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Method for rapid identification of sweet potato stem rot resistance

A technology for stem rot and sweet potato, applied in the field of screening germplasm resources and breeding materials resistant to stem rot of sweet potato, can solve the problems of difficulty in ensuring consistent inoculation amount, unstable symptoms of pathogenic sweet potato, etc., and achieve identification results The effects of stability, short identification time, and short symptom display time

Inactive Publication Date: 2014-02-05
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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Problems solved by technology

The inventor tried to use the needle-punching method and potato slice inoculation method used by American scholars in the pathogenicity identification of sweet potato stem rot, as well as several commonly used bacterial disease inoculation methods such as leaf-cutting method and spraying method, and found that these inoculation methods The symptoms of the obtained diseased sweet potato are unstable, and the inoculation amount is difficult to ensure consistency, so it is not suitable for large-scale inoculation resistance identification of sweet potato stem rot

Method used

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  • Method for rapid identification of sweet potato stem rot resistance
  • Method for rapid identification of sweet potato stem rot resistance

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Embodiment Construction

[0024] Experimental materials: 98 sweet potato germplasm resources preserved in the "National Guangzhou Sweet Potato Resource Nursery".

[0025] Experimental method: 98 sweet potato germplasm resources (see Table 1 for details) preserved in the "National Guangzhou Sweet Potato Resource Nursery" were identified for resistance to sweet potato stalk rot. According to the incidence of sweet potato, these sweet potato germplasm resources were divided into high Disease-susceptible varieties, susceptible varieties, and disease-resistant varieties.

[0026] Experimental steps: (1) Treatment and pre-cultivation of stems and branches: Take the main stems or side branches of sweet potato with normal growth, neat and disease-free from the leaf nodes of sweet potato, and trim the main stem or side branches of sweet potato with a sterile scalpel. ~ 4 stems and branches with spread leaves, the length of the stems and branches is 12-15cm, after cutting, wash the juice from the cutting mouth with w...

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Abstract

The invention relates to a method for rapid identification of sweet potato stem rot resistance. The method comprises the operation steps of: trimming a sweet potato stem or side branch into a stem branch with 3-4 extended leaves, and conducting pre-culture for 2-3 days under room temperature; when fibrous roots grow out of the stem branch, placing the stem branch horizontally, stabbing a small hole by a pipettor tip between two sections in the stem branch center without penetrating the stem branch, then placing the stem branch horizontally in a sealed plastic box paved with filter paper steeped by sterile water, and making the stem branch side stabbed with the small hole upward, absorbing 15-25 microliters of a 1*10<8>cfu / mL stem rot pathogenic bacterial suspension, and injecting the suspension into the small hole for inoculated culture; and after 20-24h of culture, observing the disease condition and conducting disease grade identification on the sweet potato stem branch. The method provided by the invention has the advantages of simple and fast operation, strong practicability, strong operability, and high identification result repeatability and reliability, and has potential social and economic significance for screening excellent germplasm resources resisting sweet potato stem rot.

Description

Technical field [0001] The invention relates to a method for quickly identifying the disease resistance of sweet potato stalk rot, in particular to a method for screening sweet potato stalk rot resistant germplasm resources and breeding materials, and belongs to the technical field of plant protection. Background technique [0002] Sweet potato (Ipomoea batatas (L.) Lam) is one of the world's food, energy and industrial raw material crops. my country is the world's largest sweet potato producer. Its planting area ranks fourth after rice, wheat and corn. In recent years, with the strong market demand for sweet potato, farmers' enthusiasm for planting and introducing new varieties has increased. Seedlings or seed potatoes flow frequently between countries and regions, coupled with disorderly planting, followed by some new developments in the main sweet potato production areas in my country. Diseases or traditional diseases have re-emerged. [0003] Sweet potato stalk rot was first di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/645
Inventor 黄立飞房伯平陈景益张雄坚李育军王章英罗忠霞黄实辉陈新亮
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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