142 results about "Collagen molecule" patented technology

The invention provides a high-strength collagen base artificial bone repair material which can be used for repairing the bone defect of bearing parts of a human body. The high-strength collagen base artificial bone repair material has chemical composition and structure formed by self-assembly of nanometer calcium phosphate and collagen molecules, so that the high-strength collagen base artificial bone repair material has a biomimetic mineralization structure similar to the natural bone tissue of the human body. In terms of mechanical properties, the material has the mechanical strength similar to that of the cortical bone of the human body and can be used for repairing the bone defect of the bearing parts of the human body. The invention also provides a preparation method of the high-strength collagen base artificial bone repair material.

The invention discloses a meso-porous silicon nano-drug carrier with both reduction responsiveness and targeting ability and a preparation method thereof. Firstly, meso-porous silicon nano-particles are prepared with a sol-gel method, and are developed into a nanometer reservoir of drug molecules; then a disulfide bond is introduced into the surface of the meso-porous silicon nanometer reservoir with a simple chemical modification method and is used as a cohesive tie; secondarily, a biocompatible natural extracellular matrix-collagen molecule is creatively fixed to the surface of the meso-porous silicon nanometer reservoir and is developed into a nanometer encapsulator of the meso-porous silicon nanometer reservoir; and finally a lactobionic acid molecule is modified to the surface of a meso-porous silicon / collagen nanometer compounding system and is used as a specificity receptor of a liver cancer cell membrane surface receptor (ASGP-R) so as to construct a meso-porous silicon / collagen-lactobionic acid multifunctional compound type nano-drug carrier system with both cell specificity targeting ability and reducing substance / enzyme responsiveness.

The invention relates to an oral nutrition composition for beautifying skin, which contains collagen, hyaluronicacid and vitamin C, wherein the collagen is active small molecule peptide with the molecular weight of 2000-6000Dalton, the hyaluronicacid is sodium salt, zinc salt and kali salt, and the vitamin C is natural or synthesized. The invention overcomes the defects that the collagen can not be absorbed by a human body or hydrolyzed into amino acid because the molecular weight is too big, and most of the collagen loses the beautifying efficacy and the like. Hydroxyproline in collagen peptide is promoted to generate by using the vitamin C through active prolyl hydroxylases, the collagen forms big molecule collagen required by beautifying the skin by crosslinked with the hydroxyproline, the hyaluronicacid is also a better nutrient absorption promoting agent except for special water-holding function and is matched with the vitamin C and the collagen for use, which can achieve more ideal effect of promoting the hyaluronicacid to be absorbed.

The present invention provides a preparation method of high-strength bionic collagen membrane. The preparation method is by conducting gradient dialysis process on collagen to rearrange collagen molecules into an ordered structure, and endow the molecules with more consistent and more extensive collagen fiber orientation, thus improving the mechanical properties of the material. The collagen membrane prepared by using the method has similar appearance, structure and performance to natural dura and amnion of mammals, and good rehydration, and is free to bend and fold after water absorption; the bionic collagen membrane with high tensile strength can be stitched by using suture and can be used in clinical guided tissue regeneration or repair of various tissues.

The invention discloses an injectable type collagen-based soft tissue filling material and preparation method thereof, wherein the filling material mainly comprises collagen 0.5-20%, heparin 0.1%-10%, cell growth factor 0-1%, cushion liquid 70-99.4%, and its preparation comprises extracting soluble collagen from animal connective tissue, cutting off antigen deciding family from the collagen molecules through proteinase hydrolysis, purifying treatment, crosslinking by utilizing safety and nontoxic crosslinking agent, charging biologically active components, kneading the mixture repeatedly into grease form, sealing under asepsis condition and storing at low temperature.

The invention relates to an edible collagen sausage-casing produced by a gelatinized peel, pertains to the sausage-casing packaging material use for meat food processing and production method thereof. Based on collagen molecular construction theory, the method gelatinizing peels from different source or peel from the same source but with different quality with acid, alkali or enzyme to produce uniform slurry, forms and dries to obtain uniform, stabilized collagen sausage-casing with no obvious quality difference, and solves difference between materials from different source and application and quality of obtained product for materials from the same material. The sausage-casing produced by the method has high pressure resistant strength, high humidity and heat resistant pulling force, great adhesiveness of meat paste, no break phenomenon caused by over-contracting, and characteristic of chewing easiness in sausage production, so that the method is suitable for production of various kinds of sausages.

