Application of ITS (internal transcribed spacer) sequence as DNA barcode in identifying Jiaxing Zuili (Prunus salicina lindl.) and identifying method

A barcode and sequence technology, applied in the field of molecular identification, can solve different and difficult problems, and achieve the effects of high repeatability, short identification time, and high detection accuracy.

CN105671185AActive Publication Date: 2016-06-15ZHEJIANG JIAXING AGRI SCI ACADEMY INST
1 Cites 6 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2016-06-15

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses application of ITS (internal transcribed spacer) sequence as a DNA barcode in identifying Jiaxing Zuili (Prunus salicina lindl.) and an identifying method and belongs to the technical field of molecular identification. The identifying method comprises: amplifying ITS sequence of a sample to be tested by using PCR (polymerase chain reaction) and carrying out sequencing; results show that if both basic group 165bp and basic group 195bp of the ITS sequence are 'T', it is determined that the sample to be tested is Jiaxing Zuili. The invention also discloses a method for identifying early maturing and late maturing varieties of Jiaxing Zuili; if the basic group sequence of the ITS sequence is shown as in SEQ ID No. 1, it is determined that the sample to be tested is an early maturing variety of Jiaxing Zuili; if the basic group sequence of the ITS sequence is shown as in SEQ ID No. 2, it is determined that the sample to be tested is a late maturing variety of Jiaxing Zuili. The ITS sequence is used herein as the DNA barcode to identify Jiaxing Zuili, detection accuracy is high, detection repeatability is high, and identifying time is short.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of molecular identification, in particular to the application and identification method of the ITS sequence as a DNA barcode in the identification of Jiaxing Plum. Background technique

[0002] Plum (Prunus salicina Lindl.) is a plant of the genus Prunus (Prunus L.) in the family Rosaceae. It is a specialty product in Jiaxing City, Zhejiang Province, and is also a traditional famous fruit in Zhejiang Province. The quality of the plum is delicate and fresh. When ripe, it can turn into a sweet and slightly wine-scented syrup. It has an excellent flavor and quality. It is one of the high-quality fruits that consumers love in summer. At present, in addition to the cultivation of Plum plum in Tongxiang, Haining, Haiyan, Huzhou and other places in Jiaxing, Zhejiang Province, there are also a small amount of introductions in Jiangsu Province and Anhui Province.

[0003] Most of the plums are propagated by grafting...

Examples

Embodiment 1

[0032] The molecular identification of embodiment 1 Plum

[0033] The samples were collected from the Plum Horticultural Science Research Institute of Jiaxing City, Zhejiang Province, and the 19 identified samples were Zhejiang Jiaxing Plum (early maturity) (T1), Zhejiang Jiaxing Plum (late maturity) (T2), Ningbo Eggplant Plum (T3), Zhoushan Jintang Plum (T4), Fujian Red Heart Plum (T5), Hubei Yuhuang Plum (T6), Guizhou Jiuqian Plum (T7), Anhui Amaranth Plum (T8), Jiangxi Cinnabar Plum (T9), Shandong Beef Heart Plum ( T10), American Dinosaur Egg (T11), American La Roda (T12), American Rose Queen (T13), American Angorino (T14), Japanese Dashi Zao (T15), Japanese White Plum (T16), Japanese Autumn Ji (T17), Guiling of Japan (T18), Miss Lee of New Zealand (T19). Please refer to the attached photo for the photo of the plum fruit figure 1 .

[0034] Genomic DNA of the above-mentioned leaves of Plum plum was extracted by CTAB method, and PCR amplification was performed using the f...