus species, in particular invasive aspergillosis.

The invention discloses a strain BY25 of Pichia deserticola and a use method and applications thereof. The strain has a wide antibacterial spectrum, is used to control diseases of picked vegetables and fruits, and has a stable effect. The strain is preserved in China General Microbiological Culture Collection Center, CGMCC, and the preservation number is CGMCC No. 14906. The use method of Pichia deserticola comprises following steps: activating strains, using YPD to carry out fermentation culture, carrying out centrifugation, preparing bacterial suspension (1*10<8> cells/mL) from bacteria andsterile water; soaking fruits and vegetables (the apple, pear, grape, strawberry, orange, cherry tomato, etc.) in the bacterial suspension for 30 seconds, drying fruits and vegetables in the air, placing the fruits and vegetables in a fresh-keeping box, and storing the fruits and vegetables at a room temperature. The Pichia deserticola strain can control blue mold, gray mold, and black spot of theapple, blue mode and gray mold of the pear fruit, gray mold, aspergillosis, black spot, pink rot, and phoma disease of the grape, grey mold and aspergillosis of the straw berry, grey mold of orange,and grey mold and aspergillosis of the cherry tomato, reduces the loss caused by diseases after picking, and has a good application prospect.

The invention discloses a Pichia galeiformis bacterial strain BY27 used for control of postharvest diseases of fruits and vegetables and with broad antibacterial spectrum and stable effect and an application method thereof. The bacterial strain is preserved in CGMCC with the preservation number of CGMCC No.14907. The application method of the pichia pastoris comprises the following steps: activating the bacterial strain, carrying out fermental cultivation with YPD, centrifuging, and preparing bacterium suspension with the concentration of 1*10<8> cells/mL from thalli with sterile water; putting fruits and vegetables such as apples, pears, grapes, strawberries, citrus and cherry tomatoes into the bacterium suspension, soaking for 30 seconds, then taking out, and carrying out air drying; andputting into a preservation box, and storing at room temperature. The Pichia galeiformis bacterial strain disclosed by the invention can control penicilliosis, botrytis and black spot of the apples,penicilliosis and botrytis of pear fruits, botrytis, aspergillosis, black spot, fusariosis and cephalothecium roseum of the grapes, botrytis and aspergillosis of the strawberries, penicilliosis of thecitrus as well as botrytis and aspergillosis of the cherry tomatoes at the same time, and loss caused by the postharvest diseases is reduced, so that the Pichia galeiformis bacterial strain disclosedby the invention has a good application prospect.

As a result of the analysis by an SST-REX method so as to identify a target molecule for treating and testing an Aspergillus fumigatus infection, a YMAF1 protein has been found out, which is mainly localized in the cell wall of Aspergillus fumigatus. Moreover, it has been found out that YMAF1 protein-deficient Aspergillus fumigatus has reduced spore-forming ability and pathogenicity. Further, it has been found out that the survival rate of experimental mice having aspergillosis (invasive Aspergillus model mice) is improved by preparing and administering an antibody against the YMAF1 protein. Furthermore, it has been found out that Aspergillus fumigatus can be detected with a favorable sensitivity by an ELISA system using the antibody.

Allele specific primers and probes suitable for detecting allelic variants of human SP-A2 gene for applications such as molecular diagnosis, prediction of an individual's susceptibility, and/or the genetic analysis of SP-A2 gene in a population.

The invention discloses a feed and a traditional Chinese medicine composition for preventing and treating duck aspergillosis and a preparation method of the feed. The feed comprises a basic feed, microelement additives, vitamin additives and traditional Chinese medicine additives, wherein the traditional Chinese medicine additives comprise fritillaria cirrhosa, bamboo juice, tabasheer, platycodon grandiflorum, fructus perillae, aristolochia debilis sieb, lepidium apetalum willd, herba ardisiae japonicae, flos daturae, scutellaria baicalensis, coptis chinensis, radix sophorae flavescentis, barberry roots, horsetail, white mulberry root-barks, indigo naturalis, large-leaved gentian, dried orange peels, fructus citri, folium rhododendri daurici, thorny elaeagnus leaves, codonopsis pilosula, yams, fructus psoraleae, white perilla leaves and artemisia capillaries. The feed contains the traditional Chinese medicine additives and has the beneficial effects that the course of treatment of the duck aspergillosis is short, the drug residue content is low, the toxicity is reduced, the healing rate is high, the pollution is avoided, the effects of removing dampness to resolve sputum, relieving asthma, regulating vital energy and improving immunity are achieved, the duck aspergillosis does not easily relapse after being healed, and the cost and the price are low.

The invention belongs to the field of traditional Chinese medicine application, and especially discloses a traditional Chinese medicine used for treating respiratory medicine department bronchu spulmonary aspergillosis. According to a preparation method, radix scutellariae, perilla leaf, hibiscus bark, phellodendron, garlic, spreading hedyotis herb, laminaria japonica, radix stemonae, herba chelidonii, semen brassicae campestris, fructus akebiae, coptis chinensis, radix sophorae flavescentis, cortex cinnamomi, fennel, polygala fallax hemsl, and crocus sativus are taken as raw materials, and the traditional Chinese medicine application is prepared via mixing of the above raw materials at a certain weight ratio. The preparation method is simple; adsorption effect is excellent; drug effect lasting for a long term; curative effect is obvious; no side effect is caused; safety is high; production cost is low; and the traditional Chinese medicine is capable of alleviating symptoms of patients.

The invention discloses a pheasant aspergillosis preventing and control agent, and relates to the technical field of pheasant breeding. The pheasant aspergillosis preventing and control agent is prepared from, by weight, 15-20 parts of purslane herb, 10-15 parts of ginkgo leaves, 8-12 parts of Chinese honeylocust leaves, 6-10 parts of perilla leaves, 5-8 parts of chinaberry bark, 5-8 parts of honey-fried licorice roots, 4-6 parts of pepper leaves, 4-6 parts of a nutrition additive, 3-5 parts of broccoli, 3-5 parts of kelp, 3-5 parts of fresh ginger, 2-3 parts of radix scutellariae, 2-3 parts of cortex albiziae, 1-2 parts of tea seed cake powder, 1-2 parts of castor beans, 0.5-1 part of realgar and 100-150 parts of water. The preventing and control agent has a faint scent, can be directly dissolved into water or mixed into feed to feed sick chicken, takes effect fast, is good in treatment effect and free of side effects, can obviously relieve symptoms after being used three times, cures aspergillosis within seven days, and does not affect pheasant meat, nutrient value of eggs and food safety.