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57 results about "Chemical Cleavage" patented technology

Bond cleavage, or scission, is the splitting of chemical bonds. This can be generally referred to as dissociation when a molecule is cleaved into two or more fragments.

Protein simple purification method for positioning recombinant protein to surfaces of cells

The invention discloses a protein simple purification method for positioning recombinant protein to the surfaces of cells. The method is characterized by comprising the following steps: constructing an encoding signal peptide, a chemical cleavage site and a target protein gene into an expression vector, and positioning target protein to the surfaces of the cells after being expressed; and adding abuffer solution containing a cleavage reagent into the collected cells for resuspension, and performing centrifugation to obtain the supernatant, so as to obtain a target protein solution. Accordingto the protein simple purification method disclosed by the invention, the recombinant protein is secreted and positioned to the surface of a cell after being expressed in the cell by utilizing the positioning function of signal peptide, and by utilizing the advantages of intermediate chemical shearing sites of the recombinant protein, the high-purity target protein can be simply and conveniently obtained by adding a cleavage reagent into a buffer solution for resuspending cells and centrifuging. The method omits complex steps that in the prior art, protein purification needs cell lysis and chromatographic columns with various properties are used to obtain target protein, is simple to operate, and greatly saves the time and costs.
Owner:上海百英生物科技股份有限公司

Method for extracting nucleic acid from activated carbon biological membranes in drinking water treatment

InactiveCN102719425AHigh anti-corrosion abilityReduce adverse effects of extractionDNA preparationWater bathsPolyvinylpyrrolidone
The invention discloses a method for extracting nucleic acid from activated carbon biological membranes in drinking water treatment and relates to the technical field of microbiology of biological activated carbon process units in environmental engineering drinking water deep treatment. The method includes: firstly, subjecting activated carbon particles to dehumification by the aid of dehumification buffer solution; and then performing water bath and ultrasonic elution. The method specifically includes: subjecting activated carbon to dehumification by the aid of the dehumification buffer solution obtained by mixing NaOH solution or NaOH with polyvinylpyrrolidone, and extracting the microbial nucleic acid on the biological activated carbon after ultrasonic elution and chemical cleavage. The total DNA (deoxyribonucleic acid) length is larger than 20 Kb so as to completely meet the requirements of PCR (polymerase chain reaction) analysis. According to the method, the problem of difficulty in elution resulted from low biomass of the biological activated carbon and close combination of the biological activated carbon with carrier particles is solved, and adverse effect of humic acid on microbial nucleic acid extraction is weakened, so that microbial cells are sufficient in cleavage. The method is simple and convenient to operate and high in DNA output and purity.
Owner:TSINGHUA UNIV

Safe synthesis method of 1,3,5-triamino-2,4,6-trinitrobenzene

The invention provides a safe synthesis method of 1,3,5-triamino-2,4,6-trinitrobenzene, wherein the safe synthesis method comprises the steps: based on the isomerism characteristics of 1,3,5-cyclohexyltriketone imine and 1,3,5-triaminobenzene, taking 1,3,5-cyclohexyltriketone as a reaction raw material, and preparing N,N',N''-triacetyl-1,3,5-triaminobenzene through a trioximation reaction, an N-O bond cleavage-isomerism reaction and an N-acylation reaction; and taking N,N',N''-triacetyl-1,3,5-triaminobenzene as a raw material, and preparing TATB through a multi-stage one-pot tri-nitration reaction. The method has the significant characteristic of the multi-stage one-pot tri-nitration reaction, so that the amination step of an explosive multi-nitro-compound is avoided so as to substantially improve the safety, the used reactants or the used catalyst is the common product in the chemical industry, the use of a non-degradable organic solvent is avoided, the green and safe synthesis is achieved; and meanwhile, on the basis of the characteristics of a one-pot method, the operation process is simplified, operations such as separation, purification and collection of an intermediate product are avoided, and large-scale preparation of 1,3,5-triamino-2,4,6-trinitrobenzene is facilitated.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY
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