The invention relates to a cationic flocculant based on collagen modification and a preparation method thereof. The cationic flocculant is prepared through the following steps of: 1) taking trypsin as hydrolytic agent to hydrolyze industrial gelatine to obtain collagen solution; 2) using glyoxylic acid or gallic acid to modify the obtained collagen solution aiming at increasing the content of carboxyl groups or phenolic hydroxyl groups on collagen molecules to obtain modified collagen solution; and 3) conducting complexation to the obtained modified collagen solution and ferric sulfate or aluminum sulfate according to mass ratio being 1:2 to 1:4 to obtain the cationic flocculant based on the collagen modification. The cationic flocculant can be used for treating waste drilling fluid and solves the problems that the preparation cost of the existing flocculant is high, the synthesized or degraded products are highly toxic and carcinogenic and serious environmental pollution is caused during the current treatment of the waste drilling liquid.

The invention discloses a method for extracting micromolecular collagen oligopeptide from fish, comprising the following step of: lipase degreasing: putting cleaned and dried scales or fishskin into an extraction pot; evenly mixing by adding water according to weight of 3-15 times; carrying out zymohydrolysis by adding lipase according to 0.01-1 percent of the weight of the scales or the fishskin; and then, cleaning, regulating a pH value, carrying out zymohydrolysis by adding the prolease, blanching, filtering, concentrating and drying to obtain the micromolecular collagen oligopeptide. The prolease comprises the following zymohydrolysis step of: carrying out zymohydrolysis by adding the prolease according to 1-4 percent of the weight of the scales or the fishskin for 1-6 hours at the temperature of 38-68 DEG C. Because the lipase is adopted to degrease the scales or fishskin, the method increases degreasing effect, thereby greatly reducing zymohydrolysis time of the prolease and improving production efficiency; and moreover, the processed collagen has lower molecular weight and is beneficial to being absorbed by a human body.

The invention discloses a method for extracting collagen from crucian carp skin. The method comprises steps of preprocessing, washing, enzymatic extraction, salting out, dialysis and freeze drying. The method has the following beneficial effects: the method adopts simple preparation steps andrequires a small quantity of devices, accurate data is provided for each step of operation, the reproducibility is good, and the method is suitable for large-scale production; the collagen is prepared from the crucian carp skin, namely, the crucian carp processing leftover as a raw material, the cost is low, the rate of utilization of the crucian carp is increased, and added value of the crucian carp is increased; the prepared collagen is high in biological activity and can be applied to the fields of drugs, food, health care products, cosmetics and the like; the speed for extracting the collagen with an enzymatic hydrolysis method is high, non-helical telopeptide can be selectively removed by pepsin, so that collagen molecules can be dissolved out, the triple helix structure of the collagen is retained, and the biological activity of the collagen is maintained.

The invention relates to a method for preparing microfiber hemostatic collagen sponge. The method comprises the following steps of: aggregating collagen molecules by the charge effect by adjusting pH, temperature and the salt concentration of collagen protein solution, freeze-drying the obtained and assembled collagen, then preparing into the collagen protein sponge, and then carrying out thermal crosslinking to obtain the microfiber hemostatic collagen sponge. The microfiber hemostatic collagen sponge prepared by adopting the method has uniform porous structure, good hydrophilic performance, high mechanical strength and degradation resistance and good hemostatic performance, and the defects that the traditional collagen sponge is easy in degradation, poor in mechanical property, easy in collapse in the application process and difficult in maintenance of the inherent form and the like are solved. The preparation method is simple and feasible, and is stable in process, and the prepared microfiber hemostatic collagen sponge can be widely applied in hemostasis and restoration of wounds of burns, traumas and surgical operation, and has good application prospect.

Provided is a collagen structure characterized by: comprising collagen fibers of 1 to 5 µm in average diameter; and has a water content of 0 to 15 (w / w)% and a collagen density of 50 to 800 mg / cm 3 . After generating collagen fibers by neutralizing an acidic collagen solution, the resulting solution is subjected to filtration or the like to form crude collagen fibers having a collagen concentration of 12 to 50 (w / v)%. The thus obtained crude collagen fibers are molded into a prescribed shape and then dried, thereby the collagen structure can be produced. Since the collagen structure is produced using, as raw material, collagen fibers that are formed by association of collagen molecules, the collagen structure has excellent cell infiltration property. Further, since the collagen density of the collagen structure is equivalent to that of in vivo collagen tissue, the collagen structure exhibits excellent tissue regeneration capacity when filled into a defective part in vivo. Therefore, the collagen structure can be preferably used as an artificial material for regenerative medicine and the like.

