10 results about "Linked protein" patented technology

The invention provides a double-layer collagen scaffold repair membrane as well as a preparation method and application thereof, and the preparation method comprises the following steps: extracting collagen from animal fur, and emulsifying to obtain a collagen emulsion; adding a cross-linking agent into the collagen emulsion, and emulsifying to obtain a cross-linked protein emulsion; pouring the cross-linked protein emulsion into a mold, and freeze-drying to obtain a cross-linked protein layer; and pouring the collagen emulsion on the cross-linked protein layer, and freeze-drying to obtain the double-layer collagen scaffold repair membrane. The repairing membrane is excellent in structure, the cross-linked protein layer provides mechanical strength and supporting performance, the collagen layer can be rapidly dissolved and attached to the contact surface, and oxygen exchange and metabolite discharge of the wound surface are facilitated; the collagen is high in purity, low in antigenicity and high in safety, any toxic and harmful chemical reagents and preservatives are not added, allergy or inflammatory reaction is avoided, and the collagen has good application prospects in the fields of medical treatment, medical beauty, beauty, skin care and the like.

The invention discloses a sequential enzymatic-ionic synergistic soybean protein isolate modification method, which comprises the following steps: dispersing soybean protein isolate in water to prepare a protein solution, adding glutamine transaminase, carrying out heating reaction to inactivate enzyme to obtain an enzyme cross-linked protein solution, adding MgCl2 into the enzyme cross-linked protein solution, and carrying out heating reaction to induce protein aggregation to obtain a modified soybean protein isolate solution; and cooling to room temperature after the reaction is finished. The invention also discloses an application of the soybean protein product obtained by the sequential enzymatic-ionic synergistic soybean protein isolate modification method as a food additive. According to the method, the sensitization of the soybean protein can be remarkably reduced, meanwhile, the unique stomach resistance-intestinal efficient release dynamic characteristic is shown, and the small intestine peptide fragment release amount is promoted to be increased.

This invention belongs to the food field, specifically relating to a hypoallergenic, gluten-free, non-wheat tiger nut fermented noodle product and its preparation method. The hypoallergenic, gluten-free, non-wheat tiger nut fermented noodle product comprises the following components: raw material powder, alkaline protease, transglutaminase, additives, and water; the raw material powder is a mixture of tiger nut meal powder and rice flour. The technical solution of this application utilizes a fermentation system using alkaline protease and transglutaminase. Alkaline protease hydrolyzes the amide and peptide bonds of proteins, causing acylation reactions that cross-link protein molecules, producing a network structure and improved flavor and quality. Transglutaminase catalyzes the acylation reaction between the amide group in glutamine and the ε-amino group on lysine peptides, causing cross-linking of protein molecules to form the target protein and produce the desired product.

Compounds, proteins, crosslinked proteins, compositions thereof, and methods of making and uses thereof. A compound, which may be an alpha-amino acid, comprises one or more beta-lactam group(s), one or more triazole groups, substituted analogs thereof, or any combination thereof. A protein comprises one or more amino acid residue(s), each residue comprising a beta-lactam group, a triazole group, or a substituted analog thereof. A protein can be made by a recombinant method using one or more compound(s). A cross-linked protein comprises one or more intramolecular crosslink(s) and / or one or more intermolecular crosslink(s). In various examples, a crosslink is formed, e.g., in solution or in vivo, by a proximity enabled beta-lactam ring opening reaction or an acyl transfer reaction between a beta-lactam group or a triazole group and a nucleophilic side-chain group, where both groups are on a single polypeptide or on different polypeptide chains. Crosslinked protein(s) can be used in methods of treatment.

The invention relates to the technical field of food preservation, and provides a tapioca pearl preservative as well as a preparation method and application thereof, and the tapioca pearl preservative comprises casein, chymosin, food-grade calcium salt, a bacteriostatic agent and water. The preparation method of the tapioca pearl preservative comprises the following steps: (1) adding casein, chymosin and food-grade calcium salt into water, heating and stirring to obtain a solution A; and (2) adding a bacteriostatic agent into the solution A, stirring and dissolving to form the tapioca pearl preservative. When in use, the tapioca pearls preservative is sprayed on the surfaces of tapioca pearls and then dried, a layer of enzymatic cross-linked protein film activated by calcium ions can be formed on the surfaces of tapioca pearls, and the film has double functions of physical barrier and chemical bacteriostasis, so that long-acting preservation is realized, and the tapioca pearls preservative has the advantages of high safety, long preservation period, low cost and the like. And the fresh-keeping method is suitable for fresh-keeping treatment of industrial production of tapioca pearls.

