A special feed for artificial breeding of coffee wood beetle larvae and artificial breeding method thereof

A technology of wood beetle moth and larvae, which is applied in the field of special feed for artificial breeding of coffee wood beetle larvae, can solve problems such as indoor breeding methods of coffee wood beetle moth that have not yet been seen, and achieve the effect of shortening the pupal period

Active Publication Date: 2022-02-15
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, there is no relevant report on the indoor breeding method of coffee wood beetle moth

Method used

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  • A special feed for artificial breeding of coffee wood beetle larvae and artificial breeding method thereof
  • A special feed for artificial breeding of coffee wood beetle larvae and artificial breeding method thereof
  • A special feed for artificial breeding of coffee wood beetle larvae and artificial breeding method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Insects to be tested: From May to September 2016, 150 coffee wood beetle larvae were collected from Nanjing Liuhe Lvzhou Thin-shell Pecan Base.

[0030] 2. Feeding method

[0031] 1) Artificial feed preparation

[0032] Collect the 2- and 3-year-old branches of hickory nuts from the wild, remove the bark, cut the rest into pieces of about 1 cm, and put them into a plant grinder to grind them into sawdust. As shown in Table 1, feeds of 4 different formulations of A, B, C and D were prepared. The operation steps are: put quantitative agar powder, yeast extract, acetylcholine chloride, cholesterol, ascorbic acid, glucose, riboflavin, casein, sorbic acid and methyl p-hydroxybenzoate into a 500mL beaker, add 100mL distilled water, Use a digital display constant temperature magnetic stirrer (Shanghai Hannuo Instrument Co., Ltd.) to dissolve it; finally, according to different feed formulas, add quantitative thin-shelled hickory sawdust, soybean flour, and corn flour and ...

Embodiment 2

[0047] The artificial breeding method of coffee wood beetle larvae is the same as in Example 1, wherein two treatments are established at 24° C., darkness, feed B (treatment 1) and 30° C., darkness, feed B (treatment 2), and each treatment is repeated 47 times. Select 94 coffee leopard moth larvae with basically the same physiological state, put one in each glass test tube, and then put them into an incubator with a certain temperature, darkness, and relative humidity of 60% to 70%. The feed was changed every 7 days, the head shell width and body length of the larvae were measured and weighed, the molting, pupation and death of the larvae were closely observed, and the growth and development of each larvae were recorded in detail. The results are shown in Table 3.

[0048] Table 3 Results of different treatments

[0049] deal with processing 1 processing 2 Breeding number (head) 47 47 Number of surviving larvae (head) 42 39 Larval survival rate...

Embodiment 3

[0052] The method of artificial rearing of coffee wood beetle larvae is the same as in Example 1, wherein three treatments were set up in the test, namely treatment 1: dark, 24°C, feed B; treatment 2: indoor natural light, 24°C, feed B; treatment 3: L:D=12:12, 24°C, feed B, each treatment repeated 40 times. Select 120 coffee wood beetle larvae with basically the same physiological state, put one in each glass test tube, and then put them into an incubator at 24°C and a relative humidity of 60% to 70%. The feed was changed every 7 days, the head shell width and body length of the larvae were measured and weighed, the molting, pupation and death of the larvae were closely observed, and the growth and development of each larvae were recorded in detail. The results are shown in Table 4.

[0053] Table 4 - The results of each treatment

[0054]

[0055]

[0056] As can be seen from Table 4, the dark conditions in the light incubator are the most suitable for larvae growth, t...

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Abstract

The invention discloses a special feed for artificial feeding of coffee wood beetle larvae and an artificial feeding method thereof. The feed is composed of the following components: 50 g of hickory sawdust, 0-30 g of soybean powder, and 0-30 g of corn flour , agar powder 5.0g, yeast extract 0~2.0g, acetylcholine chloride 0.2g, cholesterol 0~0.3g, ascorbic acid 0~0.5g, glucose 0~2.5g, riboflavin 0~0.4g, casein 0~ 0.4g, 0-0.8g sorbic acid, 0-0.6g methyl p-hydroxybenzoate, 100mL distilled water, wherein 0g is different for soybean flour and corn flour. The pupation rate of coffee wood beetle larvae fed with feed B in the present invention is 73.3%, and the eclosion rate is 66.7%. It can be seen that feed B is a special feed suitable for the growth and development of larvae. The rearing experiment proved that feeding larvae with Feed B was the best at 24°C and in the dark, and putting the pupae under the condition of L:D=12:12 could shorten the pupae period.

Description

technical field [0001] The invention belongs to the technical field of insect feeding, and in particular relates to a special feed for artificial feeding of coffee wood beetle larvae and an artificial feeding method thereof. Background technique [0002] One of the artificial feed formulas designed by Lu Changkuan et al [artificial feeding of the sea buckthorn wood beetle, "Journal of Applied Entomology", 2005, 42 (2): 211-214] is: corn flour 17g, soybean powder 23.15 g, 2 g of sucrose, 2 g of yeast extract powder, 0.2 g of potassium sorbate, 0.12 g of methyl p-hydroxybenzoate, 1 g of agar, and 54.53 mL of water. The rate is 30%, which is better than the other two artificial feeds. [0003] So far, there is no relevant report on the indoor breeding method of coffee wood beetle moth. Contents of the invention [0004] Purpose of the invention: In view of the fact that there is no report on the feeding of coffee wood beetle larvae, the purpose of the present invention is t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/90A01K67/033
CPCA01K67/033A23K50/90
Inventor 巨云为曹霞严晓茹朱晨曦刘凯舟
Owner NANJING FORESTRY UNIV
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