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True or false and new or old paddy rice seed identifying method

A technology for rice seeds and authenticity identification, applied in the field of rice seeds, can solve the problems of unfavorable rare seed identification, poor measurement accuracy, long measurement time and the like, and achieve the effects of short identification time, high accuracy and easy operation.

Inactive Publication Date: 2009-12-23
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is following shortcoming in these two kinds of methods: the one, the time is long, for example needs a week with the new and old degree of measuring seed with hyperosmotic germination method, and isozymography analysis method needs to reach more than one week sometimes; The 2nd, seed consumption is bigger, A single index measurement is not conducive to the identification of rare seeds
However, the measurement time for identifying rice seeds is relatively long, and the required time is about 1 hour; and the measurement is the weak light emitted by rice seeds after absorbing water, so the measurement accuracy is poor and the sensitivity is also low.

Method used

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  • True or false and new or old paddy rice seed identifying method
  • True or false and new or old paddy rice seed identifying method
  • True or false and new or old paddy rice seed identifying method

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Identification of authenticity of No. 1 and No. 2 rice seeds

[0029] Firstly, select dry rice seeds with the same size and intact seed coat to be detected, and store them at a relative humidity of 50% before measurement. When measuring, the rice seeds are shelled and ground into powder, put into a 2 ml quartz cuvette, placed in a dark box, and then add 1 ml of MCLA solution, so that the seed powder is completely submerged in water, and then use The self-made single photon counter measures its chemiluminescence intensity for 20 minutes, the measurement temperature is 30 (± 2) ℃, and the relative humidity is 50 (± 5)%; figure 1 The shown chemiluminescence intensity change curve 1 (corresponding to No. 1 seed) and curve 2 (corresponding to No. 2 seed).

[0030] Will figure 1 Compared with the known curves, it was identified that curve 1 represents Fengsiachan seeds, and curve 2 represents Fengxiuzhan seeds.

Embodiment 2

[0032] Identification of No. 3, No. 4, and No. 5 Fengsi accounted for the storage time of rice seeds (that is, the degree of newness and oldness)

[0033] Firstly, select dry rice seeds with the same size and intact seed coat to be detected, and store them at a relative humidity of 45% before measurement. When measuring, the rice seeds are shelled and ground into powder, put into a 2 ml quartz cuvette, placed in a dark box, and then add 1 ml of MCLA solution, so that the seed powder is completely submerged in water, and then use The self-made single photon counter measures its chemiluminescence for 20 minutes, the measurement temperature is 25 (± 2) ℃, and the relative humidity is 45 (± 5)%; figure 2 The shown chemiluminescence intensity change curve 3 (corresponding to No. 3 seed), curve 4 (corresponding to No. 4 seed), and curve 5 (corresponding to No. 5 seed).

[0034] Get the average value of the chemiluminescence intensity 5 minutes after the start of measurement, then ...

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Abstract

The disclosed identification method for rice seed bases on that the seed chemical light strength curve feature and average strength during early expand period. This invention is fast and low cost.

Description

technical field [0001] The invention relates to the field of rice seeds, in particular to a method for identifying rice seeds. Background technique [0002] At present, the authenticity, newness and oldness of rice seeds are judged mainly by measuring the germination rate, such as the hyperosmotic germination method, or using the isozyme analysis method. The principle of the hyperosmotic germination method is that the germination ability of seeds in a hyperosmotic solution is significantly positively correlated with their vigor, based on which the old and new rice seeds can be identified by testing the vigor of the seeds. The principle of the isozyme analysis method is that isozymes are the products of gene expression, which directly reflect the genetic differences between organisms and the genetic variation in the phylogenetic process. Based on this, the isozyme electrophoresis analysis technology is used to identify the authenticity of seeds. But there is following shortc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N33/00G01N1/28
Inventor 邢达黎坚
Owner SOUTH CHINA NORMAL UNIVERSITY
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