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Method for poducing transgene seeds of hybrid paddy rice in multiple resistances

A production method and transgenic technology, which can be applied in the fields of biochemical equipment and methods, genetic engineering, plant genetic improvement, etc., can solve the problems of inability to produce in large areas and the purity of hybrid rice seeds is not large enough, and achieve resistance to borers and rice planthoppers. Harm, high identification efficiency, and the effect of improving purity

Inactive Publication Date: 2005-06-15
天津市水稻研究所 +2
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to address the above-mentioned problems in the prior art, and provide a production method for multi-resistant transgenic hybrid rice seeds, which can not only overcome the problem that hybrid rice seeds cannot be produced in a large area due to insufficient purity, but also enhance hybridization. Insect resistance in rice to increase yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0052] Example of hybrid rice seed production by three-line method

[0053] 1. Parent

[0054] 1.1 Restoration Department

[0055] 1.1.1 Transgenic receptors

[0056] Chaoyou 1 (also known as Jindao 1229), a high-quality japonica restorer line bred for Tianjin Rice Research Institute. The full growth period of cultivation in Beijing and Tianjin is 145 days, and the yield per mu is 500-600Kg. The plant height is about 110cm, the ear is big, the ear length is 22cm, and each ear has an average of 116 grains; the grain is large, the thousand-grain weight is 26-27g, and the seed setting rate is 91%. After being inspected by the Grain Quality Supervision, Inspection and Testing Center of the Ministry of Agriculture, all indicators have reached the national first-class high-quality rice standard.

[0057] 1.1.2 Transgenic restorer lines

[0058] The whole growth period is the same as that of the transformed recipient, about 145 days. The plant height is about 115cm, the ear len...

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Abstract

A process for preparing the seeds of multi-resistance transgenic rice includes transferring the anti-herbicide gene and the anti-borer gene to the restoring line of three-line or two-line crossed rice, transferring the plant hopper-resistant gene to the maintenance line of three-line crossed rice, hybridizing between sterile line and maintenance line, and hybridizing between transgenic restoring line and transgenic sterile line.

Description

technical field [0001] The invention relates to a production method of hybrid rice seeds, in particular to a production method of multi-resistance transgenic hybrid rice seeds. Background technique [0002] Rice is the staple food for nearly half of the world's population. About one-third of the world is killed by pests and weeds every year. It is estimated that the economic losses caused are more than 12 million US dollars per year. In order to deal with pests and weeds, about 2 million tons of pesticides are produced every year. For example: Chilo borer and rice planthopper are two major insect pests in northern paddy fields, which can reduce rice yield by 10%-20% every year, and severe cases may even cause crop failure. At present, the main measures to prevent and control insect pests are to use a large number of insecticides such as dimehypo and carbofuran. The annual consumption can reach more than 10 million kilograms, and the value is about 20 million yuan. This no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/00A01H1/02C12N15/29
Inventor 李艳萍牛芝霞牛景邹美智孙海波周维朱祯邓朝阳徐鸿林
Owner 天津市水稻研究所
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