22 results about "Codling moth" patented technology

The application discloses a kind of cydia pomella larva's food lure composition and application thereof.It includes the following weight parts of component: isoquercitrin 0.02~0.07 parts, phlorizin 0.6~0.9 parts, cyanidin-3-O-galactoside 0.01~0.02 parts, delphinidin-3-O-galactose 0.05~0.12 parts.The food lure component provided in the application has low dosage and low price, can improve the behavior regulation effect of lure core on larvae, reduce the production cost, and is suitable for popularization and promotion.

The invention relates to the technical field of pest control, in particular to a method for evaluating habitat suitability and diffusion trend of codling moths and application. The method comprises the following steps: S1, collecting occurrence distribution data of codling moths; s2, acquiring a plurality of pieces of bioclimate variable data, human activity influence indexes and apple yield data of codling moth occurrence distribution, constructing a multi-dimensional environmental factor data set according to the plurality of pieces of bioclimate variable data, the human activity influence indexes and the apple yield data, and performing cross validation on the data set; and S3, based on a Biomod2 set model, establishing a plurality of single ecological models according to the occurrence distribution data and the multi-dimensional environmental factor data set, selecting high-accuracy models according to TSS and AUC values of the single models, integrating the models to generate a set model, and obtaining a habitat suitability result of codling moths and a contribution rate of main environmental factors. Scientific reference is provided for early warning, monitoring and prevention of codling moths.

The utility model discloses a trapping device for preventing insects from laying eggs, which comprises a wool felt (1) compressed into a corrugated shape, a group of bristles (2) sewed at two ends of the wool felt (1) respectively, a support rod (3) and a plastic film (4). The egg laying trapping device induces codling moths to lay eggs in the device, concentratedly destroys eggs and trapped adults, has the advantages of being simple in structure and capable of being recycled, can reduce use of chemical pesticides, guarantees food safety and quality, and reduces prevention and control cost while controlling the number of pests.

The invention relates to the technical field of agricultural biology, in particular to application of a neuropeptide gene CpILP6 in relieving diapause of codling moths. After siRNA is microinjected to silence CpILP6 in a targeted manner, the diapause state of CpILP6 is changed and tends to a normal development phenotype; the research result of the invention provides a theoretical basis for defining the regulation function of the neuropeptide gene CpILP6 in the diapause induction and maintenance process of codling moth.

The invention discloses a method for determining the resistance of different hosts to codling moth, which comprises the following steps: S1, making a codling moth egg-laying device, a codling moth egg-hatching device and a newly emerged codling moth breeding device; S2, collecting hosts of different varieties; S3, determining the egg-laying selection of codling moth on different hosts; S4, inoculating newly hatched larvae; S5, obtaining data; and S6, comprehensive analysis. The method for obtaining data has the following specific operations: S51, taking newly hatched larvae and inserting them into the host; S52, recording the time when the larvae fall off the fruit; S53, recording and calculating the emergence rate; S54, transferring a pair of newly emerged codling moths fed on the same variety of hosts to the newly emerged codling moth breeding device for pairing, and recording the egg-laying amount. The invention can simply and effectively determine the egg-laying selection of codling moth adults on different varieties of hosts, and various indicators in the growth, development and reproduction process of codling moth, so as to achieve the purpose of comprehensively judging and evaluating the resistance of different hosts to codling moth.

The invention belongs to the field of insect breeding, and specifically discloses the application of linalool in improving the mating competitiveness of sterile male codling moths, and specifically uses linalool to fumigate sterile male codling moths, wherein the concentration of linalool is 70-96 μL / m 3 Compared with the prior art, the present invention confirms that linalool fumigation treatment can improve the mating competitiveness of irradiated sterile male codling moth, and further improve the mating competitiveness and fitness of sterile male codling moth in the field.

