31 results about "Collagen molecule" patented technology

The invention belongs to the technical field of biomedical materials and regenerative medicine, and relates to collagen-based injectable fiber hydrogel as well as a preparation method and application thereof. According to the injectable fiber hydrogel, methacrylated collagen and dithiothreitol are subjected to a thiol-ene click reaction rapidly under the action of a photoinitiator through illumination, a primary cross-linked network is formed, and the injection and in-situ gel forming requirements are met; then under the conditions that the pH is 6.5-8.0 and the temperature is 30-39 DEG C, collagen molecules are self-assembled into a nanofiber structure, and a bionic network similar to a natural extracellular matrix is obtained. The hydrogel disclosed by the invention has the advantages of rapid gelation, structural biomimetic property and biological activity; residual sulfydryl endows the hydrogel with active oxygen scavenging capacity, so that oxidative stress can be relieved, and immune response can be regulated; the fibrotic structure promotes cell adhesion, migration and tissue regeneration.

This invention belongs to the field of cosmetic technology and discloses a skin care and hair care liquid composition of water-based polyurethane-10 and type III collagen. This invention combines water-based polyurethane-10 and type III collagen in a specific ratio, utilizing the residual NCO groups (or urea groups, carbamate groups) in polyurethane-10 to form a strong hydrogen bond cross-linking network with the amino and carboxyl groups in collagen molecules. This allows the collagen to firmly adhere to the skin or hair surface, while simultaneously softening the stratum corneum, resulting in a smooth skin feel and achieving multiple effects such as long-lasting moisturizing, improved elasticity, and wrinkle concealing.

The invention relates to the field of biological materials, in particular to collagen hydrogel for filling injection and a preparation method of the collagen hydrogel. The recombinant collagen is dissolved in a citric acid solution to form a collagen and citric acid composite solution, and the concentration of the collagen is 100 mg / mL to 200 mg / mL; heating the composite solution to 80-100 DEG C, reacting for more than 6 hours, dehydrating citric acid molecules to form an anhydride intermediate, and performing esterification reaction on the anhydride intermediate and hydroxyl on a collagen molecular chain to form the three-dimensional network hydrogel taking an ester bond as a main crosslinking bond. A natural metabolite citric acid in an organism is used as a unique cross-linking agent, an active intermediate is formed through intramolecular dehydration under the heating condition of 80-100 DEG C, and the active intermediate and collagen are subjected to a specific esterification reaction to form a covalent cross-linked network. No exogenous chemical cross-linking agent is introduced in the process, so that the risk of toxic cross-linking agent residue is avoided, and the final product has extremely high biocompatibility.

The application provides a preparation kit and a preparation method and application of medical grade type I collagen, and belongs to the technical field of medical and aesthetic polymer materials. The application provides a preparation kit of medical grade type I collagen, which comprises independently packaged chemical degreasing agents, degreasing enzyme solution, swelling liquid, digestive enzyme and purification enzyme. The application realizes physical degreasing, chemical degreasing by using an acetone mixed solution and cooperative degreasing and depilation by using composite enzymes, combines with acid immersion swelling treatment, and maximally retains the triple helix structure of collagen under low-temperature and weak-acid conditions; subsequently, pepsin is used for enzymolysis in a buffer acid system, collagen is released, the extract is subjected to salting-out precipitation and dialysis purification treatment; and terminal peptidase is added to perform enzymolysis on the telopeptide regions at both ends of the collagen molecules, so as to prepare telopeptide-removed collagen, thereby reducing the immunogenicity of the collagen, and improving the biocompatibility and injection safety of the collagen.

The present invention relates to a method for producing collagen-containing anisotropic fibers, the method comprising: a step for bringing an anionic polymer into contact with an aqueous collagen solution in which collagen has been dissolved to form an interface, so that the collagen is formed into fibrils; and a step for pulling up the fibrillated collagen from the aqueous collagen solution with which the anionic polymer has been brought into contact so as to form anisotropic fibers. The present invention also relates to a method for producing a collagen-containing structure having in the interior thereof a hollow structure filled with an aqueous medium, the method comprising: a step for forming, in an aqueous solution containing an anionic polymer, droplets of an aqueous solution in which at least collagen is dissolved; and after the step for forming the droplets, a step for allowing the droplets to stand for a certain period of time to aggregate collagen molecules at the interface between the droplets and the aqueous solution containing the anionic polymer.

