9-anthrone lactone compounds, preparing method and use thereof

A technology of compounds and uses, applied in the field of preparation of cell proliferation inhibitors, capable of solving problems such as no drugs

Inactive Publication Date: 2008-07-16
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research found that the 9-anthrone lactone compound has the activity of inhibiting cell proliferation and the activity of inhibiting the proliferation and migration of endothelial cells. At present, there are no reports on the chemical structure of the compound, the inhibition of cell proliferation, and the activity of inhibiting the proliferation and migration of endothelial cells. Therefore, there is no drug related to this on the market

Method used

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  • 9-anthrone lactone compounds, preparing method and use thereof
  • 9-anthrone lactone compounds, preparing method and use thereof
  • 9-anthrone lactone compounds, preparing method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Fermentative production and separation and purification of embodiment 1 compound I

[0013] 1 Fermentation production

[0014] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take an appropriate amount of Aspergillus glaucus HB1-19 (Aspergillus glaucus HB1-19) (preservation number is: CCTCC M 206022), inoculate it on Chase's solid slant medium, and inoculate it at 28 degrees Celsius. Cultured in the incubator for 4 days.

[0015] Take an appropriate amount of Aspergillus glaucus HB1-19 (Aspergillus glaucus HB1-19) that has been cultured on a slant for 4 days, and inoculate it into 100 mL of culture fluid [medium composition (g / L): maltose 20.0, mannitol 20.0, monosodium glutamate 10.0, KH 2 PO 4 0.5, MgSO 4 0.3, yeast extract 3.0, pH natural] in a 500mL Erlenmeyer flask, cultured on a shaking table at 28°C and 160 rpm for 7 days to obtain mycelium and fermentation broth.

[0016] 2 Obtaining the extra...

Embodiment 2

[0023] The test of embodiment 2 in vitro antitumor activity

[0024] Cell Proliferation Inhibitory Activity Test

[0025] 1 Experimental samples and experimental methods

[0026] Preparation of the tested sample solution The test sample is the pure compound I isolated and refined in Example 1 above. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0027] Cell lines and subculture of cells Use cancer cell lines such as human lung cancer A549 cells, human liver cancer BEL-7402 cells, human leukemia HL60 cells and mouse leukemia P388 cells. All kinds of cells were subcultured in RPMI-1640 medium containing 10% FBS at 32°C (P388 cells) or at 37°C (A549, HL60 and BEL-7402 cells) in an incubator filled with 5% carbon dioxide .

[0028] Cell Proliferation Inhibitory Activity Test Method

[0029] Lissamine rhodamine B (SRB) method Take human lung cancer A549 cells and human liver can...

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Abstract

A 9-anthrone lactone compound is prepared from Aspergillosis glaucus HB1-19 extracted from marine specimen. It can be used as the inhibitors of cell reproduction, angiogenesis and tumor.

Description

Technical field: [0001] The present invention relates to a method for producing 9-anthrone lactone compounds with Aspergillus glaucus HB1-19 (Aspergillus glaucus HB1-19) (preservation number: CCTCC M206022); the present invention also relates to the use of such compounds in the preparation of cell proliferation inhibitors . Use in an agent for inhibiting endothelial cell proliferation or an antitumor agent. Background technique: [0002] There are many literature reports on 9-anthrone compounds, but there are no reports on the derivatives of this 9-anthrone lactone. The inventors found that the crude extract of the liquid fermentation product of Aspergillus glaucus HB1-19 (Aspergillus glaucus HB1-19) (preservation number: CCTCC M206022) was ultrasonically crushed and detected by chemical TLC and HPLC fingerprints, and it was found that quinone The content of ketones and ketones is abundant, so their chemical constituents were studied. The research found that the 9-anthrone...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/08A61K31/352A61P35/00A61P9/14A61P43/00
Inventor 刘红兵杜林朱伟明顾谦群朱天骄方玉春刘培培
Owner OCEAN UNIV OF CHINA
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