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Quick tubercle myco-bacillus culture medium

A technology of Mycobacterium tuberculosis and culture medium, which is applied in the field of microorganisms, can solve the problems of long drug sensitivity cultivation time, difficult research work, and few types of drugs, and achieves the effects of short drug sensitivity cultivation time, wide application range and many types of drugs.

Inactive Publication Date: 2006-11-08
李洪敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 2. The strains cannot be stored for a long time
The culture medium of the prior art cannot make tuberculosis survive for a long time, which brings many difficulties to the research work
[0008] 3. The drug sensitivity culture takes a long time, and the types of drugs tested are few
Not only the cultivation time is long, but fresh eggs cannot be sterilized by high temperature, only pasteurized
Moreover, the culture medium is turbid, and it is difficult to observe the primary growth of Mycobacterium tuberculosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The preparation of embodiment 1 rapid mycobacterium tuberculosis culture medium (one)

[0063] formula:

[0064] Tryptone 6g Trisodium Citrate 4g

[0065] Sodium Gluconate 4.5g Ferric Ammonium Citrate 0.4g

[0066] Pyrrolidine Hydrochloride 0.1g Uracil 1.5g

[0067] 2% malachite green aqueous solution 18ml magnesium sulfate 1.0g

[0068] Glycerin 130ml Potato Flour 17g

[0069] Agar 18g Polymyxin B 1.5ml

[0070] Calf Serum 120ml Distilled Water 880ml

[0071] The preparation method of the culture medium: first mix the distilled water and glycerin, then add each component in turn, adjust the pH value to 6.6-6.8 with 10% HCl after dissolving, and sterilize under high pressure of 0.7×105pa for 20 minutes. Cool to 56°C. , add sterilized calf serum, mix evenly, divide into 18mm~150mm test tubes, each tube is 7ml, the height of the inclined plane accounts for 2 / 3 of the test tube, after 24 hours of sterility testing at 37°C, store in a refrigerator at 4°C for use. ha...

Embodiment 2

[0072] The preparation of embodiment 2 rapid mycobacterium tuberculosis culture medium (two)

[0073] Tryptone 7g Glycerin 140ml

[0074] Sodium Gluconate 5g Agar 17g

[0075] Pyrrolidine Hydrochloride 0.1g Calf Serum 130ml

[0076] 2% malachite green aqueous solution 18ml distilled water 880ml

[0077] Preparation method is as embodiment 1.

Embodiment 3

[0078] The preparation of embodiment 3 rapid Mycobacterium tuberculosis culture medium (three)

[0079] Tryptone 7g Calf Serum 130ml

[0080] Sodium Gluconate 4.5g Trisodium Citrate 4g

[0081] Pyrrolidine Hydrochloride 0.1g Ferric Ammonium Citrate 0.4g

[0082] 2% malachite green aqueous solution 18ml magnesium sulfate 0.5g

[0083] Glycerin 140ml Nalidixic Acid 1.0ml

[0084] Agar 17g Distilled water 880ml

[0085] Preparation method is as embodiment 1.

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Abstract

The present invention relates to a kind of quick tubercle mycobacillus culture medium. The quick tubercle mycobacillus culture medium has tryptone, sodium gluconate, pyritinol hydrochloride, water solution of malachite green, glycerin, agar, calf blood and other components. The quick tubercle mycobacillus culture medium has short bacterial culture time and short spawn identification time, and may be used in quick culturing and identification of tubercle mycobacillus and non-tubercle mycobacillus, the drug sensitivity test and the long term preservation of tubercle mycobacillus and non-tubercle mycobacillus. The culture medium has simple preparation process, easy observation of culture result and wide application range.

Description

Technical field: [0001] The invention relates to the field of microorganisms, in particular to a type of culture medium for rapid culture, identification, drug sensitivity test and long-term storage of tuberculosis and non-tuberculosis mycobacteria. Background technique: [0002] Currently, the tuberculosis epidemic is on the rise worldwide, and multidrug-resistant tuberculosis (MDR-TB) and nontuberculous mycobacterial disease (NTM) have increased dramatically and spread widely. The treatment of MDR-TB disease depends on the detection of drug resistance of Mycobacterium tuberculosis, early diagnosis and acquisition of drug resistance data of Mycobacterium tuberculosis, and the development of targeted comprehensive treatment plan (including new drugs), is one of the urgent tasks to be solved in the control of tuberculosis. Therefore, the use of tuberculosis culture medium is very important. [0003] There are following main deficiencies in the tuberculosis culture medium of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 李洪敏
Owner 李洪敏
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