The invention discloses a high-purity natural collagenous fiber and a preparation method thereof. The collagenous fiber is characterized in that fresh traceable animal muscle tendon or achilles tendon is used as a raw material, and is subjected to pre-treatment, water soaking, supercritical carbon dioxide fluid degreasing, alkali leaching, first enzyme treatment, acid leaching, secondary enzyme treatment, salting out, centrifugal purification and other processes to obtain the high-purity natural collagenous fiber. The high-purity natural collagenous fiber prepared by the method is aggregation of natural collagen molecules, has the quarternary structure of collagen and the intrinsic bioactivity of collagen, and has more spatial structure features of collagen in natural tissues, so that the natural tissue structure can be further simulated. Compared with a collagen material, the natural collagenous fiber prepared by the method has the advantages of excellent mechanical performance, thermal stability and biodegradation performance, and the structure of the natural collagenous fiber is relatively stable and easy to store; and characteristic group expression of cells can be activated, and normal characteristic expression of cells can be maintained, so that adhesion, growth and proliferation of cells can be benefited. The natural collagenous fiber can be used for preparing hemostatic materials, tissue engineering scaffold materials, medical plastic and cosmetic materials and the like.

The invention relates to biomaterial compositions and methods for promoting bone regeneration and hemostasis. The invention also relates to compositions and methods for promoting wound healing. In various embodiments, the compositions comprise crosslinkable collagen molecules and calcium phosphate suitable for bone regeneration. In various embodiments, the compositions comprise crosslinkable collagen molecules suitable for promoting hemostasis or wound healing; or suitable as tissue sealants. In some embodiments, the compositions contain additional agents, including biological agents.

This invention relates to a collagen polypeptide comprising a tripeptide motif having the formula (ProYaaGly)n, where Yaa is an O-methylated amino acid residue and “n” is the number of motif repeats. Preferred O-methylated amino acid residues at the Yaa position include (2S,4R)-4-methoxyproline. Other suitable amino acid residues at that position include O-mono or O-di-halogenated methylproline. Also, disclosed is a method of making a synthetic or a semi-synthetic collagen polypeptide molecule having increased stability relative to natural collagen. The strengthened collagen molecules are suitable for use in biomaterials for the medical field or in leather-related products prepared by the tanning industry.

The invention discloses a preparation method of a tannin-loaded collagen / cellulose composite biological adsorption material. The method comprises the following steps of dissolving cellulose powder in ion liquid which is adopted as a medium, adding sponge-shaped collagen fibers, dissolving the sponge-shaped collagen fibers, and uniformly mixing the sponge-shaped collage and the cellulose; regenerating and washing the ion liquid containing the cellulose / collagen to obtain a composite material; placing the composite material into an aqueous solution, adding plant tannin, stirring to facilitate the reaction, filtering and washing the unreacted tannin, and adding a cross-linking agent to have reaction; filtering and washing the unreacted cross-linking agent, and freeze drying the reaction product to obtain the tannin-loaded collagen / cellulose composite biological adsorption material. The cellulose is used as a substrate for fixinng little collagen, and the plant tannin is further loaded on the collagen molecules, so that the capacity of the material for adsorbing metal ions can be achieved. Metal ions can be high-efficiently and selectively adsorbed, the stability of the material is also enhanced, and the obtained composite material can be used for treating industrial waste water containing heavy metal ions such as lead and copper.