The application belongs to the technical field of protein engineering and genetic engineering, and particularly relates to a recombinant type IV humanized collagen, a preparation method and application thereof. A novel recombinant collagen-like protein is obtained through screening, replacement and splicing, and the nucleotide sequence thereof is optimized according to the codon bias of Pichia pastoris, and then a corresponding genetic engineering yeast strain is constructed and obtained, and the recombinant collagen protein is successfully prepared through fermentation. It is proved through tests that the recombinant type IV humanized collagen prepared by the application has large molecular weight and high yield, has better free radical scavenging performance, cell proliferation and migration promotion performance compared with the commercially available recombinant collagen, and has good antioxidant and skin barrier repair functions, and therefore has good practical application value.

The invention discloses a recombinant collagen extracting and filtering device with a backwashing structure, and belongs to the field of recombinant collagen preparation, the recombinant collagen extracting and filtering device comprises a shell, a feed port and a plurality of second discharge ports respectively arranged at the upper end and the lower end of the shell, and a first discharge port arranged outside the shell. According to the scheme, the distribution cavity and the spiral groove are arranged, and when liquid flows in the distribution cavity (the section of the cavity is conical) with the spiral groove, strong rotating flow can be generated due to the constraint of a geometrical shape; according to the invention, liquid enters the material distribution manifold at a certain angular velocity, after the rotational flow with the momentum enters the material distribution manifold, generated turbulent disturbance can effectively wash the inner wall of the manifold, and meanwhile, when the material liquid with the rotational flow residual momentum enters the hollow fiber part, the tangential velocity can generate a similar sweeping effect; and long-chain collagen molecules are prevented from blocking the pipe orifice.

The application discloses low immunogenic collagen and a preparation method and application thereof, and the preparation method comprises the following steps: (1) performing enzymolysis on animal skin; (2) performing salt precipitation treatment on the enzymolysis glue liquid; (3) adding organic amino phosphoric acid or a salt thereof to replace and purify the crude collagen protein prepared through the salt precipitation treatment after redissolving the crude collagen protein, and then performing salt precipitation treatment to obtain purified collagen protein; and (4) redissolving, dialyzing and freeze-drying the purified collagen protein to obtain low immunogenic collagen. Through replacement and removal of DNA impurity molecules on collagen molecular chains by using phosphate in organic amino phosphoric acid or the salt thereof, the amino in the organic amino phosphoric acid or the salt thereof can promote the combination of the phosphate and the collagen under the action of intermolecular hydrogen bonds between the amino and residual carboxyl on the collagen, and the amino and the phosphate play a synergistic effect, so that the removal effect of the DNA impurity in the collagen can be further improved, and the immunogenicity of the collagen is reduced.

The invention is applicable to the technical field of biological materials, and provides recombinant III-type collagen with a transdermal function and application thereof. The recombinant III-type collagen with the transdermal function comprises an oligopeptide segment with skin permeability, a flexible human III-type collagen segment located between the oligopeptide segment and a collagen functional structure segment, and a functional structure segment derived from human III-type collagen. According to the invention, an oligopeptide segment with a transdermal function is introduced into the molecular structure of the III-type collagen, so that the collagen keeps good biocompatibility and structural function, and meanwhile, the whole collagen obtains stable skin penetrating power, thereby expanding the application scene of the collagen in external application such as skin repair, aging resistance and skin care.

The invention provides an irradiation crosslinking collagen injection filler as well as a preparation method and application thereof. The collagen injection filler comprises collagen gel particles, and the collagen gel particles are of a porous structure. The collagen gel particles have connected fiber structures, the fiber structures are formed by collagen self-assembly, and the fiber structures comprise fiber bundles and / or fiber mesh sheet structures; the collagen gel particles in the collagen injection filler have fiber structures formed after collagen self-assembly, that is, collagen is spontaneously assembled from collagen molecules to fiber bundles and fiber mesh lamellar structures, the mechanical property of a fiber network is improved, and the degradation rate is delayed. The fiber network structure provided by the invention has good mechanical support performance and slow degradation rate, simulates a three-dimensional microenvironment of a natural extracellular matrix (ECM), and promotes cell attachment proliferation and tissue regeneration. The fiber structure is beneficial to creeping proliferation of cells and promotes regeneration and repair of skin cells.

