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Green muscardine fungus strain with high pathogenicity to codling moth and application thereof

A technology for codling moth and Metarhizium anisopliae, which is applied to Metarhizium anisopliae and its application field, can solve the problems of limiting the biological control of codling moth, lack of biological control strains, etc., and achieves good development potential and application value, and causes disease Powerful and effective in solving pesticide residues

Inactive Publication Date: 2017-04-26
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of limiting the biological control of codling moth due to the lack of good biocontrol strains, the primary purpose of the present invention is to provide a strain of Metarhizium anisopliae with high pathogenicity to codling moth

Method used

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  • Green muscardine fungus strain with high pathogenicity to codling moth and application thereof
  • Green muscardine fungus strain with high pathogenicity to codling moth and application thereof
  • Green muscardine fungus strain with high pathogenicity to codling moth and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Identification of Metarhizium anisopliae MaQLU1

[0021] The separation method adopted this time is the zombie separation method. The codling moth worms collected from Changqing Scientific Research Base of Agricultural Sciences, Shandong Province were placed in a sterilized petri dish, and the surface was sterilized by 0.1% mercury liter alcohol (dissolve 1g mercury liter in 1000ml of 95% alcohol) for 3 to 4 minutes. , and then washed three times with sterile water, placed the sterilized zombies in the center of the culture medium on the PPDA plate, cultured in a constant temperature incubator at 26°C for 10-14 days, and gently shook off the green powder on the body surface Put it into a conidia flask filled with 0.1% Tween-80 aqueous solution, shake vigorously with a magnetic stirring bar to separate the conidia of Metarhizium anisopliae individually. Gradiently dilute the spore suspension, use a pipette gun to draw a small amount of bacterial liquid and dro...

Embodiment 2

[0034] Example 2 The biological characteristics of Metarhizium anisopliae MaQLU1

[0035] 2.1 Determination of vegetative growth rate

[0036] Spread Metarhizium anisopliae MaQLU1 on the cellophane-attached PPDA plate medium by the ten-fold dilution method, and culture it in the incubator at 25-28°C and 12:12 (L:D) h, and use the cross method every day. The diameter of the colony was measured once, and three replicates were set for each treatment, and a total of 15 days were observed.

[0037] Table 1 Determination of growth rate of Metarhizium anisopliae MaQLU1

[0038]

[0039] It can be seen from Table 1 that the growth of the colony of Metarhizium anisopliae MaQLU1 is relatively fast, and the average diameter of the colony after 9 days is 36 mm, and the diameter of the colony reaches 70 mm after 15 days.

[0040] 2.2 Determination of spore production

[0041] Spread 100 μl of the cultured conidia liquid on PPDA, and after culturing for 3 days at 25°C and 12:12(L:D) h...

Embodiment 3

[0050] Example 3 Control effect of Metarhizium anisopliae MaQLU1 on codling moth

[0051] 3.1 Preparation of spore suspension

[0052] Put the isolated and purified single-spore Metarhizium anisopliae strains on PPDA medium for 12-15 days, let it grow a large number of conidia, and use a sterilized stainless steel key to scrape the cultivated conidia into a sterile In the centrifuge tube of the 100ml that fills the 0.1% Tween-80 sterile water of sterilizing, fully shake and mix on the fast shaker, count the spore suspension ( The suspension is the number of spores in Metarhizium anisopliae MaQLU1 inoculum), and the concentration of conidia is calculated according to the formula, and then the stock solution is diluted according to a certain ratio to prepare a concentration of 1 × 10 8 pcs / ml, spare.

[0053] 3.2 Test insect inoculation

[0054] The pathogenicity of this test is determined by using the dipping method, according to the above preparation of 1 × 10 8 Dip 1-year...

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Abstract

The invention discloses a strain of beauveria bassiana with high pathogenicity to scarabaeform larvas and application thereof. The provided beauveria bassiana strain is named after Beauveria bassiana BbQLU1 and is preserved at China General Microbiological Culture Collection Center, CGMCC in November 30 2015, and the preservation number is CGMCC NO.11618. By morphology and DNA-ITS sequence determination to the fungus strain, the fungus strain is determined as Beauveria bassiana. The fungus strain is fast in growth speed and strong in sporulation capacity, meanwhile can prevent and treat the scarabaeform larvas well, and has good development potential and production value.

Description

technical field [0001] The invention belongs to the field of pest biological control, relates to a strain of Metarhizium anisopliae and its application, in particular to a strain of Metarhizium anisopliae with high pathogenicity to codling moth and its application. Background technique [0002] Codling moth (Cydia pomonella), belonging to the family Lepidoptera, is an omnivorous borer pest with strong adaptability, stress resistance and reproductive ability, and is an important quarantine pest of pome fruits worldwide. Since the insect was introduced into Xinjiang, Gansu and other regions in the mid-1980s, it has caused serious damage in the western section of the Hexi Corridor and continued to spread eastward. The fruit moth rate of codling moth is generally more than 50%, and it can reach 70%-100% seriously, which seriously affects the production and sales of fruits at home and abroad. [0003] At present, the control measures against codling moth mainly include the follo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 李娇娇魏晓宇王少杰邱磊李敬龙
Owner QILU UNIV OF TECH
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