35 results about "Correlational study" patented technology

A system and method for targeting relevant research activity for clinical application in response to diagnostic markers analyses is described. Diagnostic analysis is performed to detect the level of each of at least three diagnostic markers. The levels of the tested markers are used to identify relevant publications from among a large database of articles. The most relevant literature, such as, one which reports research and studies that have been conducted to identify, moderate, and define the mechanisms unique to individual and combinations of diagnostic markers for various disease states, is then provided to the patient and/or the patient's physician, optionally with a summarization of the treatment recommendations from the provided literature. The customized information delivery provides a range of published peer-reviewed therapeutic options and/or published research studies.

The invention discloses an electroencephalogram signal processing method based on a DIVA model. According to the electroencephalogram signal processing method, fMIR data generated through the DIVA model in a simulating mode are used for carrying out positioning analysis on electroencephalogram signals, the complexity of actual computing is simplified through an independent component analysis method, and the defects that non-intrusive electroencephalogram signals are low in resolution ratio and high in interference are overcome. The fMIR data generated through the DIVA model are used for carrying out fusion processing on electroencephalogram data, and the problems that the electroencephalogram signals are low in space resolution ratio, high in signal interference and low in signal-noise ratio are resolved greatly. Through the pre-processing of ICA, the computing complexity is reduced, and the sensitivity of an equivalent dipole positioning algorithm to the noise is overcome greatly. Finally, the electroencephalogram signal processing method is used for processing actual experiment data, and the obtained conclusion meets the physiology reality. A feasible resolution scheme is provided for the electroencephalogram signal processing problem in a Chinese nerve analysis system, and a foundation is laid for the research related to generation and obtaining of Chinese phonetic symbols in future.

The invention discloses a physical property measurement system and a method for a material based on an AFM (atomic force microscope) probe force curve. According to the method, a high-speed and high-precision digital acquisition card and a digital controller are adopted to realize broad-band and high-resolution measurement during a probe force curve testing process. Therefore, a peak force in the probe force curve can be controlled and measured precisely. On the basis of acquiring the force curve, a Hertz contact model and a Derjaguin-Muller-Toporov (DMT) model are used for acquiring appearance information of the surface of a sample, and a power dissipation and force curve area integral method is used for acquiring a viscous force and dissipated energy information of the sample. According to the system and the method, a large amount of information reflecting physical properties of the surface of the sample is included. The application scope of an AFM is expanded greatly, the development of correlational study fields is promoted, and the system and the method have very important scientific values and good application prospects.

The present invention relates to systems and methods for real time diagnostic and data correlation utilizing a computer network. One embodiment of the present invention relates to a method for interfacing a health monitoring system with an on-line medical information site to provide real-time diagnostics and or updated research data correlation. The method includes measuring and recording at least two health related values, compiling the at least two health related values into a format consistent with an online medical information database, and transmitting the compiled versions of the at least two health related values to an online medical information database. The method may further include receiving real time diagnostics from a medical professional. The method may also include receiving updated medical research data corresponding to the transmitted values. An alternative embodiment of the present invention relates to a system for providing real time remote diagnostic and correlated research data. The system includes a medical monitoring system configured to measure at least two health related values, a remote medical database which is accessible by diagnostic medical professionals and which contains updated medical research data, and a communication link between the medical monitoring system and the remote medical database.

The invention discloses an EST-SSR primer group developed on the basis of transcriptome sequences of hemarthria compressa and hemarthria altissima. The EST-SSR primer group comprises 52 pairs of primers, and nucleotide sequences of the primers are represented as sequence tables SEQUENCE ID NO.1-104. The invention further relates to an application of the EST-SSR labeled primers in the aspects of genetic diversity analysis of hemarthria sibirica and transferability of the hemarthria sibirica in gramineae species. The 52 pairs of primers in the EST-SSR primer group developed on the basis of the transcriptome sequences of hemarthria compressa and hemarthria altissima have the advantages of abundant polymorphism, stable amplification, good repeatability, convenience in counting and the like, can be effectively applied to research fields such as hemarthria sibirica germ resource genetic diversity analysis, variety identification, fingerprinting construction, molecular marker assisted breeding and the like and are applied to correlational research of other gramineae forage species.

The invention discloses a brown planthopper VgR polypeptide and multi-resistant preparation method thereof. The amino acid sequence of VRG polypeptide is RKGNADQSVATKSD, the preparation method for polyclonal antibody comprises the following steps: (1) performing epitope analysis on brown planthopper VRG protein antigen; (2) designing and analyzing brown planthopper VgR; (3) crosslinking synthetic peptide and carrier protein; (4) preparing rabbit antibody brown planthopper VgR polypeptide antibody; (5) collecting, separating to obtain serum containing antibody, purifying antibody to obtain the antibody of brown planthopper VgR polypeptide. The brown planthopper VgR polypeptide polyclonal antibody is good in specificity, and high in purity, has specific binding reaction with the brown planthopper VgR protein and is used for performing correlation research for brown planthopper VgR protein, such as analysis of character, function, expression profile and content.

