Pepper anthracnose resistance living body identification method and application

A technology of pepper anthracnose and identification method, applied in the direction of application, microbe-based method, botanical equipment and method, etc., to achieve accurate and stable identification results, controllable environmental conditions, and avoid the effects of external climate conditions and soil environment

Pending Publication Date: 2022-06-10
天津市农业科学院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the isolated fruit is used as the material for identification, ignoring the systemic resistance response of the plant itself

Method used

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  • Pepper anthracnose resistance living body identification method and application
  • Pepper anthracnose resistance living body identification method and application
  • Pepper anthracnose resistance living body identification method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 3

[0026] The tested capsicum anthracnose was isolated from capsicum fruit ( C. capsici ), preserved by the vegetable ward of the Institute of Plant Protection, Tianjin Academy of Agricultural Sciences.

[0027] (1) Cultivate pepper seedlings, the same as the method in Example 1.

[0028] (2) Prepare the capsicum anthracis spore suspension for inoculation. In this experiment, capsicum anthracnose was used as black spot anthrax ( C. capsici ). Transfer the pathogenic bacteria to the PDA medium, culture in the dark at 28°C for 7 days, then brush the spores on the petri dish with sterile water, filter the mycelia with double-layer gauze, and prepare the concentration to be 6.5×10 5 spores / mL of spore suspension for inoculation.

[0029] (3) Live inoculation of capsicum anthracnose bacteria and post-inoculation management are the same as in Example 1.

[0030] (4) Disease investigation and variety resistance evaluation are the same as in Example 1.

[0031...

Embodiment 2

[0034] The tested capsicum anthracnose bacteria were 4 kinds of pathogenic bacteria isolated from pepper fruits and seeds, among which the flat-headed anthracnose bacteria ( C. trnucatum ) and black dot anthrax ( C. capsici ), preserved by the Vegetable Ward Room of the Institute of Plant Protection, Tianjin Academy of Agricultural Sciences; Gleospora anthracnose ( C. gloeosporioides ) and Anthracnose oxysporum ( C. acutatum ), preserved by the Seedling Disease Room of the Institute of Plant Protection, Tianjin Academy of Agricultural Sciences.

[0035] (1) Cultivate pepper seedlings, the same as the method in Example 1.

[0036] (2) Prepare the capsicum anthracis spore suspension for inoculation. In this test, four kinds of capsicum anthracnose bacteria were used, namely flat head anthracnose bacteria ( C. trnucatum ), black dot anthrax ( C. capsici ), Glosporum anthracnose ( C. gloeosporioides ) and Anthracnose oxysporum ( C. acutatum ). Transfer the pathogen...

Embodiment 3

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Abstract

The invention provides a pepper anthracnose resistance living body identification method and application. The method specifically comprises the steps of cultivating pepper seedlings, preparing pepper colletotrichum spore suspension, inoculating pepper colletotrichum living bodies, managing after inoculation, investigating illness conditions, evaluating variety resistance and the like. The method comprises the following steps: inoculating pepper seedlings by spraying colletotrichum spore suspension when the pepper seedlings grow to have 4-6 true leaves, investigating disease indexes of each plant according to an established pepper anthracnose seedling stage disease grading standard in the fifth to seventh days after inoculation, and evaluating the anti-infection characteristics of different pepper varieties. The method provided by the invention is a pepper anthracnose living body inoculation identification method, the operation steps are simple and efficient, the identification period is short, the result is reliable and stable, the repeatability is high, the resistance level of an identification material can be accurately reflected, and a technical support is provided for pepper anthracnose resistance resource screening and disease-resistant variety breeding and evaluation.

Description

technical field [0001] The invention belongs to the field of identification of crop disease resistance, and in particular relates to a live identification method of capsicum resistance to anthracnose and an application thereof. Background technique [0002] Pepper anthracnose is caused by the genus Anthracnose ( Colletotrichum sp.) is a global disease caused by fungi, and it is one of the main diseases that endanger pepper production. Capsicum anthracnose mainly damages the leaves and fruits of peppers. The main manifestations on the leaves are gray-brown, nearly circular lesions with concentric ring patterns. Water-soaked yellow-brown lesions are formed on the fruit at the initial stage of infection, and later, the lesions are sunken, nearly round or irregular, with small black spots on the lesions, and reddish slime overflows when wet. Pepper anthracnose pathogens are rich in species, and at least 24 pathogens have been reported to cause pepper anthracnose at home and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18A01G31/00C12R1/645
CPCC12Q1/18A01G31/00G01N2333/37
Inventor 高苇杨利娟王勇霍建飞贲海燕姚玉荣刘晓琳张春祥
Owner 天津市农业科学院
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