Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

The application of its sequence as a dna barcode in the identification of Jiaxing Plum and its identification method

A barcode and sequence technology, applied in the field of molecular identification, can solve different and difficult problems, and achieve the effects of high repeatability, short identification time and high detection accuracy

Active Publication Date: 2019-02-05
ZHEJIANG JIAXING AGRI SCI ACADEMY INST
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the fruit appearance of Prunus chinensis is affected by different cultivation and management methods, soil and climate conditions, the color of the fruit is often different. It is difficult to distinguish Plum prunes from other varieties of Plums only by fruit shape. Relying on traditional morphological taxonomy methods in Jiaxing There are limitations in the identification of Li

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • The application of its sequence as a dna barcode in the identification of Jiaxing Plum and its identification method
  • The application of its sequence as a dna barcode in the identification of Jiaxing Plum and its identification method
  • The application of its sequence as a dna barcode in the identification of Jiaxing Plum and its identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Molecular identification of Plum plum

[0033] The samples were collected from the Plum Horticultural Science Research Institute of Jiaxing City, Zhejiang Province, and the 19 identified samples were Zhejiang Jiaxing Plum (early maturity) (T1), Zhejiang Jiaxing Plum (late maturity) (T2), Ningbo Eggplant Plum (T3), Zhoushan Jintang Plum (T4), Fujian Red Heart Plum (T5), Hubei Yuhuang Plum (T6), Guizhou Jiuqian Plum (T7), Anhui Amaranth Plum (T8), Jiangxi Cinnabar Plum (T9), Shandong Beef Heart Plum ( T10), American Dinosaur Egg (T11), American La Roda (T12), American Rose Queen (T13), American Angorino (T14), Japanese Dashi Zao (T15), Japanese White Plum (T16), Japanese Autumn Ji (T17), Guiling of Japan (T18), Miss Lee of New Zealand (T19). Please refer to the attached photo for the photo of the plum fruit figure 1 .

[0034] Genomic DNA of the above-mentioned leaves of Plum plum was extracted by CTAB method, and PCR amplification was performed using the fol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of ITS (internal transcribed spacer) sequence as a DNA barcode in identifying Jiaxing Zuili (Prunus salicina lindl.) and an identifying method and belongs to the technical field of molecular identification. The identifying method comprises: amplifying ITS sequence of a sample to be tested by using PCR (polymerase chain reaction) and carrying out sequencing; results show that if both basic group 165bp and basic group 195bp of the ITS sequence are 'T', it is determined that the sample to be tested is Jiaxing Zuili. The invention also discloses a method for identifying early maturing and late maturing varieties of Jiaxing Zuili; if the basic group sequence of the ITS sequence is shown as in SEQ ID No. 1, it is determined that the sample to be tested is an early maturing variety of Jiaxing Zuili; if the basic group sequence of the ITS sequence is shown as in SEQ ID No. 2, it is determined that the sample to be tested is a late maturing variety of Jiaxing Zuili. The ITS sequence is used herein as the DNA barcode to identify Jiaxing Zuili, detection accuracy is high, detection repeatability is high, and identifying time is short.

Description

technical field [0001] The invention relates to the technical field of molecular identification, in particular to the application and identification method of the ITS sequence as a DNA barcode in the identification of Jiaxing Plum. Background technique [0002] Plum (Prunus salicina Lindl.) is a plant of the genus Prunus L. in the family Rosaceae. It is a special product in Jiaxing City, Zhejiang Province, and is also a traditional famous fruit in Zhejiang Province. The quality of the plum is delicate and fresh. When ripe, it can turn into a sweet and slightly wine-scented syrup. It has an excellent flavor and quality. It is one of the high-quality fruits that consumers love in summer. At present, in addition to the cultivation of Plum plum in Tongxiang, Haining, Haiyan, Huzhou and other places in Jiaxing, Zhejiang Province, there are also a small amount of introductions in Jiangsu Province and Anhui Province. [0003] Most of the plums are propagated by grafting, and graft...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 李军李白李斌
Owner ZHEJIANG JIAXING AGRI SCI ACADEMY INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com