This invention belongs to the field of biomedical technology and discloses a non-denatured type I and III collagen ultrafine powder with deep skin growth and three-dimensional repair effects, its molecular protection preparation method, and multiple application pathways. The ultrafine powder contains 50%-70% non-denatured type I collagen, 15%-30% non-denatured type III collagen, 5%-15% molecular protectant, 3%-10% transdermal penetration enhancer, and 2%-8% bioactive stabilizer. The mass ratio of type I to type III collagen is 7:3 to 8:2, the triple helix retention rate is not less than 85%, and the average particle size is 0.5-3 μm. The preparation method employs low-temperature enzymatic extraction and molecular protection complexation technology to achieve complete preservation of the collagen triple helix structure. The product can be applied through surface application, microneedle delivery, and injection to meet the needs of skin repair at different depths.

The invention discloses an oxidized sodium alginate cross-linked collagen suture line and a preparation method thereof. The preparation method comprises the following steps: by taking nutria tail tendons as raw materials, removing immunogenic components by virtue of synergistic treatment of pepsin and trypsin, and cross-linking with an oxidized sodium alginate solution through a Schiff base covalent bond, so as to form the oxidized sodium alginate cross-linked collagen suture line. Oxidized sodium alginate is innovatively adopted as a cross-linking agent, on one hand, Schiff base covalent bonds are formed through aldehyde groups of the oxidized sodium alginate and amino groups of collagen molecules, so that the mechanical property is enhanced, on the other hand, an energy dissipation network is constructed through dynamic interaction among polysaccharide molecules, and the stretching resistance and enzymolysis resistance of the suture line are remarkably improved. The obtained oxidized sodium alginate crosslinked collagen suture line has excellent mechanical performance, degradation resistance and good biocompatibility, and a new strategy is provided for development of absorbable suture materials.

The invention discloses an extraction and activity protection method for functional components in an eggshell membrane. The method comprises the steps of raw material pretreatment and cryogenic superfine grinding, ultrasonic-assisted swelling, compound enzyme step-by-step targeted extraction, separation and purification, multi-source activity protection and the like. The method has the remarkable effects that the method is realized by an integrated technology of copious cooling superfine grinding, ultrasonic assistance, compound enzyme step-by-step targeted extraction and multi-element activity protection, the extraction efficiency of functional components is extremely high, the product activity is excellent, the molecular weight distribution of the extracted collagen is concentrated, and the collagen can be effectively extracted by virtue of a multi-source activity protection measure. The industrial problem that active ingredients are prone to inactivation is systematically solved, the product can be applied to different application scenes such as health care products, cosmetics and biological medicine, and high-value utilization of agricultural waste is achieved.

This invention discloses a feminine care oil capsule based on oil-soluble chimeric collagen and its preparation method, relating to the field of cosmetic technology. The oil phase contents include: 0.5–2.0 wt% oil-soluble chimeric collagen, 95–98 wt% plant oil-based carrier, 0.3–3.0 wt% biomimetic sebum membrane lipid composition, and 0.1–0.5 wt% antioxidant; wherein the biomimetic sebum membrane lipid composition is composed of ceramide, cholesterol, and free fatty acids in a molar ratio of (0.8–1.2):(0.8–1.2):(0.8–1.2). By constructing oil-soluble chimeric collagen molecules, which are stably dispersed in the plant oil-based carrier and self-assembled into nanomicelles, the technical problem that traditional water-soluble collagen cannot be applied to anhydrous oil phase systems is solved. Combined with the scientific formulation of the biomimetic sebum membrane lipid composition, it effectively enhances the vulvar skin barrier repair ability and moisturizing durability. With the specific preparation process and soft capsule dosage form, it achieves high stability of active ingredients, precise dosage, and no preservatives added.

This invention belongs to the field of biomedical material preparation technology, specifically disclosing a method for moist heat sterilization of recombinant collagen fillers. The method includes: preparing a protective solution from modified trehalose and proline, then mixing it with homogenized recombinant collagen hydrogel, followed by staged temperature-controlled moist heat sterilization. This invention modifies trehalose by succinylation, introducing carboxyl groups onto the trehalose molecule, enabling electrostatic interactions with cationic groups on the surface of collagen molecules. During moist heat sterilization, free proline guides the heat-denatured collagen to refold into its natural triple helix structure. The preheating stage allows the collagen to gradually adapt to the thermal environment, avoiding conformational shock damage caused by sudden temperature increases, effectively shortening the high-temperature sterilization time, and significantly reducing the cumulative heat effect on molecular structure. This method is simple to operate, requires no special equipment, and has good prospects for industrial application.

