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58results about How to "Drug resistant" patented technology

Production process of biological-organic-inorganic ternary compound fertilizer

The invention provides a production process of a biological-organic-inorganic ternary compound fertilizer which is prepared by the following steps: mixing crop stalks, such organic components as livestock manures and active biological agents, regulating the carbon nitrogen ratio from 26 to 35, PH from 5.5-8.2 and moisture from 40% to 65% according to the organic matter contents of the crop stalksand the livestock manures, fermenting the mixture at minus15-75 DEG C for 20-50 days and ensuring the living bacteria count to be 0.03-0.2 billion/g, carrying out turning and oxygenating, fermenting in deep tanks and grinding on the mixture to prepare the biological organic fertilizers, mixing the biological organic fertilizers with the inorganic fertilizers and trace elements and simultaneously adopting the low-temperature drying process after extruding granulation and the biological agent spraying and cooling technology. The method can greatly shorten the decay period, ensure little nutrientloss and little generating capacity of sour gases in the processes of fermentation and decay and complete decay and fermentation, can enhance the stress resistance of the crops, ensure obvious disease-resistant effects and high survival rates of specific functional bacteria and has wide application. Through field contrast tests, both the production and income are increased by 10-25% by applying the biological organic fertilizer.
Owner:辽宁元亨生物科技有限公司

High-throughput molecular detection of Fusarium graminearum on carbendazim medicament-resistant gene frequency

The invention belongs to high-throughput molecular detection of Fusarium graminearum on carbendazim medicament-resistant gene frequency, which can be used for monitoring the medicament resistance of the Fusarium graminearum and early warning medicament-resistant epiphytotic disease of the Fusarium graminearum. The invention provides medicament-resistant high-throughput detection technology constructed based on the fact that over 97 percent of Fusarium graminearum medicament-resistant genetype to carbendazim results from mutation of the 167th locus of beta2-microtubulin gene. The detection method mainly comprises the following three steps of: (1) respectively extracting known sensitive and medicament-resistant strains and genome DNA of samples to be detected; (2) performing specific real-time quantitative PCR reaction and establishing a standard curve; and (3) contrasting the standard curve to solve the medicament-resistant gene frequency in the detected samples. The method has the characteristics of high throughput, rapidness and accuracy. The sensitivity of detecting the medicament-resistant gene frequency is millionth to one hundred thousandth, and the accuracy rate is over 96 percent.
Owner:NANJING AGRICULTURAL UNIVERSITY

High throughput real-time detection technology for drug resistance gene frequency of Sclerotinia sclerotiorum population

The present invention belongs to a real-time detection method of the frequency of drug resistance genes in Sclerotinia sclerotiorum syngen, which can be used for drug resistance monitoring and popularity warning of carbendazim and other benzimidazole fungicide resistance in sclerotinia sclerotium disease of cole and the like crops. The detection method is divided into three major steps, the first step: respectively extracting the nuclear genome DNA of known drug-resistant strains, sensitive strains and sample to be measured; the second step: using the specific detection primers of drug-resistant strains and Sclerotinia sclerotiorum strains for real-time quantitative PCR reactions, respectively establishing the standard curves of the drug-resistant strains and Sclerotinia sclerotiorum strains detection; the third step: sample to be measured respectively using the two specific primers for real-time quantitative PCR reactions, contradistinguishing with the established standard curves, obtaining the corresponding numbers of the drug-resistant strains and sensitive strains and the proportion of the drug-resistant strains in the total number of the Sclerotinia sclerotiorum. The use of real-time quantitative PCR technology can perform high-throughput, rapid and accurate quantitative detection of the number of field drug-resistant Sclerotinia sclerotiorum and its proportion in the pathogen syngens, which has high-throughput characteristic. Compared with the conventional biometrics and general PCR detection of drug-resistant strains, the invention is time-saving, cost-saving and fast. The whole process from directly extracting the genome DNA from the sclerotia, disease spots and spores collected to real-time quantitative-PCR detection is only 6h needed, and the detection accuracy rate is more than 96%.
Owner:NANJING AGRICULTURAL UNIVERSITY

Composite material taekwondo pad with sandwich made from XPE

The invention discloses a composite material taekwondo pad with a sandwich made from XPE. The composite material taekwondo pad comprises a non-slip surface, an intermediate layer and a non-slip bottom surface, which are sequentially arranged from top to bottom, wherein the non-slip surface is made of a PVC non-slip composite layer with non-slip patterns; the non-slip bottom surface is made of a PVC non-slip composite layer with non-slip patterns or an embossing XPE layer with convex-concave patterns; the intermediate layer is formed by multiple XPE layers; and the density of the adjacent XPE layers is the same or different. According to the invention, the intermediate layer is prepared by compounding the multiple XPE layers with the same or different density through a heat seal process, so that the made pad has the advantages of high comfort level, moderate hardness and high impact resistance. According to the non-slip patterns on the non-slip surface and the non-slip bottom surface, the friction force of the whole pad is increased, and the comfort level of a user is guaranteed on the basis that the friction is increased. A taekwondo site is laid by the composite material taekwondo pads, the composite material taekwondo pad is high in overall flatness, high in safety, good in service effect and high in durability and is suitable for wide popularization and application, and the stability and safety of athletes in the sports process are effectively guaranteed.
Owner:SHANDONG TAISHAN SPORTS EQUIP
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