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A rapid judgment method of "fried charcoal storage" prepared from orientalis leaves

A technology of frying charcoal storage and arborvitae leaves, which is applied in the field of rapid judgment of "fried charcoal storage" prepared by arborvitae leaves, can solve the problems of complicated operation, long detection time, small application range, etc., and achieves clear color development, Easy to use effect

Active Publication Date: 2019-10-25
SHANDONG ACAD OF CHINESE MEDICINE
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Problems solved by technology

Because charcoal medicines sold in the market are generally under-fried or over-fried, in order to improve the identification accuracy of "fried charcoal storage", modern methods mainly study the pharmacology and chemical components of charcoal medicines, and formulate the identification method. Quantitative basis for "fried charcoal storage", but these methods are complicated to operate, take a long time to detect, and need to use precision instruments such as microscopes, chromatographs, spectrometers, etc., and the scope of application is small

Method used

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  • A rapid judgment method of "fried charcoal storage" prepared from orientalis leaves
  • A rapid judgment method of "fried charcoal storage" prepared from orientalis leaves
  • A rapid judgment method of "fried charcoal storage" prepared from orientalis leaves

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Embodiment 1

[0033] A method for quickly judging the "fried charcoal storage property" prepared from cypress leaves, the steps comprising:

[0034] 1) Take 1g of the powder of the sample to be tested (fried with moderate charcoal), add 20mL of 70% (W / V) methanol aqueous solution to dissolve, sonicate for 30min, filter, take the filtrate, evaporate it to dryness in a water bath, add 2ml of methanol to dissolve, and obtain the test solution;

[0035] 2) Take quercetin standard substance, add ethanol to prepare a 0.1mg / ml solution to obtain quercetin reference solution; take quercetin standard substance, add ethanol to prepare a 0.1mg / ml solution to obtain quercetin Reference substance solution;

[0036] 3) Thin-layer chromatography (Pharmacopoeia IV General Rules 0502): Take 3 μL each of the test solution, quercetin reference solution, and quercetin reference solution, respectively, and spot on the same silica gel G thin-layer plate, with ethyl acetate -Methanol-water (100:17:13) is the dev...

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Abstract

The invention relates to a rapid judgment method for 'carbonizing' of processing of cacumen biotae. A method for simultaneously identifying quercitrin and quercetin by TLC is used as a measuring basisof 'carbonizing' of the cacumen biotae, a method of secondary extending on the same thin layer is adopted, two ingredients including the quercitrin and the quercetin are identified simultaneously, and thus, it shows that requirements of 'carbonizing' of the cacumen biotae are met. By the method, the two ingredients including the quercitrin and the quercetin which show 'carbonizing' of the cacumenbiotae can be simultaneously identified on the same thin layer plate, and thus, judgment basis of 'carbonizing' of the cacumen biotae can be obtained. The identifying time is short, the method is accurate, stable and feasible, effective separation of the quercitrin from the quercetin in cacumen platycladi carbonisatum can be realized, and after separation, spots are clear.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and relates to a method for judging the processing of traditional Chinese medicines, in particular to a method for quickly judging the "fried charcoal storage properties" of the processing of oriental cypress leaves. Background technique [0002] Arborvitae arborvitae Platycladus orientalis (L.) Branches and leaves of Franco. The raw product of oriental cypress leaves is effective in clearing heat and cooling blood, relieving cough and eliminating phlegm, and is used for various hemorrhagic diseases caused by excessive blood heat, cough with excessive phlegm, damp tropical climate and hair loss. After frying charcoal, the cold and cool nature tends to be peaceful, and it is specially used to reduce astringency and stop bleeding. [0003] At present, the "fried charcoal storage" of charcoal medicine is mostly distinguished according to its properties, color, and smell, whi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90G01N30/94
CPCG01N30/90G01N30/94
Inventor 石典花张军戴衍朋孙立立
Owner SHANDONG ACAD OF CHINESE MEDICINE
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