This invention discloses an oral repair membrane and its preparation method, belonging to the field of biomedical materials technology. The invention employs a freeze-thaw process to break down the cell membranes and nuclei within porcine peritoneum tissue, freeing the cell contents for subsequent chemical decellularization. Then, a composite stepwise decellularization system consisting of a first strong alkali wash, a first weak alkali neutralization wash, a second strong alkali wash, and a second weak alkali neutralization wash precisely removes cellular residues, nucleic acids, lipids, and antigenic proteins while maximally preserving the integrity of the collagen structure. Finally, a cross-linking treatment promotes the cross-linking of collagen molecules, significantly improving the mechanical toughness, tensile strength, and in vivo anti-degradation ability of the repair membrane. Combined with a sterilization process, the biosafety of the oral repair membrane is enhanced. The synergistic effect of these steps significantly improves the performance of the prepared oral repair membrane; therefore, this oral repair membrane has promising applications in oral restoration.

The invention discloses collagen-based hydrogel with a fiber spiral entanglement structure and a preparation method of the collagen-based hydrogel, and belongs to the technical field of medical collagen-based hydrogel materials. The preparation method provided by the invention comprises the following steps: after collagen molecules are self-assembled into hydrogel, soaking the hydrogel with polyhydroxy benzaldehyde modified liquid, then carrying out oxidation crosslinking by using an oxidizing agent, and finally cleaning to obtain the product. According to the method, phenolic hydroxyl groups on polyhydroxy benzaldehyde are oxidized into quinone, then the quinone is self-aggregated to form a dimer, covalent cross-linking is constructed among collagen fiber networks, and a unique fiber spiral entanglement structure is formed through induction, so that the prepared collagen-based hydrogel has significantly increased fiber diameter; the mechanical strength, the thermal denaturation temperature and the enzymatic degradation resistance of the hydrogel are far better than those of unmodified hydrogel, meanwhile, the excellent biocompatibility and the cell proliferation promoting capacity are kept, and the hydrogel has the excellent application prospect.

The application provides a preparation method of high-strength collagen sausage casing and relates to the technical field of sausage casing preparation. The preparation method of the high-strength collagen sausage casing comprises the following steps: washing and cutting a cow hide into pieces to obtain hide pieces; adding pepsin to the hide pieces for enzymolysis, inactivation, alkali treatment and acid treatment to obtain hide material; crushing the hide material, filtering and adding a crosslinking agent to obtain mixed slurry. The application adopts a mode of enzymolysis on raw materials and cooperation of glutaraldehyde and plasticizing liquid, first uses glutaraldehyde as a crosslinking agent to react with amino groups in collagen molecules to form a covalent crosslinking bond, and then uses the plasticizing liquid to introduce calcium ions into the hydroxymethyl chitosan to construct a three-dimensional network structure cooperating with the reaction structure of glutaraldehyde, so as to enhance the collagen fiber network structure, improve the mechanical properties of the collagen sausage casing and solve the problem of the decrease of tensile strength caused by the increase of flexibility of the film due to the single addition of glutaraldehyde.

The application relates to the technical field of medical wound dressings, in particular to a bacterial cellulose-collagen in-situ composite dressing film and a preparation method thereof. The preparation method comprises the following steps: preparing a collagen solution and a culture medium; using acetoxybacter and candida as a composite seed solution; under sterile conditions, adding the composite seed solution and the collagen solution into the culture medium, carrying out static fermentation at 29+ / -1 DEG C after sealing, and obtaining a composite film; and carrying out purification treatment on the composite film to obtain the bacterial cellulose-collagen in-situ composite dressing film. Through in-situ composite fermentation, the BC nanofiber forms a continuous three-dimensional skeleton, the collagen molecules are uniformly physically entangled between the fibers, there is no chemical crosslinking agent residue, and the problems of uneven collagen loading and easy falling off can be solved.

The invention discloses an extraction method and application of acidic telopeptide-containing collagen, and relates to the technical field of biological materials.The extraction method comprises the steps that fat of cattle tissue is removed, the cattle tissue is divided into uniform substances, the uniform substances are sequentially soaked with an alkaline solution, a carbonate solution, an organic solvent solution and a salt solution and then drained, and the collagen is obtained; the collagen containing terminal peptide is prepared by means of acid solution soaking extraction, centrifugal impurity removal, acid-base neutralization, centrifugation, secondary acid dissolution, ultrafiltration, microporous filtration, preparation and the like. The terminal peptide-containing collagen with high purity and high terminal peptide retention rate can be prepared; the terminal peptide collagen containing the terminal peptide collagen contains specific amino acid on a crosslinking site and a terminal peptide region, is a key site for forming covalent crosslinking among collagen molecules and inside collagen fibers, has high mechanical strength, and is suitable for the fields of cartilage tissue repair and the like.

