Method for rapidly identifying tomato bacterial wilt seedling stage resistance through injection inoculation method

Abstract

The invention belongs to the technical field of agricultural breeding, and provides a method for rapidly identifying tomato bacterial wilt seedling stage resistance by an injection inoculation method, wherein the method comprises the steps of tomato material seedling preparation, ralstonia solanacearum inoculation liquid preparation, injection inoculation and disease resistance observation and identification. The injection inoculation comprises the steps: when a tomato seedling grows to have 5-6 leaves, sucking ralstonia solanacearum inoculation bacterial liquid by using an injector, aligning a needle head of an injector to the middle of a first stem of the seedling, puncturing the stem along the inclined lower direction of the stem, returning the needle head to a position close to a puncturing point in the stem, and injecting the bacterial liquid until the bacterial liquid overflows from the stem. According to the method, the bacterial wilt resistance of a breeding population material can be rapidly identified in the seedling stage, the tomato bacterial wilt resistance breeding efficiency is improved, and a simple, convenient and rapid detection method can be provided for research on tomato bacterial wilt resistance germplasm resource screening, a disease-resistant molecular mechanism and the like.

Description

technical field [0001] The invention belongs to the technical field of agricultural breeding, and in particular relates to a method for quickly identifying tomato bacterial wilt seedling stage resistance by injection inoculation. Background technique [0002] Tomato (Lycopersicon esculentum Mill.) is one of the important vegetable crops in my country. According to incomplete statistics, the planting area of ​​tomato in my country is nearly 20 million mu, and its planting area and output both rank first in the world. However, soil-borne diseases are becoming more and more serious during the long-term continuous tomato production. Tomato bacterial wilt is a worldwide devastating soil-borne disease caused by Ralstonia solanacearum, which commonly occurs in the Yangtze River Basin and south of my country. , resulting in huge economic losses and a serious threat to the healthy and sustainable development of my country's tomato industry. At present, there is no good control metho...

AI Technical Summary

Problems solved by technology

Field natural identification is to carry out field identification under the creation of natural conditions that are conducive to the disease. However, there are many factors that affect the disease in the field, such as climate conditions, soil environment, and the number of pathogenic microorganisms. Large, unstable results, poor repeatability, and there are often differences in identification results in different years

Artificial inoculation identification can overcome the above shortcomings of natural identification in the field, and the environmental conditions are relatively controllable, which greatly improves the stability and repeatability of the identification results. There are still some disadvantages in early stage resistance identification. For example, the identification time is long, it takes 2 weeks, and the seedlings need to be replanted twice after inoculation. The inoculation requires a large amount of bacterial solution, which is time-consuming and labor-intensive, and the efficiency is low.

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