The invention relates to a preparation method of monoclonal protein of mice, in particular to preparation and application of IL-15 isoform protein of mice. In the preparation method, the IL-15 isoform protein of the mice is prepared in the excitation state of an immunity system, the antagonistic effect of the IL-15 isoform protein on IL-15 protein of the normal mice is detected through in-vitro experiments, and the application of the isoform protein in clinical treatment such as autoimmune diseases and the like is provided. The study proves that pulpar cells which are not stimulated by LPS cannot detect the isoform, but the isoform can be detected in macrophage and B cells which are stimulated by the LPS and are from primary culture or cell strains, and the isoform is possible to have important negative feedback regulate and control effect under the condition that the immune system is activated. In the preparation method, the isoform protein is discovered under the condition that the immune system is activated for the first time, and is subjected to correlational studies of the functions thereof, and thus a more direct evidence is provided for the effect of the isoform protein in the regulation and control of organism immunity.

The invention discloses an indoor artificial breeding method for codling moths. The growth process of the codling moths is 'eggs-larvas-pupas-imagoes', the imagoes mate to lay the eggs, and populationbreeding is achieved. The indoor artificial breeding method comprises the steps that indoor space suitable for the growth of the codling moths with controllable temperature, humidity, illumination and other environments is established; apples with worms are collected from the wild to acquire pest sources of the codling moths, the worms are taken back for breeding to form subcultured indoor codling moth population. According to the indoor artificial breeding method for the codling moths, the breeding operation method is simple, the breeding environment is controlled easily, the long-term breeding can establish the stable indoor codling moth population, provide adequate pest source worms for the subsequent correlational studies on the codling moths, and provides conditions for the prevention and cure research of the codling moths.

The invention relates to a method for measuring the stability of a membrane protein in a lipidic cubic sample through spectroscopy. The method comprises the following steps of: uniformly mixing a purified target protein into the lipidic cubic sample, and then carrying out a gradient heating/cooling process with temperature increased step by step on the lipidic cubic sample; cooling the lipidic cubic sample again and centrifugalizing the lipidic cubic sample at a high speed to ensure that water molecules enter the lipidic cubic sample again and the lipidic cubic sample is at a colorless and transparent state again when the lipidic cubic sample becomes turbid as water molecules are separated out of the lipidic cubic sample at every step of temperature rise; and then identifying the stability of the target protein by acquiring fluorescent signals of the lipidic cubic sample. The method disclosed by the invention is reasonable in design and high in operability, can realize that the stability of the protein can be directly measured in the lipidic cubic sample, and provides the data and theory support for subsequent crystal and correlational research; and a gene applied to the method disclosed by the invention can also provide the support for control research on various classified micromolecules.

The invention relates to a method for separating and expanding cardiac microvascular endothelial cells of adult rat vitro, which provides an ideal cell model for the correlational studies of cardiac endothelial function and a complementary source of vascular neogenesis treatment and tissue engineering cells after myocardial infarction, and lays foundation for deeply studying the clinical treatment of myocardial infarction, angiocarpy endothelial dysfunction, etc. The method adopts the technical proposal that (1) by utilizing the biological characteristics of the cardiac microvascular endothelial cells, the cardiac microvascular endothelial cells of the adult rat are separated and enriched by adopting a method of enzymic digestion, and are expanded by utilizing the self-renewing and proliferation characteristics of the endothelial cells, thus obtaining a great deal of cardiac microvascular endothelial cells; (2) the separated cells are separated and purified vitro; (3) the separated cells are purified and expanded vitro.

The invention discloses a lentiviral vector of fusion green fluorescent protein with over expression of RAB7A and EGFP genes, a building method and application thereof. The vector takes a lentiviral vector LV11 as a skeleton vector, wherein an EGFP gene segments and RAB7A gene segments are inserted behind a CMV promoter of the vector LV11 in a direction from 5' to 3'. By virtue of the lentiviral vector of fusion green fluorescent protein with over expression of RAB7A and EGFP genes, the problem that over-expression vector is effective and intuitive in the study of RAB7A genes can be solved. The vector takes CMV as the promoter, so that the high-efficiency expression of eukaryotic gene can be ensured; EGFP is used as a marker, the autophagy and endocytosis degree of cells and the effect of RAB7A genes in cell hypoxia process can be judged by intuitively observing the intensity of the expressed fluorescence; a good tool is provided for studying the functions of the RAB7A genes; the basis is provided for the subsequent correlation studies of RAB7A.

