Watermelon hybridization breeding and hybrid identification method

Abstract

The invention discloses a watermelon hybridization breeding and hybrid identification method. The method comprises the following steps that 1, watermelon leaf yellow spot inheritable characters are introduced into a male parent of a hybridized combination, and the male parent is made to express the leaf yellow spot inheritable characters; 2, the male parent with the leaf yellow spot inheritable characters is hybridized with a female parent with no leaf yellow spot inheritable characters to obtain the first generation of hybrid, and the new combination or variety of watermelon is obtained; 3, further seeding and emergence observation are further performed on seeds of the first generation of hybrid, single plants with no yellow spots on leaves are not hybridization plants, single plants with yellow spots on leaves are hybridization plants, and therefore the purity of hybridization seeds can be identified. The obtained hybridization seeds have the good yield and quality, non-hybridization seedlings can be accurately recognized and removed in a nursery stage, economic losses brought to production due to insufficient seed purity are avoided, hybridization identification can be performed, the requirement for rapid and visual watermelon hybridization identification is met, and the good market prospect is achieved.

Description

technical field [0001] The invention relates to the field of watermelon breeding, in particular to a watermelon hybrid breeding and hybrid identification method. Background technique [0002] my country is the world's largest producer of watermelon, with an area of ​​more than 30 million mu of watermelon cultivation, and the watermelon varieties used in production are all hybrids, but the identification of hybrids has always been a technical problem. The methods for identifying watermelon hybrids in the prior art mainly include general planting identification methods and molecular marker identification methods. [0003] Among them, the general planting identification method is to take the representative samples of the hybrids harvested in the current year and sow them in the field before planting in the second year, and identify the hybrids by identifying whether the performance traits of the sown watermelon (mainly the fruit traits) conform to the variety traits. Since a w...

AI Technical Summary

Problems solved by technology

However, the general planting identification method still has the following disadvantages: limited by natural environmental conditions, it cannot be identified early, it occupies a large area, takes a long time, and the cost is high, and it cannot supervise and deal with the quality disputes after the sale of seeds in a timely manner. Bring a lot of inconvenience to watermelon seed production, sales and melon farmers

However, the molecular marker identification method still has the following disadvantages: the identification is not intuitive, and the operation requirements are strict, it is easily affected by the test conditions, the requirements for technicians are high, the identification cost is high, and the applicability is poor.

The shortcoming that this technical method exists is: the back green seedling is a minority, and the normal green seedling is the majority, it is not easy to distinguish the hybrid seedlings obtained by other normal green seedlings other than the male parent that is easy to appear or mixed.

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