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Application of fast period rape to field of research on rape functional genomics, and method

A fast cycle, rapeseed technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of small Arabidopsis genome, difficulty in hybridization experiments, and no direct use value, so as to speed up the progress of basic research and achieve high-throughput Effects of research, saving research time and cultivating space

Pending Publication Date: 2020-10-16
珠海市农腾乐油植物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, with the development of rapeseed breeding and basic research, the limitations of using Arabidopsis as an alternative are becoming more and more obvious. This is because: (1) Arabidopsis genome is small, and there are many genes in rapeseed that are not in Arabidopsis , especially genes related to yield or heterosis utilization, such as rape dominant sterility gene MS5 (Xin Qiang et al. 2016), can only be cloned or verified when rape is used as a donor; (2) Arabidopsis flower bud ratio Rapeseed is small, so the hybridization test is difficult; (3) more importantly, Arabidopsis itself is a weed, which has no direct use value in field production practice, and its positive test products cannot be put into production practice.

Method used

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  • Application of fast period rape to field of research on rape functional genomics, and method
  • Application of fast period rape to field of research on rape functional genomics, and method
  • Application of fast period rape to field of research on rape functional genomics, and method

Examples

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Embodiment 1

[0041] In this example, fast-period rapeseed was used as a donor for Fad2 gene editing to create a new fast-period rapeseed with high oleic acid. Oleic acid is one of the main components of rapeseed oil fatty acid, which has the functions of reducing "three highs" (hyperlipidemia, hyperglycemia, and hypertension) and preventing tumors (Piccinin et al., 2019). Therefore, high oleic acid (oleic acid content> 75%) breeding is one of the important goals of rapeseed quality breeding in recent years. Oleic acid synthesis in rapeseed is controlled by the Fad2 gene (Yang et al., 2012), and when wild-type Fad2 is mutated to fad2, a high oleic acid phenotype is exhibited. In this example, taking the Fad2 gene editing process of fast-period rapeseed as an example, the Fad2 gene on the fast-period rapeseed A genome is directionally mutated into fad2, and a new fast-period rapeseed with high oleic acid is obtained.

[0042] The method for obtaining high oleic acid new fast cycle rapeseed ...

Embodiment 2

[0096] In this example, fast-period rapeseed was used as a donor to perform EMS chemical mutagenesis to obtain rapeseed gene mutants. In rapeseed functional genomics research or breeding, it is often necessary to create various mutants, physical mutagenesis (such as 60 Co-γ rays, etc.) or chemical mutagenesis (such as EMS, etc.) are commonly used treatment methods. Using traditional rapeseed, although the mutagenesis is relatively simple, the treatment of the mutagenized progeny is very troublesome, requiring a lot of land resources for field planting, limited by seasons, and it is difficult to determine whether the phenotype difference between a single plant is caused by genetic variation Or caused by environmental differences. However, if fast-cycle rape is used as a donor, due to the miniaturization of a single plant and the need for vernalization, one generation can be harvested in 60 days, so large-scale planting (that is, high-throughput planting) can be realized under ...

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Abstract

The invention belongs to the field of plant genetic engineering, particularly relates to an application of fast period rape to the field of research on rape functional genomics, and further disclosesa method for editing a brassica napus L. functional gene based on the fast period brassica napus L. and a method for preparing a mutant by chemical mutagenesis. The scheme is correlational research oncorrelational research on the rape functional gene based on the fast period rape, and specially comprises the steps of editing the rape gene and / or preparing the rape gene mutant. Based on the advantages of being short in growth period and small in plants, the brassica napus L. functional gene can replace arabidopsis thaliana to realize research on the rape gene within a short period.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, specifically relates to the application of a fast-period rapeseed in the field of functional genomics research, and further discloses a method for editing functional genes based on fast-period Brassica napus and preparing functional gene mutations by chemical mutagenesis body method. Background technique [0002] Brassica napus L. is an important oil crop widely planted in my country. It is planted in large areas in the Yangtze River Basin, Yunnan-Guizhou Plateau and Northwest China with a cool climate, with a total annual planting area of ​​about 100 million mu. Its genome is AACC, with 19 pairs of chromosomes, about 1200-1280Mb in size (Song Jia-Ming et al., 2020), and has a complex structure with many homologous fragments. Rapeseed likes to be cold and cool, and it needs a certain period of low temperature treatment to bloom (usually 4-10°C). The growth period of autumn sowing in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00A01H6/20A01H1/02A01H1/04A01H1/06
CPCC12N15/8216C12N15/8241A01H1/02A01H1/04A01H1/06
Inventor 杨梅杨铭浩邓扬菲杨峻辉孟冬林杨峻烨莫从古许兴旺钱汇海
Owner 珠海市农腾乐油植物科技有限公司
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