The invention discloses a method for analyzing the content of Abeta plaque imaging agent precursor AV-45-OTS through micellar electrokinetic chromatography and belongs to the technical field of analytical chemistry. The content of the AV-45-OTS can be easily and conveniently measured through a capillary electrophoresis method. An effective analysis means is provided for controlling the quality of a drug box, which is not reported in correlational research. 1 mg of the AV-45-OTS is precisely weighed and dissolved in 0.5 mL of chromatographic pure tetrahydrofuran and 1.5 ml of 25 mmol/L borax-10 mmol/L SDS solution, sample injection at the voltage of 10 kv is performed, the voltage is kept unchanged, the sample injection time is changed, linear regression is performed on the sample injection time at the peak area, the linear relation is good when the sample injection time is 8-16 s, the regression equation is y=112958x-525878, and the correlation coefficient r2 is equal to 0.9909. Compared with HPLC, the micellar electrokinetic chromatography has the main advantages that separation efficiency is high, the efficiency can be 500,000 theoretical plate number/m and is ten times that of the HPLC; the micellar electrokinetic chromatography has other advantages that speed is high, the quantity of samples in use is small, consumption of reagents is small, and running cost can be reduced.

The invention discloses an approximate evaluation method for uncertain time-delay on the basis of an H-infinity principle. The approximate evaluation method comprises the following steps: constructinga mathematical model of stable performance indexes of a feedback control system on the basis of the H-infinity principle and a mathematical model of a feedback control system with uncertain time-delay; analyzing influence of the uncertain time-delay to the stable performance indexes of the feedback control system, and scaling the mathematical model of the stable performance indexes into a feedback control system stabilizing condition with time delay correlation term; and based on a Pade approximation method, constructing a rational approximate function related to the time delay correlation term, setting a target constraining condition for the rational approximate function capable of replacing the time delay correlation term, and replacing the time delay correlation term by using the rational approximate function which meets a target constraint condition so as to obtain the stable performance indexes of the feedback control system. By the method, a rational estimation function which isefficient and has high degree of fitting can be provided; and the method can be widely applied to the feedback control system with uncertain time-delay and engineering related research problems.

The invention discloses a spatial position data processing method and system. Firstly, quality evaluation is performed on sparse sampled spatial position big data on space-time sampling; therefore, the method starts from the basic characteristic of inherent space-time distribution in individual position big data of sparse sampling; through dynamic period division, computer random simulation and calculation and regression analysis, a quantitative loss evaluation model for describing typical characteristics of urban resident activities by aiming at sparse sampling spatial position big data is provided, and quality loss distribution characteristics corresponding to different sampling rates are clearly explained. Finally, the sample data set meeting the actual requirements is selected according to the distribution of the obtained deviations, so that the conclusion of related research and analysis is more reliable, and the actual application is guided more scientifically.

The invention belongs to the field of plant genetic engineering, particularly relates to an application of fast period rape to the field of research on rape functional genomics, and further disclosesa method for editing a brassica napus L. functional gene based on the fast period brassica napus L. and a method for preparing a mutant by chemical mutagenesis. The scheme is correlational research oncorrelational research on the rape functional gene based on the fast period rape, and specially comprises the steps of editing the rape gene and/or preparing the rape gene mutant. Based on the advantages of being short in growth period and small in plants, the brassica napus L. functional gene can replace arabidopsis thaliana to realize research on the rape gene within a short period.

The invention relates to a separation and purification method of crosslinked tetrahymena micronucleus. The separation and purification method of the crosslinked tetrahymena micronucleus comprises the following specific steps of performing crosslinking treatment on tetrahymena cells by using crosslinking agents such as formaldehyde; splitting the cells by using chemical cleavage; and finally separating micronucleus of the tetrahymena in a centrifugal mode. Owing to the separation and purification method of the crosslinked tetrahymena micronucleus, Hi-C and correlational studies in tetrahmena thermophila are feasible.

The invention relates to a chromosome and karyotype analysis method taking pitaya stem tips as materials. Specifically, the young and tender stem tips of the pitaya are used as experimental materials, and chromosome morphology observation and research are carried out by utilizing the characteristic that the young and tender stem tips have meristematic region tissue cells with exuberant division. The method comprises the following steps that healthy and tender pitaya stem tips are cut off and then dissected; putting the dissected stem tips into a mixed solution of colchicine and 8-hydroxyquinoline, and carrying out chromosome concentration; soaking the stem tips with KCl; the stem tips are refrigerated and fixed with stationary liquid; cleaning the fixed root tips, and dissociating the root tips in HCl; the dissociated stem tips are cleaned and put into a mixed solution of cellulase and pectinase to be subjected to enzymolysis; cleaning the stem tips subjected to enzymolysis, and putting the cleaned stem tips into double distilled water for post-hypotonic treatment; putting the post-hypotonic stem tips into a stationary liquid for refrigeration treatment; and placing the stem tip on a glass slide, cutting, dyeing, and preparing a sheet for microscopic examination. The problems that the aerial roots of the pitaya are difficult to draw, the root tips of the pitaya are difficult to draw, the plants are damaged, and the experimental effect is unstable are solved. According to the method, the pitaya stem tips serve as materials for the first time, karyotype analysis of the pitaya chromosomes is achieved, and more convenient and stable technical support is provided for correlational researches such as karyotype research of the pitaya chromosomes and ploidy identification of germplasm resources.

