8 results about "Codling moth" patented technology

The utility model discloses a trapping device for preventing insects from laying eggs, which comprises a wool felt (1) compressed into a corrugated shape, a group of bristles (2) sewed at two ends of the wool felt (1) respectively, a support rod (3) and a plastic film (4). The egg laying trapping device induces codling moths to lay eggs in the device, concentratedly destroys eggs and trapped adults, has the advantages of being simple in structure and capable of being recycled, can reduce use of chemical pesticides, guarantees food safety and quality, and reduces prevention and control cost while controlling the number of pests.

The invention relates to the technical field of agricultural biology, in particular to application of a neuropeptide gene CpILP6 in relieving diapause of codling moths. After siRNA is microinjected to silence CpILP6 in a targeted manner, the diapause state of CpILP6 is changed and tends to a normal development phenotype; the research result of the invention provides a theoretical basis for defining the regulation function of the neuropeptide gene CpILP6 in the diapause induction and maintenance process of codling moth.

The present application relates to the technical field of biological detection, and a double-path apple codling moth identification system integrating RPA-LFS rapid screening and TaqMan qPCR real-time quantification, a locked nucleic acid probe primer set, a kit and a method thereof. COI Through gene screening and primer screening, the present application designs recombinase-mediated isothermal amplification (RPA) and TaqMan qPCR primers and probes for the barcode of the cytochrome oxidase subunit I (COI) gene of apple codling moth, and establishes an RPA-LFS method in combination with immunolateral flow. The RPA-LFS method can complete amplification and visual judgment within 20 minutes at 39 DEG C, and is suitable for on-site rapid detection. The TaqMan qPCR method can realize high-sensitivity quantitative analysis under the condition of crude extract. Through double-path complementary design, the consistency of the results of on-site screening and laboratory confirmation detection is realized, and the method is simple to operate and suitable for pest quarantine at ports, epidemic monitoring in agricultural production areas and quarantine at storage and transportation links.

The invention relates to the field of chemical synthesis of insect sex pheromones, in particular to a preparation method of codling moth sex pheromones. The method comprises the following steps: in N2 atmosphere, taking anhydrous THF as a solvent, reacting 7-chloroheptyltrimethylsilyl ether with sodium acetylene to prepare an intermediate (I), and reacting the intermediate (I) with halide salt and propionaldehyde to generate an intermediate (II); and reducing the intermediate (II) by using LiAlH4 / anhydrous AlCl3, and carrying out acid hydrolysis to obtain the codling moth sex pheromone (8E, 10E)-dodecadiene-1-alcohol. The method disclosed by the invention is mild in reaction condition, simple in post-treatment, environment-friendly, high in product yield and purity and suitable for industrial production.

The invention discloses a multi-input gating structure model (TC-MIGSM for short) based on tree coding, which is used for the risk tracing of biological risk factors of codling moths. According to the method, risk source dealers are traced through a multi-input gating structure (MIGSM for short, and the problem of data structural deficiency caused by equipment limitation in a codling moth circulation chain is solved. The traditional interpolation method cannot process the deficiency, so that the invention provides a tree coding algorithm to extract missing data features and effectively cope with the structural deficiency. Besides, internal and mutual characteristics of each dealer in the same circulation link are extracted through an auto encoder model (AE for short) and are input into the MIGSM, so that accurate risk traceability is realized. An experiment proves that TC-MIGSM is superior to other models in accuracy by tracing 208 batches of apples transported from Gansu to Beijing.

The invention discloses specific primers and a method for rapidly identifying leaf miners, and belongs to the technical field of molecular biology. According to the method, rapid identification of leaf miners is realized mainly by designing specific primers. The primer disclosed by the invention has high specificity, only performs specific amplification on the COI gene of the leaf miner, and does not have an amplification product on sibling species such as Lithocolletis ringoniella, Grapholitha molesta, Ampelopsis molesta, codling moth and Phytophorus nigricans. The primer is suitable for detecting different development stages (first-instar larvae, second-instar larvae, third-instar larvae, pupae and adults) of leaf miners and different tissue parts (tentacles, head chest, abdomen, wings and feet) of the adults, the annealing temperature range is wide, and the sensitivity is high. The method is easy and convenient to operate, the detection time is short (2-3 hours), the method can be widely applied to port quarantine, field monitoring and other scenes, and technical support is provided for precise prevention and control of leaf miners.

The invention discloses a rapid detection method for codling moths based on combination of RPA and MIRA-LFD. The invention belongs to the technical field of biology, and particularly relates to a rapid detection method for codling moths based on combination of RPA and MIRA-LFD. The composition for identification or auxiliary identification of codling moths is a composition A or a composition B, wherein the composition A consists of RPA-F1 and RPA-R1; the composition B is composed of RPA-F1, RPA-R2 and a specific fluorescent probe sequence LFD; the RPA-F1 is DNA (deoxyribonucleic acid) with a nucleotide sequence shown as SEQ ID No. 1; the RPA-R1 is DNA (deoxyribonucleic acid) of which the nucleotide sequence is SEQ ID No.2; the RPA-R2 is DNA (deoxyribonucleic acid) of which the nucleotide sequence is SEQ ID No.3; the sequence LFD of the probe is DNA with the nucleotide sequence of SEQ ID No.4.

Disclosed are synthetic peptides developed from the western flower thrip (Frankliniella occidentalis), spotted-wing drosophila (Drosophila suzukii), diamondback moth (Plutella xylostella), lygus (Lygus Hesperus), and other insect neuropeptides. Peptides disclosed can be used to repel, control, or deter Frankliniella spp., Drosophila spp., Plutella ssp., and Lygus ssp., including Frankliniella occidentalis, Drosophila suzukii, Plutellaxylostella, and Lygus Hesperus from feeding on agricultural and horticultural plants. The peptides can be combined with bait materials, or applied directly to plants or areas.