The invention relates to the field of biological material preparation, in particular to a collagen microcapsule and a magnetic control directional spinning preparation method thereof, and the method comprises the following steps: S1, S2 and S3, S2, injecting a magnetic collagen stock solution into a modular airflow-assisted spinning device to form microcapsule liquid drops, the modularized airflow auxiliary spinning device comprises a negative pressure generating module and a magnetic field adjusting module. According to the invention, covalent linkage is carried out on the magnetic nanoparticles and collagen molecules, so that the magnetic collagen stock solution can be stably stretched and sheared into microcapsule liquid drops with uniform size and height under the driving of the negative pressure generation module, the consistency of the microcapsule structure is realized, and then through the driving of the magnetic field adjustment module, the magnetic nanoparticles and the collagen molecules can be uniformly dispersed. According to the preparation method, the magnetic nanoparticles can carry collagen molecules to be directionally arranged along the magnetic field direction, so that a highly-ordered microcapsule structure is formed, and finally, the mechanical strength of the collagen microcapsule is improved through crosslinking, so that the collagen microcapsule with excellent performance is prepared.

The present application provides a kind of irradiation crosslinking collagen injection filling agent and its preparation method and purposes.The collagen injection filling agent includes collagen gel microparticle, the collagen gel microparticle has porous structure;The collagen gel microparticle has connected fiber structure, the fiber structure is formed by collagen self-assembly, and the fiber structure includes fiber bundle and / or fiber mesh lamellar structure;The collagen gel microparticle in the collagen injection filling agent of the present application has the fiber structure formed after collagen self-assembly, i.e. collagen is spontaneously assembled from collagen molecule to fiber bundle and fiber mesh lamellar structure, and the degradation rate is delayed.The fiber network structure of the present application has good mechanical support and slow degradation rate, and simulates the three-dimensional microenvironment of natural extracellular matrix (ECM), promotes cell adhesion proliferation and tissue regeneration.The fiber structure of the present application is beneficial to the crawling proliferation of cells, and promotes the regeneration and repair of skin cells.

The present application belongs to the technical field of biological detection, and particularly discloses a cerebral vascular shape real-time detection system based on second harmonic imaging. The present application comprises two parts of a second harmonic imaging device and a software system. A femtosecond two-photon laser scanning microscope is used to perform in-vivo imaging detection on cerebral vessels, and the collagen molecules in the cerebral vessel wall are the detection objects. Experiments prove that the present application uses collagen in the vessel wall as the target molecules, and the blood vessels show a structure of a hollow in the middle and two thin lines on both sides in the imaging, wherein the hollow part is the blood vessel cavity, and the two thin lines are the blood vessel walls on both sides. The present application system is suitable for the detection of living brain, has the advantages of high spatiotemporal resolution, no need for exogenous molecular labeling, and the like, and can detect the cerebral vascular state in real time, quickly and accurately, and has a wide application prospect in the pathological examination of brain in clinical medicine.

The invention relates to a collagen degerming and filtering method. The collagen degerming and filtering method comprises the following steps: 1) dissolving collagen in a solvent containing a strong electrolyte to realize depolymerization; and (2) putting the depolymerized collagen solution into a uniform electric field, and filtering by using a hydrophilic filter membrane with the pore diameter of 0.22 mu m under the constant pressure of 0.1-0.5 MPa. The amphiphilic structure characteristic of collagen molecules is utilized, electrostatic interaction among the molecules is destroyed through a strong electrolyte solution, the collagen is prevented from self-assembling to form a fiber aggregate, and the collagen is stabilized in a monomolecular dispersion state; and the external electric field utilizes the dipole moment characteristic of collagen molecules, so that long axes of the collagen molecules are directionally arranged along the direction of the electric field. Due to directional arrangement, transverse winding of molecules on the surface of the filter membrane is avoided, the problem of blockage caused by molecular aggregation in traditional filtration is fundamentally solved, the service life of a single filter membrane is greatly prolonged, the handling capacity of the single filter membrane is greatly improved, and the cost of filter consumables and the number of times of shutdown replacement are reduced.