The invention relates to an extracellular matrix freeze-drying protection liquid. The extracellular matrix freeze-drying protection liquid takes normal saline as a solvent and comprises the following components: chondroitin sulfate, vitamin C, polyethylene glycol, trehalose, glucan, serine and threonine. The invention has the following advantages: the protection liquid has high penetrability, can be vitrified under a low-temperature condition, is rich in negative polar groups and hydroxyl groups, and can be efficiently bonded with collagen molecules; the stable and uniform extracellular matrix freeze-drying effect can be ensured; the protection liquid can be formed into the vitreous state at low temperature, thereby preventing ice crystals from damaging the protein structure; and a protection film is formed at the outer layer of the matrix in stead of hydrogen bonds formed by water molecules and collagen, thereby protecting the collagen function and the stability of the three-dimensional structure. The extracellular matrix freeze-drying protection liquid has low cytotoxicity, and can be used immediately after being rinsed with normal saline or sterilized water; the freeze-dried extracellular matrix can be preserved at normal temperature for more than three years when being packaged in vacuum; and the extracellular matrix freeze-drying protection liquid is suitable for the preservation of purified extracellular matrix such as collagen, elastin, glucoprotein and the like, and is also suitable for various biologic materials constructed by extracellular matrix materials.

The invention discloses a manufacturing process of an artificial collagen sausage casing. The process comprises the steps of collagen preparing, collagen molecular filling material adding, and sausage casing forming. The collagen preparing step comprises the processes of: animal skin sorting, sterilizing, washing, manual skin cutting, crushing, stirring, acidifying, enzymolysis, secondary washing, and fiber-removing treating. When the manufacturing process of the artificial collagen sausage casing is implemented, artificial collagen sausage casings with advantages of good safety, good sanitation, good air permeability, uniform thickness, and non-significant heat-shrinkage can be produced.

A biofabricated material containing a network of crosslinked collagen fibrils is disclosed. This material is composed of collagen which is also a major component of natural leather and is produced by a process of fibrillation of collagen molecules into fibrils, crosslinking the fibrils and lubricating the crosslinked fibrils. Unlike natural leathers, this biofabricated material exhibits non-anisotropic (not directionally dependent) physical properties, for example, a sheet of biofabricated material can have substantially the same elasticity or tensile strength when stretched or stressed in different directions. Unlike natural leather, it has a uniform texture that facilitates uniform uptake of dyes and coatings. Aesthetically, it produces a uniform and consistent grain for ease of manufacturability. It can have substantially identical grain, texture and other aesthetic properties on both sides distinct from natural leather where the grain increases from one side (e.g., distal surface) to the other (proximal inner layers).

The invention discloses a processing technology of cowhide leather. The technology comprises the steps of: cleaning, degreasing, hair removal, alkali dipping, deashing, softening, acid dipping and tanning. According to the invention, the ester removal agent used in degreasing treatment can remove the residual ester well, is beneficial to intracutaneous penetration of tanning agent molecules and combination with collagen molecule active groups in a follow-up tanning process, formation of a cross-linked protein fiber three-dimensional network, and improvement of the stability and chemical resistance, and the prepared leather does not rot or deteriorate easily. Through the treatment of alkali dipping, deashing, softening and other steps, mesenchymal protein fiber in cortex can be dissolved, the softness of finished leather can be improved, and the handfeel is comfortable.

A biofabricated material containing a network of crosslinked collagen fibrils is disclosed. This material is composed of collagen which is also a major component of natural leather and is produced by a process of fibrillation of collagen molecules into fibrils, crosslinking the fibrils and lubricating the crosslinked fibrils. Unlike natural leathers, this biofabricated material exhibits non-anisotropic (not directionally dependent) physical properties, for example, a sheet of biofabricated material can have substantially the same elasticity or tensile strength when stretched or stressed in different directions. Unlike natural leather, it has a uniform texture that facilitates uniform uptake of dyes and coatings. Aesthetically, it produces a uniform and consistent grain for ease of manufacturability. It can have substantially identical grain, texture and other aesthetic properties on both sides distinct from natural leather where the grain increases from one side (e.g., distal surface) to the other (proximal inner layers).

Described, in certain aspects, are medical products comprised of uniquely prepared remodelable extracellular matrix (ECM) materials retaining at least a portion of their native bioactivity. Also described are methods for forming and using such products. In one embodiment, an inventive product comprises a layer of remodelable ECM material modified through contact with periodic acid or a salt thereof. In some forms, such a modified ECM material layer includes non-native Schiff's base crosslinks within and / or between certain components of the ECM material (e.g., between two collagen molecules, two non-collagen molecules, and / or a collagen molecule and a non-collagen molecule). Other inventive products are comprised of various gels, foams, pastes, and formed, coherent, porous bodies at least containing ECM materials that have been modified in accordance with the present invention.

