Application of ITS (internal transcribed spacer) sequence as DNA barcode in identifying Jiaxing Zuili (Prunus salicina lindl.) and identifying method

Abstract

The invention discloses application of ITS (internal transcribed spacer) sequence as a DNA barcode in identifying Jiaxing Zuili (Prunus salicina lindl.) and an identifying method and belongs to the technical field of molecular identification. The identifying method comprises: amplifying ITS sequence of a sample to be tested by using PCR (polymerase chain reaction) and carrying out sequencing; results show that if both basic group 165bp and basic group 195bp of the ITS sequence are 'T', it is determined that the sample to be tested is Jiaxing Zuili. The invention also discloses a method for identifying early maturing and late maturing varieties of Jiaxing Zuili; if the basic group sequence of the ITS sequence is shown as in SEQ ID No. 1, it is determined that the sample to be tested is an early maturing variety of Jiaxing Zuili; if the basic group sequence of the ITS sequence is shown as in SEQ ID No. 2, it is determined that the sample to be tested is a late maturing variety of Jiaxing Zuili. The ITS sequence is used herein as the DNA barcode to identify Jiaxing Zuili, detection accuracy is high, detection repeatability is high, and identifying time is short.

Description

technical field [0001] The invention relates to the technical field of molecular identification, in particular to the application and identification method of the ITS sequence as a DNA barcode in the identification of Jiaxing Plum. Background technique [0002] Plum (Prunus salicina Lindl.) is a plant of the genus Prunus (Prunus L.) in the family Rosaceae. It is a specialty product in Jiaxing City, Zhejiang Province, and is also a traditional famous fruit in Zhejiang Province. The quality of the plum is delicate and fresh. When ripe, it can turn into a sweet and slightly wine-scented syrup. It has an excellent flavor and quality. It is one of the high-quality fruits that consumers love in summer. At present, in addition to the cultivation of Plum plum in Tongxiang, Haining, Haiyan, Huzhou and other places in Jiaxing, Zhejiang Province, there are also a small amount of introductions in Jiangsu Province and Anhui Province. [0003] Most of the plums are propagated by grafting...

AI Technical Summary

Problems solved by technology

Because the fruit appearance of Prunus chinensis is affected by different cultivation and management methods, soil and climate conditions, the color of the fruit is often different. It is difficult to distinguish Plum prunes from other varieties of Plums only by fruit shape. Relying on traditional morphological taxonomy methods in Jiaxing There are limitations in the identification of Li

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