Glucoamylase produced by fermentation of wine lees miscible liquid and method for producing alcohol by fermenting restaurant garbage with this glucoamylase

A technology of kitchen waste and mixed liquor, applied in fermentation, hydrolase, biofuel and other directions, can solve problems such as high cost and environmental pollution

Inactive Publication Date: 2008-03-12
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of the high cost of the existing alcohol preparation method and the environmental pollution caused by distiller's grains and lees, the present inven

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0008] Specific Embodiment 1: The method for fermenting distiller's grains to produce glucoamylase in this embodiment is realized through the following steps: 1. Mix distiller's grains with water at a mass ratio of 1 to 2:1, and add 0.5 to 1.5 % (w / w), solid distiller's grains with a water content of 55-70%, to obtain a distiller's grains mixture; 2. Sterilize the distiller's grains mixture at 115-121°C for 15-20 minutes, and then add 6 ~10ml Aspergillus liquid, the proportion of spores inserted into the concentration of 1 × 10 7 ~3×10 7 Aspergillus per ml, shake the flask for 84-96 hours under the conditions of 30-35°C and 100-200r / min to ferment for 84-96 hours to obtain the saccharification enzyme liquid.

[0009] Comparative Experiment:

[0010] Mix distiller's grain waste liquid with water at a mass ratio of 1 to 2:1, add bran, distiller's grains (water content 70%) and dried distiller's grains of 0.8% of the weight of the mixed solution respectively, wherein the bran i...

specific Embodiment approach 2

[0015] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in Step 1, 0.8-1.2% (w / w) solid distiller's grains with a water content of 58-67% are added to the mixed liquor. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0016] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step 1, 1% (w / w) solid distiller's grains with a water content of 60% are added to the mixed liquor. Other steps are the same as in the first embodiment.

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PUM

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Abstract

The invention provides a saccharifying enzyme produced by fermenting draff mixed fluid and a method for producing alcohol by using the saccharifying enzyme to ferment kitchen and food rubbish, which relates to a method for producing saccharifying enzyme and a way for producing alcohol by using the saccharifying enzyme to ferment kitchen and food rubbish. The method for producing the saccharifying enzyme comprises the following procedures: I. mixing draff fluid with water, adding solid draff; II. disinfecting, adding aspergillosis fluid to ferment. The method for producing alcohol: a. mixing kitchen and food rubbish with waste water from a dining hall or tap water; b. adding saccharifying enzyme produced by above method, inoculating saccharomycetes, then fermenting under an anaerobic atmosphere; c. distilling the alcohol. The invention provides nitrous substances necessary for microbes to grow by using draff fluid and solid draff, which can act as traditional enzyme-producing culture medium. The invention can improve the activity of the saccharifying enzyme, and the produced saccharifying enzyme can be directly used for alcohol fermenting kitchen and food rubbish without purifying and separating, this can not only reduce the production cost for alcohol, but also change waste into treasure, improve the added value of kitchen and food rubbish, and avoid pollution from draff and draff fluid on environment.

Description

technical field [0001] The invention relates to a method for producing glucoamylase and a method for producing alcohol by using the glucoamylase to ferment kitchen garbage. Background technique [0002] As the energy problem becomes more and more prominent, the research on alternative energy sources has been paid more and more attention, among which fuel alcohol has been developed rapidly. People have carried out research on the production of fuel alcohol from various raw materials, such as cellulose raw materials, aged grains, molasses, etc. The patent with the publication number CN1948498 discloses a method for producing fuel ethanol from food waste. Although the raw material has a price advantage, industrial enzymes are required in the production process, resulting in high production costs. [0003] The development of biomass energy such as fuel alcohol has also brought problems and troubles to the society, especially the remaining solid distiller's grains and liquid dist...

Claims

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Application Information

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IPC IPC(8): C12N9/24C12P7/06
CPCY02E50/17Y02E50/10
Inventor 汪群慧马鸿志尹玮宫丽娟
Owner HARBIN INST OF TECH
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