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Debaryomyces hansenii strain and applications thereof

A technology for yeast and strains, used in applications, fungicides, fungi, etc.

Inactive Publication Date: 2015-10-21
XINJIANG XIANBAOLAI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Debaryomyces hansenii (Debaryomyces hansenii) is a non-pathogenic yeast with salt tolerance, drought tolerance and osmosis tolerance. It is widely distributed in nature, but there is no report on its application in the control of Botrytis cinerea in China

Method used

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  • Debaryomyces hansenii strain and applications thereof
  • Debaryomyces hansenii strain and applications thereof
  • Debaryomyces hansenii strain and applications thereof

Examples

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Effect test

Embodiment 1

[0031] Embodiment 1 Debaryomyces hansenii of the present invention ( Debaryomyces hansenii ) Isolation and strain identification of XBL01

[0032] 1. Sample collection

[0033] Grapes are collected from organic orchards in northern China.

[0034] 2. Isolation and screening of strains and antagonistic screening

[0035] Isolate strains from organic grapes, use conventional gradient dilution coating to isolate, use PDA (containing streptomycin sulfate 25mg / L) and YPD agar (containing streptomycin sulfate 25mg / L) a total of two culture media at 25 ℃ respectively cultured under the same conditions, pick the colonies with large differences in colony morphology and can be purely cultivated, and purify and preserve them on YPD agar medium (containing streptomycin sulfate 25mg / L), and use postharvest Botrytis cinerea as the target pathogen to carry out antagonistic antibacterial treatment. hansenii strain with strong antibacterial activity, named it XBL01, and preserved it with ...

Embodiment 2

[0042] Example 2 Low temperature tolerance test of Debaria hansenii strain XBL01 CGMCC No.10464 of the present invention

[0043] At present, Debaryomyces hansenii ( Debaryomyces hansenii ) The suitable growth temperature range is generally 20-30°C, and it can grow at 37°C. But the Debaryomyces hansenii described in this application ( Debaryomyces hansenii ) The suitable growth temperature of XBL01 CGMCC No.10464 strain is 15-28°C, but it does not grow at 37°C. Its suitable growth temperature determination experimental method is as follows:

[0044] Add an equal amount of Debaria hansenii ( Debaryomyces hansenii ) XBL01 CGMCC No.10464 strain (10 6 cells) bacteria were inoculated into conical flasks containing 1 liter of YPD liquid medium (containing streptomycin sulfate 25mg / L), and then incubated at 4°C, 10°C, 15°C, 20°C, 25°C, 28°C, and 30°C respectively. Shake flask culture (180-200rmp) at ℃ for 96h. Among them, each treatment was sampled once every 12 hours, and t...

Embodiment 3

[0045] Example 3 Debariae hansenii XBL01 CGMCC No.10464 strain antibacterial effect test of the present invention

[0046] Pathogens tested:

[0047] Botrytis cinerea (Botrytis cinerea) Botrytis cinerea Per. ex Fr).

[0048] Postharvest Botrytis cinerea was selected as the indicator fungus, and the plate confrontation culture method was adopted.

[0049] The specific operation is as follows: Debaria hansenii ( Debaryomyces hansenii ) XBL01 CGMCC No.10464 strain was cultured on PDA medium at 25°C for 48 hours. Cultivate the indicator fungus on a PDA plate at a constant temperature of 25°C for 3-4 days, and use a sterile stainless steel puncher with a diameter of 0.7cm from the edge of the colony to make agar sheets with bacteria; Inoculate it at the center of the PDA plate, and at the same time, use a sterile inoculation loop to streak Debaria hansenii ( Debaryomyces hansenii ) XBL01 CGMCC No.10464 strain, with the plate only inoculated with pathogenic bacteria as a con...

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Abstract

The present invention provides a strain of Debaryomyces hansenii, wherein the strain is Debaryomyces hansenii XBL01 and has the preservation number of CGMCC No.10464. The present invention further provides applications of the strain and corresponding bacterial agents in preparation of pathogenic bacteria inhibitors, disease inhibitors, bio-pesticides and fruit fresh-keeping agents. According to the present invention, the Debaryomyces hansenii XBL01 CGMCC No.10464 strain can be well used for prevention and control of pathogenic bacteria, especially for prevention and control of Botrytis cinerea, can be used for preparation of fruit fungicides or fresh-keeping agents, does not have side effects, and can not cause great pressure on the environment and the ecology.

Description

technical field [0001] The invention belongs to the technical field of microbial agents, and in particular relates to a Debaryomyces hansenii strain and its application. Background technique [0002] Post-harvest fruit diseases including fungal and bacterial diseases are an important class of diseases, which cause a large reduction in fruit yield, seriously affect the edible value and market value of fruits, and lead to a decrease in the economic income of fruit farmers. In developed countries, the losses caused by post-harvest diseases account for more than 15% of the total amount of fruits. In developing countries, due to the lack of necessary prevention and control technology and equipment, the losses caused by post-harvest diseases account for more than 30% of the total. . The occurrence of post-harvest fruit diseases is the main cause of large-scale fruit rot in circulation and storage. The occurrence of postharvest fruit diseases has seriously affected modern agricul...

Claims

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Application Information

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IPC IPC(8): C12N1/16A01N63/04A01P3/00A23B7/155C12R1/645
CPCA23B7/155A01N63/30C12N1/16C12N1/145C12R2001/645
Inventor 张殿朋施文杰杨建伟
Owner XINJIANG XIANBAOLAI BIOTECH CO LTD
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