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SNP (single nucleotide polymorphism) marker for geospecific biomphalaria tracing and detection method and application of SNP marker

A kind of double umbilical snail, specific technology, applied in the field of SNP labeling and detection of geographically specific double umbilical snail traceability, can solve the problem of unseen and other problems, and achieve the effects of simple operation, improved operability, and strong reliability.

Active Publication Date: 2019-06-21
SUN YAT SEN UNIV
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Problems solved by technology

Therefore, providing an efficient, fast, sensitive, and accurate SNP detection method will provide important reference information and theoretical support for the establishment of geographically specific SNP molecular markers for tracing the origin of the snail and the establishment of a traceability detection system. See SNP molecular markers or SNP chips for geographically specific traceability of double umbilical snails

Method used

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  • SNP (single nucleotide polymorphism) marker for geospecific biomphalaria tracing and detection method and application of SNP marker
  • SNP (single nucleotide polymorphism) marker for geospecific biomphalaria tracing and detection method and application of SNP marker
  • SNP (single nucleotide polymorphism) marker for geospecific biomphalaria tracing and detection method and application of SNP marker

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Experimental program
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Embodiment 1

[0051] 1. Sample source

[0052] 360 double-umbilical snails were collected from Shenzhen, Guangdong (170), Dongguan, Guangdong (109), and Hong Kong (81).

[0053] 2. DNA extraction

[0054] Genomic DNA was extracted from the samples of the umbilical snail, quantified by a spectrophotometer, and checked by agarose gel electrophoresis. Genomic DNA concentration>10ng / μL, OD 260 / OD 280 >1.7, take samples with bright and clear electrophoresis strips without drag for subsequent operations. The sample DNA that passed the quality inspection was diluted with deionized water to a concentration of 20 ng / μL, transferred to a 384-well plate, and stored at -20°C for later use.

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Abstract

The invention discloses an SNP (single nucleotide polymorphism) marker for geospecific biomphalaria tracing and a detection method and application of the SNP marker. The SNP marker comprises at leastone of three SNP molecular marker bodies, namely SNPs of the 501st position in the SEQ ID NO:1, the 501st position in the SEQ ID NO:2 or the 501st position in the SEQ ID NO:3. The detection method includes: extracting a biomphalaria sample DNA; performing PCR (polymerase chain reaction) amplification and single base extension on target genes of sample DNA to form sample analytes; performing cocrystallization of sample analytes and matrixes of mass spectrometry chips and detecting the SNPs of the target genes to achieve precise typing of the different SNPs. By the above method, SNP typing information of the different positions can be acquired rapidly and accurately, and reliable reference information is provided for subsequent correlation research. The biomphalaria from the different areascan be distinguished according to the SNP chips of the positions, and rapid tracing detection is realized.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and more specifically relates to a SNP marker, a detection method and an application for traceability of geographically specific umbilical snails. Background technique [0002] Double-umbilical snails belong to the phylum Molluscs, Pneumonia subclass, and Snail family. Among the more than 30 known species of double-umbilical snails, double-umbilical snails smooth, double-umbilical snails, shoal double-umbilical snails, and Puer's double-umbilical snails 18 species including S. mansoni and other 18 species can act as intermediate hosts of Schistosoma mansoni to spread schistosomiasis, causing serious disease burden to the world, especially in Africa, South America, Asia and other regions, and seriously hindering social and economic development. Among them, the double umbilical snails are mainly distributed in the fresh waters of St. Lucie in the Caribbean Sea and the northeastern coast o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCY02A90/10
Inventor 吕志跃胡玥黄萍周洪利马玉斌周昱旻
Owner SUN YAT SEN UNIV
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