Resistance identification method of garlic tip blight

A resistance identification, garlic leaf technology, applied in the direction of microbial-based methods, biochemical equipment and methods, microbial measurement/inspection, etc., to achieve objective identification results, consistent responses, and accurate responses

Active Publication Date: 2019-08-13
XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the current lack of a simple, quick and reliable method for identifying resistance to garlic leaf spot, the present invention provides a method for identifying resistance to garlic leaf blight, which can be carried out indoors , easy to operate, high reliability

Method used

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  • Resistance identification method of garlic tip blight
  • Resistance identification method of garlic tip blight
  • Resistance identification method of garlic tip blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] In the present embodiment, the feasibility determination of the indoor garlic leaf blight resistance identification of the scale buds inoculated with acupuncture is carried out, and the method is as follows:

[0032] (1) Isolation and preparation of pathogenic bacteria: Collect leaves with typical symptoms of garlic leaf blight from the field, bring them back to the laboratory, isolate the pathogenic bacteria according to the tissue separation method, and check back inoculation according to Koch's law; then two weeks before inoculation indoors, in the Cultivate leaf blight fungus spores on PCA plates at 25°C; then wash the cultivated leaf blight fungus plates with sterile water to obtain spore suspension, and dilute the concentration of spore suspension to 1.0×10 6 A / mL or so;

[0033] (2) Preparation of varieties to be inoculated: separate the garlic variety "Xusuan No. 6" and peel off the skin. Select 10 scale buds with uniform size, no wounds, and no lesions, soak t...

Embodiment 2

[0037] Using the method of Example 1, three garlic varieties, "Xu Suan No. 6", "Zhongmu Garlic" and "Yulin White Garlic", were inoculated. 15 garlic cloves were inoculated for each variety, and each garlic clove was inoculated into 2 wells, which were divided into 3 replicates. After 30 days of cultivation, the garlic cloves were taken out. The lesion diameter was measured by the cross method, and the disease severity was calculated with "Xu Suan No. 6" as the control variety:

[0038] Severity = lesion diameter of the garlic to be tested ÷ lesion diameter of the control variety.

[0039] Table 1 Indoor resistance identification results and field incidence of three varieties of garlic leaf blight

[0040]

[0041] Table 1 shows the results of identification of garlic cultivar leaf blight resistance. The average lesion diameter of the variety "Xu Suan No. 6" was 4.28 mm, and the severity was 1.00; the average lesion diameter of "Zhongmou Garlic" was 5.54, and the severity...

Embodiment 3

[0044] (1) Take 200 g of fresh peeled potatoes and 200 g of fresh carrots, cut into small pieces, add 1000 mL of distilled water, and boil for 10-20 min. Filter with gauze, add distilled water to 1000 mL. Add 8-10 g of agar powder, heat to melt, subpackage, and autoclave at 121°C for 20 minutes to obtain PCA medium;

[0045] Two weeks before indoor inoculation, cultivate the spores of Leaf blight on the PCA plate at 25°C; then rinse the cultivated plate of Leaf blight with sterile water to obtain a spore suspension, and dilute the concentration of the spore suspension to 1.0×10 6 A / mL or so;

[0046] (2) Select the scale buds of 11 garlic varieties to be identified and "Xu Suan No. 6" with the same size, no wounds, and no lesions, soak them in 1% NaClO solution for 2 minutes, then rinse them with sterile water for 3 times for backup use;

[0047] (3) Inoculation of pathogenic bacteria: Dip a 30G blood collection needle into the spore suspension, and inoculate the scale buds...

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Abstract

The invention provides a resistance identification method of garlic tip blight. The resistance identification method comprises the steps that a variety with moderate resistance is adopted to serve asa check variety, and bulbils of the check variety and bulbils of varieties to be identified are punctured, inoculated with garlic tip blight bacterial spore suspension and subjected to moisturizing culture; after moisturizing culture, a cross method is adopted to measure the diameters of disease spots and calculate the disease occurrence severity, and according to the disease occurrence severity,resistance of the varieties to be identified to garlic tip blight is evaluated, wherein the varieties are evaluated to be resistant to garlic tip blight when the disease occurrence severity is equal to or smaller than 1.1, are evaluated to be moderate resistant to garlic tip blight when the disease occurrence severity is larger than 1.1 or equal to or smaller than 1.2, and are evaluated to be infected when the disease occurrence severity is larger than 1.2. By the adoption of the provided resistance identification method, the identification results are objective, operation is simple, no special techniques are needed, the occupied space is small, the method can be completed in a laboratory, and environment conditions are easy to control; the identification results are identical to field disease resistance results and can accurately reflect the resistance level of the garlic varieties to garlic tip blight.

Description

technical field [0001] The invention relates to a method for identifying the ability of garlic to resist pathogenic bacteria, in particular to a method for identifying resistance to garlic leaf blight, and belongs to the technical field of agricultural plant protection. Background technique [0002] Garlic tip blight (garlic tip blight) is a soil-borne fungal disease, and the sexual stage pathogen is Ascomycetes Pleospora , the asexual stage is Deuteromycetes sp. Stemphylium , and mainly in the asexual stage of infection. The disease is one of the common diseases in garlic production. Due to the continuous change of farming system and cultivation ecological environment, it often occurs in a large area and causes serious economic losses. The disease mainly harms the leaves or pedicels of garlic. Leaf infection usually begins at the tip of the leaf or other parts of the leaf. It initially appears as small white dots, and then expands into irregular or oval gray-white or gra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12Q1/04C12R1/645
CPCC12Q1/18C12Q1/04
Inventor 赵永强杨峰樊继德陆信娟刘灿玉张碧薇杨艳史新敏
Owner XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
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