Saccharomyces ellipsoideus for preventing and treating postharvest diseases of fruits and vegetables and preparation and use methods thereof

A technology for Saccharomyces cerevisiae and post-harvest diseases, applied in the field of Saccharomyces cerevisiae, can solve the problems that the biocontrol effect is only verified on a small number of fruits, and the lack of antibacterial spectrum strains, etc., achieves significant social and ecological benefits, stable properties, and safety sex high effect

Active Publication Date: 2018-04-06
山东凯普菲特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although there are nearly a hundred species of antagonistic yeasts reported at home and abroad, the biocontrol effects of most antagonistic yeasts have only been verified on a few fruits.
However

Method used

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  • Saccharomyces ellipsoideus for preventing and treating postharvest diseases of fruits and vegetables and preparation and use methods thereof
  • Saccharomyces ellipsoideus for preventing and treating postharvest diseases of fruits and vegetables and preparation and use methods thereof
  • Saccharomyces ellipsoideus for preventing and treating postharvest diseases of fruits and vegetables and preparation and use methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Biological properties of the wine Saccharomyces ellipsoideus strain BY36

[0020] 1. Morphological features

[0021] (1) YPDA medium (1% yeast extract powder, 2% peptone, 2% glucose, 1.8% agar, sterilized at 121°C for 20 minutes) was cultured at 26°C for 48h, and the colonies were round and white with smooth and round edges. The cell shape is ellipsoidal.

[0022] (2) After culturing in YPDA liquid medium for 24 hours, no mold was formed, the bacterial solution was turbid, and there was precipitation. Microscopically, the yeast cells were oval and budded.

[0023] 2. Molecular biological identification

[0024] Use the universal forward primer NL-1 (5'-GCATATCAATAAGCGGAGGAAAAG-3') and reverse primer NL-4 (5'-GGTCCGTGTTTCAAGACGG-3') to perform PCR amplification of yeast 26S rDNA D1 / D2 region nucleic acid sequence, and the PCR product The sequencing results were entered into the website www.NCBI.nlm.nih.gov for BLAST, the homologous sequences were downloaded...

Embodiment 2

[0026] The inhibitory effect of implementation example 2 wine Saccharomyces cerevisiae BY36 to apple blue mold and gray mold

[0027] 1. Experimental protocol

[0028] Take out Saccharomyces cerevisiae BY36 from the -80°C refrigerator, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and pick a single Colony into YPD liquid medium, culture at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on a hemocytometer to prepare the concentration 1×10 8 cells / mL of Saccharomyces cerevisiae BY36 suspension.

[0029]Activate Penicillium expansum or Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water. 4 cells / mL of Penicillium or Botrytis cine...

Embodiment 3

[0036] Implementation Example 3 The inhibitory effect of wine Saccharomyces cerevisiae BY36 on pear fruit botrytis

[0037] 1. Experimental protocol

[0038] Take out Saccharomyces cerevisiae BY36 from the -80°C refrigerator, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and pick a single Colony into YPD liquid medium, culture at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on a hemocytometer to prepare the concentration 1×10 8 cells / mL of Saccharomyces cerevisiae BY36 suspension.

[0039] Activate Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water. 4 cells / mL of Botrytis cinerea spore suspension.

[0040] Healthy and unda...

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Abstract

The invention discloses Saccharomyces ellipsoideus BY36, with broad bacteriostasis spectrum and stable effect, for preventing and treating postharvest diseases of fruits and vegetables and a use method and use thereof. The strain has a collection strain serial number of CGMCC No. 14910 in General Microorganisms Center of China Committee for Culture Collection of Microorganisms. The use method of the Saccharomyces ellipsoideus comprises the steps: activating the strain, carrying out fermentation culture with YPD, carrying out centrifugation, and preparing a 1*10<8>cell/mL bacterial suspension from thalli and sterile water; placing the fruits and vegetables such as apples, pears, grapes, strawberries, oranges or cherry tomatoes into the bacterial suspension, carrying out soaking for 30 seconds, then, taking out the fruits and vegetables, and drying the fruits and vegetables in the air; and placing the fruits and vegetables into a fresh-keeping box, and carrying out storage at normal temperature. The Saccharomyces ellipsoideus strain can be used for simultaneously controlling blue molds and gray molds of apples, gray molds of pears, gray molds, aspergillosis, black spots, anthrax andpink rot of grapes, gray molds of strawberries, blue molds of oranges and gray molds and aspergillosis of cherry tomatoes, and loss caused by the postharvest diseases is reduced, so that the Saccharomyces ellipsoideus strain has a very good application prospect.

Description

technical field [0001] The invention relates to the field of biological control of post-harvest diseases of fruits and vegetables, in particular to a wine yeast (Saccharomyces ellipsoideus) used for the biological control of post-harvest diseases of fruits and vegetables. The main postharvest diseases have significant control effects. Background technique [0002] Although the quality deterioration of fresh fruits and vegetables is affected by many factors, disease is the main reason. Among them, rot and deterioration caused by fungal diseases is the most serious factor in postharvest fruit loss. Although postharvest diseases of fruits and vegetables can be prevented and controlled in many ways such as agricultural control, physical control, chemical control and biological control, the main measure at present is chemical control (Eckert & Ogawa, 1985, 1988). However, long-term use of chemical pesticides not only leads to the development of drug resistance of pathogenic bac...

Claims

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Application Information

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IPC IPC(8): C12N1/16A01N63/04A01P3/00A23B7/155C12R1/85
CPCA23B7/155A01N63/30C12N1/16C12N1/185C12R2001/85
Inventor 王友升任向峰马国为黄津津李丽萍姚婷
Owner 山东凯普菲特生物科技有限公司
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