Method for rapid identification of sweet potato stem rot resistance

Abstract

The invention relates to a method for rapid identification of sweet potato stem rot resistance. The method comprises the operation steps of: trimming a sweet potato stem or side branch into a stem branch with 3-4 extended leaves, and conducting pre-culture for 2-3 days under room temperature; when fibrous roots grow out of the stem branch, placing the stem branch horizontally, stabbing a small hole by a pipettor tip between two sections in the stem branch center without penetrating the stem branch, then placing the stem branch horizontally in a sealed plastic box paved with filter paper steeped by sterile water, and making the stem branch side stabbed with the small hole upward, absorbing 15-25 microliters of a 1*10<8>cfu / mL stem rot pathogenic bacterial suspension, and injecting the suspension into the small hole for inoculated culture; and after 20-24h of culture, observing the disease condition and conducting disease grade identification on the sweet potato stem branch. The method provided by the invention has the advantages of simple and fast operation, strong practicability, strong operability, and high identification result repeatability and reliability, and has potential social and economic significance for screening excellent germplasm resources resisting sweet potato stem rot.

Description

Technical field [0001] The invention relates to a method for quickly identifying the disease resistance of sweet potato stalk rot, in particular to a method for screening sweet potato stalk rot resistant germplasm resources and breeding materials, and belongs to the technical field of plant protection. Background technique [0002] Sweet potato (Ipomoea batatas (L.) Lam) is one of the world's food, energy and industrial raw material crops. my country is the world's largest sweet potato producer. Its planting area ranks fourth after rice, wheat and corn. In recent years, with the strong market demand for sweet potato, farmers' enthusiasm for planting and introducing new varieties has increased. Seedlings or seed potatoes flow frequently between countries and regions, coupled with disorderly planting, followed by some new developments in the main sweet potato production areas in my country. Diseases or traditional diseases have re-emerged. [0003] Sweet potato stalk rot was first di...

AI Technical Summary

Problems solved by technology

The inventor tried to use the needle-punching method and potato slice inoculation method used by American scholars in the pathogenicity identification of sweet potato stem rot, as well as several commonly used bacterial disease inoculation methods such as leaf-cutting method and spraying method, and found that these inoculation methods The symptoms of the obtained diseased sweet potato are unstable, and the inoculation amount is difficult to ensure consistency, so it is not suitable for large-scale inoculation resistance identification of sweet potato stem rot

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