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Bacillus subtilis J-5 as well as biological agent and application thereof

A technology of Bacillus subtilis and biological agents, applied in the direction of biocides, biochemical equipment and methods, applications, etc., can solve the problems of unsatisfactory antibacterial effect, short duration of action, narrow antibacterial spectrum, etc., to avoid Residue and contamination, long duration of action, fast-acting effect

Active Publication Date: 2016-08-31
秦皇岛禾苗生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are some biocontrol agents used for the biological control of Botrytis cinerea, but most of them have unsatisfactory antibacterial effects, narrow antibacterial spectrum, and short duration of action

Method used

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  • Bacillus subtilis J-5 as well as biological agent and application thereof
  • Bacillus subtilis J-5 as well as biological agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Isolation and Screening of Bacteria with Antagonism to Cucumber, Tomato, Eggplant, Sweet Pepper, Strawberry Botrytis

[0020] The rhizosphere soil of healthy plants in cucumbers, tomatoes, eggplants, sweet peppers, and strawberry plots with severe gray mold disease was collected as test materials. Bacteria were isolated from the samples in the laboratory, and multiple isolations were performed on LB medium Purify and obtain the pure cultured colonies, use Botrytis cinerea as the indicator strain, and use the confrontation test method to screen the strains with high antagonistic performance.

[0021] Specific steps: the above soil samples come from the cultivation fields of cucumbers, tomatoes, eggplants, sweet peppers, and strawberries infected with gray mold in Hebei Province, Ningxia, Shandong, Shanxi and other places. cultured in a constant temperature incubator at 28°C. The above-mentioned medium was prepared according to the records in the "Microbial Cul...

Embodiment 2

[0024] Example 2 Determination of the genetic stability of antagonistic strains

[0025] Strain J-5 was subcultured on PB medium by streaking method. After 20 generations of culture, the antagonistic effect of subcultured J-5 strain on Botrytis cinerea was measured by plate confrontation method. The original J-5 strain was used as In contrast, the antagonistic strain has the same antagonistic effect as the original strain after 20 generations of culture, indicating that the antagonistic strain has good genetic stability, and the antagonistic strain with stable antibacterial effect is cultured in PB medium, and the bacterial liquid is drawn for - Store in glycerol at 80°C.

Embodiment 3

[0026] Example 3 Identification of antagonistic strains

[0027] According to the experimental content and experimental methods recorded in the "Common Bacteria System Identification Manual", the screened strain J-5 with the preservation number CGMCC No.11750 was identified, and the colony morphology observation was carried out on PB medium. After streak inoculation, culture at 28°C for 24 hours to observe the colony morphology. The colonies of the strain with the preservation number CGMCC No.11750 are nearly round, with gnawing edges, umbilicus-like protrusions, wrinkled surfaces, milky white color, dull and opaque, and are not tightly combined with the medium. Pick and culture the bacterial lawn for 24 hours and observe it under the 100 times oil lens of an optical microscope after Gram staining. It can be seen under a microscope that the strain with the preservation number CGMCC No.11750 is a bacillus, a Gram-positive bacterium, with mesozoic or near-mesophytic spores, enl...

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Abstract

The invention provides bacillus subtilis having a preservation number of CGMCC No.11750, a microbial agent containing the bacillus subtilis and application of the bacillus subtilis or microbial agent in prevention and control of grey mold of tomatoes, cucumbers, strawberries and other fruits and vegetables. The bacillus subtilis having the preservation number of CGMCC No.11750 and the microbial agent containing the bacillus subtilis have a strong antagonizing effect on botrytis cinerea, have quick response and have long prevention lasting period. A corresponding microbial preparation can be prepared by utilizing the screened bacillus subtilis strains, and occurrence of grey mold on tomatoes, cucumbers, strawberries and other vegetables can be prevented and controlled by the bacillus subtilis after the prepared microbial agent is applied to the field, so that chemical pesticide application in the fruit and vegetable production process can be reduced.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a bacillus subtilis J-5 and its biological agent and application. Background technique [0002] Botrytis cinerea, also known as Botrytis cinerea, is a worldwide distribution of fungi, it is a wide host, can cause more than 200 known plants (such as fruits, vegetables and flowers) Botrytis cinerea necrotrophic pathogenic fungi. Overwinter in the soil with sclerotia, conidia and mycelium along with the sick tissue, invade the host through wounds, natural orifices and young tissues, and produce a large number of conidia after the onset, spread by wind and rain for many times Re-infection can cause gray mold, which can affect flowers, fruits, leaves, and stems, causing wilting and death of crops, affecting yield and quality. It is one of the most difficult and important diseases to control in production. [0003] On cucumbers, tomatoes, eggplants, sweet peppers, str...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125
CPCA01N63/10C12N1/205C12R2001/125
Inventor 贾振华金伟伟黄亚丽宋水山马宏宋聪张立文
Owner 秦皇岛禾苗生物技术有限公司
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