The present invention belongs to the field of biotechnology and mainly relates to a LAMP detection primer and method for the mutation of the GABA receptor of the codling moth. The primers include a forward outer primer F3, a reverse outer primer B3, a forward inner primer FIP, a reverse inner primer BIP, a forward loop primer Loop-F, and a reverse loop primer Loop-B. Using the detection primers provided by the present invention, a rapid detection method for the A302S mutation of the GABA receptor of the codling moth can be established, and further determine whether the codling moth is resistant to abamectin. The LAMP detection primer set can be used for the detection of the mutation of the GABA receptor of the codling moth, with high sensitivity, a large detection range, and accurate and reliable results; the detection method is simple to operate, does not require nucleic acid extraction of the oriental fruit moth, has a short detection time, and the detection results are visualizable. The present invention can provide technical support for the resistance monitoring and early warning of the codling moth in the field, and also provide theoretical support for the integrated control of the oriental fruit moth.

A fertilization and maintenance device for damaged bark relates to the technical field of greening and maintenance and comprises a sealing tape and a fertilizer bag, the sealing tape is arranged on the fertilizer bag, a fertilization base layer and a fixing frame are arranged on one side face of the sealing tape, the fixing frame is located on the periphery of the fertilization base layer, a pasting plate is arranged on the fixing frame, and the fertilization base layer is communicated with the fertilizer bag; the device is easy to operate, high in practicability and very convenient to maintain, meanwhile, the device can be matched with greening equipment for cross operation, nutrients needed by trees can be effectively conveyed to the trees for growth of the trees, the larvae of the codling moths can be trapped, and the survival rate of the codling moths is increased. And biological water-logged compost is carried out by using the codling moth larvae and other similar pests, so that the function of feeding back to trees is achieved.

The present application relates to the technical field of biological detection, and a double-path apple codling moth identification system integrating RPA-LFS rapid screening and TaqMan qPCR real-time quantification, a locked nucleic acid probe primer set, a kit and a method thereof. COI Through gene screening and primer screening, the present application designs recombinase-mediated isothermal amplification (RPA) and TaqMan qPCR primers and probes for the barcode of the cytochrome oxidase subunit I (COI) gene of apple codling moth, and establishes an RPA-LFS method in combination with immunolateral flow. The RPA-LFS method can complete amplification and visual judgment within 20 minutes at 39 DEG C, and is suitable for on-site rapid detection. The TaqMan qPCR method can realize high-sensitivity quantitative analysis under the condition of crude extract. Through double-path complementary design, the consistency of the results of on-site screening and laboratory confirmation detection is realized, and the method is simple to operate and suitable for pest quarantine at ports, epidemic monitoring in agricultural production areas and quarantine at storage and transportation links.

The invention discloses a granule virus microcapsule as well as a preparation method and application thereof, a plutella xylostella granule virus or codling moth granule virus is taken as a capsule core, polydopamine is taken as a wall material, a thermal protective agent is added, and the granule virus microcapsule is prepared by a spray drying method. According to the microcapsule insecticide for the plutella xylostella granulosis virus and the codling moth granulosis virus, provided by the invention, the viral granulosis is successfully wrapped by a wall material and a thermal protective agent maltodextrin, and the adhesive force on a glass slide and a plant leaf is remarkably enhanced; the plutella xylostella granulosis virus and codling moth granulosis virus microcapsule provided by the invention can effectively improve the anti-ultraviolet capability of a viral insecticide.

The invention relates to the field of chemical synthesis of insect sex pheromones, in particular to a preparation method of codling moth sex pheromones. The method comprises the following steps: in N2 atmosphere, taking anhydrous THF as a solvent, reacting 7-chloroheptyltrimethylsilyl ether with sodium acetylene to prepare an intermediate (I), and reacting the intermediate (I) with halide salt and propionaldehyde to generate an intermediate (II); and reducing the intermediate (II) by using LiAlH4 / anhydrous AlCl3, and carrying out acid hydrolysis to obtain the codling moth sex pheromone (8E, 10E)-dodecadiene-1-alcohol. The method disclosed by the invention is mild in reaction condition, simple in post-treatment, environment-friendly, high in product yield and purity and suitable for industrial production.