The application provides a self-repairing hydroxyapatite / collagen filling material and a preparation method and application thereof. The self-repairing hydroxyapatite / collagen filling material comprises hydroxyapatite and collagen; wherein the collagen is connected by a disulfide bond-containing crosslinking agent between molecules. The application introduces a dynamic disulfide bond to endow the collagen / hydroxyapatite artificial bone with a self-repairing function, so that a temporary balance between digestion and self-repairing is generated in the digestion process, thereby prolonging the digestion speed of the collagen and endowing the collagen with certain mechanical properties.

A method of producing a multifilament collagen fiber bundle involves treating a multifilament support polymer-collagen fiber bundle containing a plurality of collagen monofilaments supported on a support polymer to remove the support polymer from the multifilament support polymer-collagen fiber bundle using a series of liquid media containing a decreasing concentration of the support polymer. The support polymer is more soluble than collagen in a liquid medium, and the gradual removal of the support polymer provides sufficient time for collagen molecules to self-assemble to form fibrils. The resulting multifilament collagen fiber bundle has a D-band structure similar to native collagen, but is offset by 1 / 6 D-band. The multifilament collagen fiber bundle can be cross-linked through ultraviolet rays under the condition of no photoinitiator to form a cross-linked collagen product with excellent tensile strength and Young modulus, and the product is suitable for collagen-based biological materials in the field of tissue repair and regeneration.

The invention relates to the technical field of biological materials, and particularly discloses PDRN-crosslinked collagen gel and a preparation method thereof.According to the gel, through a TCFH-NMI system mediated amidation reaction, polydeoxyribonucleotide (PDRN) and a collagen molecular chain are subjected to covalent crosslinking, and a stable three-dimensional network structure is formed. The preparation method comprises the following steps: respectively dissolving collagen and PDRN, then mixing, adding NMI, adding TCFH in batches, carrying out a cross-linking reaction, and after the reaction is finished, carrying out dialysis purification to obtain the PDRN-cross-linked collagen gel. The prepared gel has good structural stability, mechanical performance and biocompatibility, can effectively delay PDRN release and improve the in-vivo stability of the material, and is suitable for the fields of wound repair, skin tissue engineering scaffolds, medical beauty filling materials and the like.

The application discloses a room temperature phosphorescent collagen composite film based on network synergistic enhancement and a preparation method and application thereof. The preparation method comprises the following steps: firstly, grafting phosphorescent molecules containing amino groups to the side chain of collagen molecules through an amide reaction; then, introducing a polymer containing hydroxyl groups and layered nanosheets into a collagen film forming solution; finally, fully combining the polymer and the layered nanosheets with the collagen matrix through non-covalent interactions such as hydrogen bonds by a solvent evaporation method to prepare the room temperature phosphorescent collagen composite film based on network synergistic enhancement. The preparation method is simple and easy to operate, raw materials are widely sourced and safe and environmentally friendly; the semi-rigid network of the polymer in the film and the network enhancement effect and oxygen blocking effect of the layered nanosheets can synergistically enhance the room temperature phosphorescent performance of the film, so that the film has high mechanical properties, self-supporting properties and processability, and therefore, the film has great application potential in the fields of anti-counterfeiting, information encryption, flexible sensing, intelligent labels and the like.

The invention relates to the technical field of genetic engineering, in particular to stable recombinant XVII type collagen and application thereof. The stable recombinant XVII type collagen has a stable molecular structure, the molecular weight of the stable recombinant XVII type collagen is smaller than that of a complete sequence, and the stable recombinant XVII type collagen contains functional active sites and can give consideration to transdermal absorption and biological structure stability; the stable recombinant XVII type collagen can realize industrial production, and has good application in the fields of medicines, medical equipment, biological materials, tissue engineering products, cosmetics or health care products and the like.

The invention discloses a diphasic collagen implant as well as a preparation method and application thereof, and belongs to the technical field of medical instruments, the diphasic collagen implant is composed of collagen molecular gel and collagen fiber gel; the content of collagen in the dual-phase collagen implant is 20 to 65 mg / mL; the collagen fiber gel accounts for 45%-85% of the total mass of the dual-phase collagen implant. The biphasic collagen implant disclosed by the invention is relatively good in comprehensive mechanical property and relatively good in water retention property, and can provide relatively good moisturizing and moistening effects for skin tissues. Besides, the dual-phase collagen implant maintains the maintenance time similar to that of single collagen fiber gel, the treatment time efficiency of the dual-phase collagen implant is not shortened, and the application range and the treatment effect of the dual-phase collagen implant in the field of medical beauty are widened.