Forage for artificial breeding of Monochamus alternatus Hope and method for preparing the same

An artificial technology of monochamus monochamus, applied in animal feed, animal feed, application, etc., can solve the problems of not being able to meet the requirements of use, sampling is limited by seasons, and the preparation process is cumbersome, so as to achieve stable feeding effect, reduce feeding time, highly operable effect

Inactive Publication Date: 2011-03-09
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In Assessment of Artificial Rearing Method for the Pine Sawyer Beetle Monochamus alternatus (Coleoptera: Cerambycidae) (Ismail Aloo, Kazumasa Katagiri.. 1994, Bull. Tokyo. Univ. For., 92: 153-166) In the study, the author selected 11 formula feeds to raise Monochamus alternatus at room temperature and constant temperature (25 oC), but these feeds either had complex ingredients and cumbersome preparation process; The indicators of the longhorn beetle are relatively poor; or it is not feasible to cut down a large amount of wood; neither can meet the use requirements
In Rearing the Japanese Pine Sawyer Monochamus alternatus (Coleoptera: Cerambycidae) on artificial diet (Hajime Kosaka, Nobuo Ogura.. 1990, Appl. Ent. Zool., 25(4): 532-534) , based on silkworm feed, adding tree inner skin, dry yeast, and distilled water to mix and prepare feed, there are also problems such as sampling is limited by season, work is cumbersome, and feed cost is high

Method used

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  • Forage for artificial breeding of Monochamus alternatus Hope and method for preparing the same
  • Forage for artificial breeding of Monochamus alternatus Hope and method for preparing the same
  • Forage for artificial breeding of Monochamus alternatus Hope and method for preparing the same

Examples

Experimental program
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Embodiment 1

[0021] The method for preparing the above-mentioned feed comprises: adding 800ml of distilled water in a cleaned container, adding 40g of agar after boiling, and adding the de-pine wood chips and H 2 SO 4 After fully heating the other components, add about 1N H 2 SO 4 Adjust the pH of the feed, then slowly add pine wood chips that have been dried and sterilized at 60°C for 8 hours and passed through a 20-mesh sieve, and fully stirred and mixed evenly. After stopping the heating, pour the liquid feed into a porcelain plate to cool naturally, seal it with a plastic wrap after it condenses into a block, put it in a refrigerator and store it at 4°C for later use.

[0022] Example 2

Embodiment 2

[0024] Example 3

Embodiment 3

[0026] Adjust the size of the central groove of the feed as the age of the insects increases. When the eclosion adults were found, they were transferred to a 250mL triangular flask for single-head feeding, and cotton balls soaked in distilled water were placed in the bottle, and 1- or 2-year-old fresh black pine branches were provided as food, and the branches were replaced every 2 days. About 14 days after eclosion, the male and female are paired and transferred to a 300mm×300mm×400mm stainless steel mesh cage. Take a black pine section with a length of 15 cm and a diameter of 2.5-5.5 cm, seal both ends with paraffin to prevent water loss, and place it upright on the four corners of the insect cage for the female to lay eggs. A wide-mouth bottle filled with distilled water is placed in the center of the insect cage, and fresh 1 or 2a raw healthy black pine branches are inserted inside as supplementary nutritional food. Replace branches and wood sections at 2d intervals.

[...

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Abstract

The invention discloses the forage for artificial breeding of Monochamus alternatus Hope and method for preparing the same. The forage comprises 90-110 g of pine wood chip, 18-22 g of cane sugar, 10-15 g of yeast powder, 35-45 g of agar, 1.8-2.1 g of sodium benzoate, 0.9-1.2 g of mythyl p-hydroxybenzoate, 10 mL of H2SO4 (0.5 mol/L), 15-25 g of wheat germ powder, 1.2-1.5 g of cholesterol, 3-4.5 g of ascorbic acid, 18-21 g of casein, 0.7-1.1 g of choline and 750-850 mL of water. The forage provided by the invention has the advantages of abundance in resources, convenient manufacture and low cost, and the method for preparing the same is simple and workable. The development time of larvae fed with the above forage is significantly shortened, compared with that of larvae under natural circumstance in the forest. The development period of larvae that develop from egg development to adult emergence lasts for about 100 days. The forage is applicable to the large-scale breeding of Monochamus alternatus Hope larvae. Reproduction and breeding of Monochamus alternatus Hope at controlled temperatures can be realized to ensure the supplies of experimental populations of different stages, different instars and stable activities.

Description

technical field [0001] The invention relates to artificial cultivation technology of Monochamus alternatus, in particular to a feed for artificially raising Monochamus alternatus and a preparation method thereof. Background technique [0002] Monochamus alternatus ( Monochamus alternatus ) is the main stem-boring pest that harms pine trees, and also spreads the devastating disease pine wood nematode ( Bursaphenchus xylophilus ) vector insects are listed as international quarantine objects. Its larvae and pupae live in the xylem, and adults mainly move in the upper part of the canopy, so it is difficult to collect the longhorn beetle needed for the experiment. Even if the monochamus monochamus is collected in the forest, it cannot be guaranteed due to the limitation of season, quantity, age and activity. The requirements of the experiment on the age, stage, physiological state and other conditions of the tested longhorn beetle. Therefore, it is necessary to study its indo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/16A23K1/14A23K10/30A23K20/10A23K20/147A23K20/163A23K20/20A23K50/90
Inventor 郝德君王焱樊斌琦周艳琼
Owner NANJING FORESTRY UNIV
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