The invention discloses a multi-input gating structure model (TC-MIGSM for short) based on tree coding, which is used for the risk tracing of biological risk factors of codling moths. According to the method, risk source dealers are traced through a multi-input gating structure (MIGSM for short, and the problem of data structural deficiency caused by equipment limitation in a codling moth circulation chain is solved. The traditional interpolation method cannot process the deficiency, so that the invention provides a tree coding algorithm to extract missing data features and effectively cope with the structural deficiency. Besides, internal and mutual characteristics of each dealer in the same circulation link are extracted through an auto encoder model (AE for short) and are input into the MIGSM, so that accurate risk traceability is realized. An experiment proves that TC-MIGSM is superior to other models in accuracy by tracing 208 batches of apples transported from Gansu to Beijing.

The invention discloses specific primers and a method for rapidly identifying leaf miners, and belongs to the technical field of molecular biology. According to the method, rapid identification of leaf miners is realized mainly by designing specific primers. The primer disclosed by the invention has high specificity, only performs specific amplification on the COI gene of the leaf miner, and does not have an amplification product on sibling species such as Lithocolletis ringoniella, Grapholitha molesta, Ampelopsis molesta, codling moth and Phytophorus nigricans. The primer is suitable for detecting different development stages (first-instar larvae, second-instar larvae, third-instar larvae, pupae and adults) of leaf miners and different tissue parts (tentacles, head chest, abdomen, wings and feet) of the adults, the annealing temperature range is wide, and the sensitivity is high. The method is easy and convenient to operate, the detection time is short (2-3 hours), the method can be widely applied to port quarantine, field monitoring and other scenes, and technical support is provided for precise prevention and control of leaf miners.

The invention belongs to the technical field of insect investigation, and particularly relates to a portable worm egg collecting device and method for investigation and collection. The device is mainly formed by butting two combined functional boxes through fasteners, each combined functional box is formed by clamping a bottom box, a partition plate, a middle box and a top box from bottom to top, an egg collecting system is installed in each middle box, and each egg collecting system is mainly composed of a lead screw motor, a lead screw, a clamping frame and an egg collecting block. The lead screw motor is controlled to drive the lead screw to rotate in the forward direction, and the lead screw drives the clamping frame on the bottom layer and the egg collecting blocks on the clamping frame to move downwards till the clamping frame on the bottom layer and the egg collecting blocks on the clamping frame are separated from the lead screw, fall into the bottom box through the partition plate and are taken away regularly. In this way, the requirement for collecting the worm eggs of trunk borers represented by longhorn beetles, weevils and codling moths is met; the preservation conditions that the temperature is 12-15 DEG C and the RH is 75% can be maintained in real time, and the sample activity is ensured to exceed 72 hours.

The invention relates to the technical field of agricultural pest repellent agents, in particular to a codling moth oviposition repellent agent and application thereof. The codling moth oviposition repellent comprises an active component, wherein the active component is selected from one or more of palmitic acid and linoleic acid. The repellent provided by the invention has a good oviposition repellent effect on female insects, and has a good development prospect and application value in the aspect of prevention and control of codling moths.

The invention discloses a rapid detection method for codling moths based on combination of RPA and MIRA-LFD. The invention belongs to the technical field of biology, and particularly relates to a rapid detection method for codling moths based on combination of RPA and MIRA-LFD. The composition for identification or auxiliary identification of codling moths is a composition A or a composition B, wherein the composition A consists of RPA-F1 and RPA-R1; the composition B is composed of RPA-F1, RPA-R2 and a specific fluorescent probe sequence LFD; the RPA-F1 is DNA (deoxyribonucleic acid) with a nucleotide sequence shown as SEQ ID No. 1; the RPA-R1 is DNA (deoxyribonucleic acid) of which the nucleotide sequence is SEQ ID No.2; the RPA-R2 is DNA (deoxyribonucleic acid) of which the nucleotide sequence is SEQ ID No.3; the sequence LFD of the probe is DNA with the nucleotide sequence of SEQ ID No.4.