The invention belongs to the technical field of biomedical materials, and particularly relates to a multi-crosslinked protein preparation method, a product and application. The preparation method comprises the following steps: dissolving a collagen sample in a buffer solution, adding an antioxidant, and carrying out ultrasonic treatment to obtain a collagen solution; then adding dithio bismaleimide into the collagen solution, and stirring to form a first cross-linked protein; adding a cross-linking agent mixture into the primary cross-linked protein to form secondary cross-linked protein; and finally, adding ethylene glycol dimethacrylate and a photoinitiator into the double-crosslinked protein, uniformly stirring, and dialyzing to obtain the photoinitiator. The multi-crosslinked protein prepared by the invention has better crosslinking degree, cell survival rate and in-vitro degradation half-life period, the adopted crosslinking agent is safe and non-toxic, and the multi-crosslinked protein is suitable for skin fillers, tissue engineering scaffolds, wound dressings and other scenes with high requirements on biocompatibility.

The application provides a cytoskeleton modeling method based on dissipative particle dynamics simulation, comprising the following steps: step 1, setting a cell region in a dissipative particle dynamics simulation software; step 2, randomly distributing microfilament single chains which do not intersect each other in the cell region; step 3, randomly generating a plurality of free cross-linking protein particles in the cell region; step 4, the microfilament single chains and the cross-linking protein particles are polymerized / dissociated until the number of the cross-linking protein particles in three states tends to be stable, the three states of the cross-linking protein particles are free, combined with only one microfilament single chain and combined with two microfilament single chains; step 5, deleting the cross-linking protein particles and directly connecting two microfilament single chains connected by the same cross-linking protein particle; and step 6, calculating cytoskeleton parameters and outputting a cytoskeleton model. The cytoskeleton constructed by the application has high fidelity and presents mechanical characteristics such as viscoelasticity under the action of an external force.

This application relates to the field of bioactive peptide preparation technology, and discloses a method for preparing a blood pressure-lowering protein active peptide composition. The raw materials for this composition include a protein substrate, deionized water, alkaline protease, a complex solubilizing buffer, and a cross-linked aqueous solution containing sodium hexametaphosphate. The preparation method includes: isothermal enzymatic hydrolysis of the protein substrate; adding the complex solubilizing buffer to the hydrolysate and instantaneously heating it to construct a steric hindrance protective layer for the short peptides; subsequently injecting the cross-linked aqueous solution for high-shear cross-linking, allowing large molecular impurities to target and bind; and then directly spraying it into a vacuum flash evaporator for rapid cooling, causing the cross-linked protein network to shrink and densify; finally, centrifugation, ultrafiltration, concentration, and drying are performed to obtain the finished product. This invention prevents the active short peptides from co-precipitating with impurities under heat, effectively maintaining the blood pressure-lowering activity of the peptides, and densifying the impurity flocs, thereby improving the separation and sludge removal efficiency and the anti-fouling ability of the ultrafiltration membrane.

Methods for making hemoglobin based blood substitute preparations and hemoglobin based blood substitute preparations. The methods involve preparing a low purity erythrocyte protein fraction comprising hemoglobin protein and endogenous non-hemoglobin protein complement, and chemically modifying the proteins in the protein fraction to form a cross-linked hemoglobin containing blood substitute preparation. The low purity erythrocyte protein preparation can contain from at least about 0.2% (mole / mole) up to about 20% (mole / mole) endogenous non-hemoglobin protein complement. At least about 90% (mole / mole) of the hemoglobin proteins can be cross-linked, so that the average molecular mass of cross-linked proteins comprising hemoglobin protein molecules in the preparation is at least about 300 kDa. The preparations can be used to prepare finished blood substitute formulations for in-vivo and ex-vivo use.

The invention discloses polypeptide type micromolecule glue and application thereof in preparation of cosmetics with a skin anti-aging effect. The polypeptide type small molecule glue comprises a hyaluronic acid binding peptide fragment and a proteoglycan binding peptide fragment. The polypeptide type small molecule glue provided by the invention is polypeptide with biological activity, the polypeptide can be combined with hyaluronic acid and proteoglycan after entering cells, the function of connecting the hyaluronic acid and the proteoglycan to protein 1 (HAPLN1) can be replaced, the content of collagen which is gradually reduced along with skin aging can be recovered to a certain extent, and the collagen type small molecule glue has the advantages of being good in biocompatibility, good in stability and good in stability. The composition may become a promising innovative strategy for preventing or repairing the aged skin.