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Irpex lacteus LL210, application thereof and biocontrol microbial agent

A technology of Raketodon albicans, LL210, applied in the field of microorganisms

Active Publication Date: 2021-06-04
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the effect of R. albicans on phytopathogenic fungi

Method used

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  • Irpex lacteus LL210, application thereof and biocontrol microbial agent
  • Irpex lacteus LL210, application thereof and biocontrol microbial agent
  • Irpex lacteus LL210, application thereof and biocontrol microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Isolation and identification of antagonistic bacteria

[0024] In the present invention, a strain of Raketodon albus LL210, which antagonizes tomato pathogenic fungi, is isolated from tomato leaves.

[0025] Select healthy leaves from diseased tomato plants for sampling, put them in sterilized ziplock bags prepared in advance, and take them back to the laboratory for separation. In the ultra-clean workbench, first wash the material in sterile water, then disinfect with 75% alcohol for 10-30s, rinse with sterile water for 3 times, then disinfect with mercuric chloride for 15s, rinse with sterile water for 3 times, and in sterile Drain excess moisture from the paper. Cut the material into square pieces of about 1 cm with a scalpel, insert the wound into the PDA medium with the wound facing down, and cultivate in a constant temperature incubator at 28°C for several days. in a 28°C constant temperature incubator for pure culture.

[0026] The morphological feat...

Embodiment 2

[0032] Example 2 Inhibitory effect of R. albicans LL210 on mycelial growth of tomato gray mold pathogen

[0033] This example verifies the antagonistic effect of Irpex lacteus LL210 on a phytopathogenic fungus, which is Botrytis cinerea Pers.

[0034] On a PDA medium plate with a diameter of 9cm, place a 0.8cm-diameter fungus cake on one side of the plate, and on the other side place a tomato gray mold pathogen cake of the same size, with an interval of 4.5cm between the two bacteria cakes , placed in a 28 ℃ constant temperature incubator, and the antibacterial rate was counted on the 7th to 14th day respectively.

[0035] The results are shown in the following table, R. albicans has obvious inhibitory effect on the growth of tomato gray mold mycelium ( figure 2 ), the antibacterial rates during 7-14 days of culture are:

[0036] time 7d 8d 9d 10d 11d 12d 13d 14d Bacteriostatic rate 64.4％ 66.7％ 73.3％ 73.3％ 81.1％ 86.7％ 88.9％ 100％

Embodiment 3

[0037] Example 3 Inhibitory effect of H. albicans LL210 on mycelial growth of tomato late blight pathogen

[0038]This example demonstrates the antagonism of Irpex lacteus LL210 against a phytopathogenic fungus, P. infestans (Mont.) De Bary.

[0039] On a PDA medium plate with a diameter of 9cm, place a 0.8cm-diameter fungus cake on one side of the plate, and on the other side place a tomato late blight pathogenic bacteria cake of the same size, with an interval of 4.5cm between the two bacteria cakes. Placed in a 28 ℃ constant temperature incubator for cultivation, and the antibacterial rate was counted on the 7th to 14th day respectively. The results are shown in the following table, R. albicans LL210 has obvious inhibitory effect on the growth of Phytophthora tomato mycelium ( image 3 ), and the antibacterial rates during 7-14 days of culture are:

[0040] time 7d 8d 9d 10d 11d 12d 13d 14d Bacteriostatic rate 65.6％ 68.9％ 77.8％ 82.2％ 85.6％ 91....

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Abstract

The invention relates to irpex lacteus LL210, application thereof and a biocontrol microbial agent. The irpex lacteus provided by the invention is classified and named as Irpex lacteus, and the preservation number of the Irpex lacteus is CGMCC No.21057. A strain is separated from tomato leaves, and is non-toxic and free of pathogenicity. A plate confrontation test shows that the strain can effectively inhibit mycelial growth and spore germination of tomato botrytis cinerea and tomato late blight, and can be used for antagonizing plant pathogenic fungi tomato botrytis cinerea and / or phytophthora. The biocontrol microbial agent prepared from the irpex lacteus LL210 is used for preventing and treating tomato diseases, the prevention and treatment effects on tomato botrytis cinerea and late blight reach 81.35% and 87.68%, the biocontrol microbial agent has a good disease prevention effect and good compatibility with the environment, and a new microbial resource is provided for biological prevention and treatment of diseases of plants such as tomatoes.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to Raketodon albicans LL210 and its application, and a biocontrol agent. Background technique [0002] The production mode of facility vegetables in my country has the characteristics of low input, low energy consumption, intensification, and year-round cultivation, resulting in serious obstacles to continuous cropping and high incidence of diseases, which has become a bottleneck restricting the sustainable utilization of facility vegetables in my country. For example, tomato gray mold caused by tomato botrytis cinerea infection caused by Fusarium oxysporum infection, tomato late blight caused by Phytophthora. At present, the control of pathogenic microorganisms mainly includes physical control, chemical control and biological control. Although these chemicals have quick effects, long-term use will lead to increased resistance of these pathogens to the drugs, and ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P3/00A01G22/05C12R1/645
CPCC12N1/14A01N63/30A01G22/05
Inventor 陈双臣刘爱荣毋美娟付亚伦张少杰彭晓华杨政坤耿李佳宏宋智煜赵同淑黄茜茜
Owner HENAN UNIV OF SCI & TECH
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