The invention provides an extrusion casting process parameter design method of an improved KNN algorithm based on a similarity principle, and belongs to the technical field of process parameter design, and the process parameter design method comprises the following steps: firstly, measuring the relative change trend of related variables of process parameters based on the similarity principle; on the basis, sample data similar to the relative change trend of related variables of the target case are extracted, KNN clustering is carried out based on the sample data, and weighted regression is carried out on corresponding parameters in clustered samples to serve as technological parameter design values of the target case. According to the design method, based on existing research data, existing related research results are fully referred and utilized, tedious experimental research is not needed, and the design precision of technological parameters is high. Compared with an existing process parameter design method, a large amount of time cost and experiment cost are saved. The method has universality, and is not only suitable for design of extrusion casting process parameters, but also suitable for parameter design in other similar fields.

The invention discloses a doctor-patient trust construction system and method based on a four-quadrant model, relates to the field of four-quadrant model analysis, and aims to solve the problems that an existing doctor-patient trust construction system and method are one-sided and poor in difference adjustment information accuracy. According to the scheme, the system comprises a research method determination module, a research result analysis module and a summarization module, the research method determination module is connected with the research result analysis module, and the research result analysis module is connected with the summarization module. According to the method, the total Cronback's alpha reliability coefficient of the scale is 0.912; the bisection reliability R is 0.863 and 0.932; the content validity Pearson correlation coefficient ranges from 0.38 to 0.81, statistical significance is achieved, the structural validity RMR is 0.065, the RMSEA is 0.073, the x2/df is 2.236, the GFI is 0.791, and a'four-quadrant model 'doctor-patient trust measurement scale has good reliability and validity and can be used in relevant research of doctor-patient trust in China.

The invention discloses an SNP (single nucleotide polymorphism) marker for geospecific biomphalaria tracing and a detection method and application of the SNP marker. The SNP marker comprises at leastone of three SNP molecular marker bodies, namely SNPs of the 501st position in the SEQ ID NO:1, the 501st position in the SEQ ID NO:2 or the 501st position in the SEQ ID NO:3. The detection method includes: extracting a biomphalaria sample DNA; performing PCR (polymerase chain reaction) amplification and single base extension on target genes of sample DNA to form sample analytes; performing cocrystallization of sample analytes and matrixes of mass spectrometry chips and detecting the SNPs of the target genes to achieve precise typing of the different SNPs. By the above method, SNP typing information of the different positions can be acquired rapidly and accurately, and reliable reference information is provided for subsequent correlation research. The biomphalaria from the different areascan be distinguished according to the SNP chips of the positions, and rapid tracing detection is realized.

The invention relates to the field of data mining in bioinformatics, in particular to a synthetic lethal interaction prediction method based on a heterogeneous graph convolutional neural network. The method mainly comprises the steps of (1) collecting breast cancer FPKMRNA-seq information, breast cancer CNV data and breast cancer high-confidence risk gene information; (2) preprocessing breast cancer copy number variation (CNV) and mRNA data by a rank-based method; (3) establishing a gene-gene interaction network based on protein interaction knowledge and a signal channel, and generating a high-density sub-network by using a network clustering algorithm; and (4) constructing a group sparse learning-based model to describe the incidence relation between the subnet and the mRNA expression of the target gene, measuring the ability of the CNVs to predict the expression change of the target gene by using a root mean square error (RMSE), carrying out cross validation by adopting a k-fold cross validation algorithm, and carrying out correlation analysis on the gene expression and the CNV by using a Sperman correlation research method in combination with pathway enrichment analysis.

The invention discloses a pig IGFN1 protein polyclonal antibody, further discloses antigenic polypeptide for inducing the pig IGFN1 protein polyclonal antibody and belongs to the field of animal gene engineering. The antigenic polypeptide is formed by the amino acid sequence as shown by SEQ ID No: 1. The nucleotide sequence shown by SEQ ID No: 2 forms the antigenic polypeptide encoding genes. The pig IGFN1 protein polyclonal antibody is high in pig IGFN1 protein expression level detection specificity, and an important tool is provided for expression analysis and correlational studies of IGFN1 in pig tissues. The antigen sequence is long, and the detection sensitivity is high.