The invention discloses an insect oviposition trapping device and method for prevention and control, and the trapping device comprises a wool felt (1) which is compressed into a corrugated shape, a group of bristles (2) which are sewed at the two ends of the wool felt (1) respectively, a supporting rod (3) and a plastic film (4). The egg laying trapping device induces codling moths to lay eggs in the device, concentratedly destroys the eggs and trapped adults, has the characteristics of simple structure and recyclability, can reduce the use of chemical pesticides, ensures the food safety and quality, and reduces the prevention and control cost while controlling the occurrence amount of pests.

The invention discloses a primer set, an identification method and an identification kit for codling moth based on the LAMP method, and belongs to the technical field of agricultural pest identification. The primer set for codling moth identification based on the LAMP method of the invention is designed according to the nucleic acid sequence shown in SEQ ID NO: 1; the primer set is used to perform a LAMP reaction with the whole genome DNA or part of the tissue of the insect to be tested as a template; after the reaction is completed, whether there is an amplification product; if there is amplification, the insect to be tested is codling moth. The invention uses a rapid molecular detection plus a visualization method to identify codling moth, and obtains the detection result within 1 hour. The visualization detection method without opening the cover can effectively avoid aerosol pollution, can accurately develop color, and the color development result is visible to the naked eye, does not require expensive instruments and equipment, and is suitable for rapid identification of codling moth on site and at the grassroots level.

The present invention relates to a disorienting composition containing trans-8-dodecene-2,2,3,3,3-pentafluoropropionate and its application. The disorienting composition of the present invention comprises a first component and a second component, wherein the first component comprises trans-8-dodecene-2,2,3,3,3-pentafluoropropionate, trans-8-dodecene-acetate, and cis-8-dodecene-1-ol; and the second component comprises trans-8-trans-10-dodecadiene-1-ol. The disorienting composition of the present invention has an excellent disorienting effect on pear borer and / or codling moth.

The invention discloses an intelligent codling moth monitoring system based on multi-source trapping data fusion, relates to the technical field of trapping data monitoring, and is used for solving the problems that monitoring strategy adjustment lags behind, and the probability of missing the optimal window period of pest prevention and control is increased. The image sensor collects and identifies the moth trapping images of the trapping points, the trapping points are screened and marked according to the trapping efficiency, the marked trapping points and the trapping growth rates of the marked trapping points are input into the space diagnosis module, the space weight is calculated in combination with the distance between the marked trapping points and neighborhood points, the space difference of the trapping growth rates is analyzed, and the moth trapping efficiency is improved. An active feature module determines transpiration intensity and environmental noise intensity of fruit trees in a detection period of the moth moth active period according to abnormal points, generates active features, calls fruit tree density in an orchard database, evaluates an environmental influence coefficient in combination with the active features, and judges whether a trapping mode is adjusted or not; and the timeliness of insect situation abnormity response is improved.

Disclosed are synthetic peptides developed from the western flower thrip (Frankliniella occidentalis), spotted-wing drosophila (Drosophila suzukii), diamondback moth (Plutella xylostella), lygus (Lygus Hesperus), and other insect neuropeptides. Peptides disclosed can be used to repel, control, or deter Frankliniella spp., Drosophila spp., Plutella ssp., and Lygus ssp., including Frankliniella occidentalis, Drosophila suzukii, Plutellaxylostella, and Lygus Hesperus from feeding on agricultural and horticultural plants. The peptides can be combined with bait materials, or applied directly to plants or areas.

The invention relates to the technical field of insect feeding, in particular to an optimized formula and application of an artificial feed for codling moths. An optimized formula of an artificial feed for codling moths comprises 75 g of corn flour, 75 g of soybean flour, 16 g of dry apple powder, 15 g of yeast powder, 20 g of cane sugar, 3 g of ascorbic acid, 1 g of sorbic acid, 0.1 g of cholesterol, 2 g of methylparaben, 1.5-6 g of mineral salt, 1-4 g of choline chloride, 14 g of agar powder and 700 ml of water. The artificial feed for codling moths provided by the invention can significantly improve the reproductive capacity of codling moths and improve the reproductive efficiency.