The collagen base nano coloring pigment with low infrared emissitivity is in core-shell structure with inner core of nano metal oxide coloring pigment and outer collagen grafting copolymer. Collagen molecule is first adsorbed onto the surface of nano pigment particle to modify and stabilize the nano particle, lipophilic grafting branch chain is then introduced to the collagen molecule via solution grafting copolymerization under the action of initiator and the collagen grafting copolymer coats the coloring pigment. The collagen base nano coloring pigment has relatively low infrared emissivity and good dispersivity in organic solvent or water, and it has pigment particle size smaller than 100 nm and outer shell thickness less than 20 nm. It is used in preparing stealth coating and stealth costume and can realize visible light stealth and infrared stealth without damaging radar stealth.

The invention relates to a method for crosslinking collagen through an oxidized oligosaccharide. The method comprises the steps that the oxidized oligosaccharide with a certain quantity of aldehyde groups serves as a crosslinking agent, a crosslinking reaction occurs between the oxidized oligosaccharide and collagen molecules or an aggregate of the collagen molecules, then cleaning or dialyzing treatment is performed, and finally freeze drying is performed with a freeze drying machine to obtain crosslinked collagen molecules containing the oligosaccharide structure or an aggregate of the crosslinked collagen molecules. According to the crosslinked collagen obtained through the method, not only are the original performances of collagen kept, but also the oligosaccharide structure with the activity is introduced, the formed crosslinked structure can resist a certain degradation function of biological enzymes, all the basic performances are excellent, the biocompatibility is good, and the certain functionality is simultaneously achieved. The method can be applied to biological material and leather industries.

The invention relates to a collagen sponge with a recombined fiber structure and a preparation method of the collagen sponge. The collagen sponge has a recombined fiber structure, the whole structure is in a sponge shape, and the surface of the collagen sponge has a collagen microfiber structure. Collagen molecules exist in a fiber manner and have a three-dimensional helical structure. The preparation method mainly comprises: purifying and concentrating collagen through tangential flow filtering technology, performing in-vitro molecular self-assembly, performing centrifugation, gelating, and repeated pre-freezing, unfreezing and washing, and performing freeze drying. The preparation method is high in production power and stable in process. The prepared collagen sponge with a recombined fiber structure can be widely applied to hemostasis and restoration of trauma and surgical wounds, and has bright application prospects.

The invention relates to the technical field of casing production, in particular to a production process of a boiled / fried collagen casing. In the process of extracting collagen fibers, small molecular polyols, a strong oxidant and ferric ions are added into a semi-finished product after ash washing to carry out pretreatment, and then acidification is carried out. The method performs pretreatmenton the semi-finished product after ash washing, increases the crosslinking degree in the collagen casing; a collagen group is obtained by adding proper auxiliary materials and collagen fibers; the casing blowing process is improved by optimizing the process parameters and adjusting the process steps; and the crosslinking further occurs among collagen molecules and in the molecules by optimizing the technological parameters of a sleeve shrinkage curing process. The process is simple and feasible; the prepared casing has good temperature resistance, pressure resistance and mechanical properties;and the casing is uniform and beautiful in color, stable in quality and beneficial to industrial production.

The present invention provides a compression-resistant collagen-based artificial bone repair material that could be used for bone defect repair at human load-bearing sites. Such material has a composition and structure of self-assembled nano-sized calcium phosphate salt and collagen molecules, thereby possessing a biomimetic mineralization structure similar to the natural bone. On the aspect of mechanical properties, such material has a similar mechanical strength to human cortical bone, which makes it suitable for repairing bone defects at human load-bearing sites. The present invention further provides preparation methods of such Compression-resistant collagen-based artificial bone repair material.

A biofabricated material containing a network of crosslinked collagen fibrils is disclosed. This material is composed of collagen which is also a major component of natural leather and is produced by a process of fibrillation of collagen molecules into fibrils, crosslinking the fibrils and lubricating the crosslinked fibrils. Unlike natural leathers, this biofabricated material exhibits non-anisotropic (not directionally dependent) physical properties, for example, a sheet of biofabricated material can have substantially the same elasticity or tensile strength when stretched or stressed in different directions. Unlike natural leather, it has a uniform texture that facilitates uniform uptake of dyes and coatings. Aesthetically, it produces a uniform and consistent grain for ease of manufacturability. It can have substantially identical grain, texture and other aesthetic properties on both sides distinct from natural leather where the grain increases from one side (e.g., distal surface) to the other (proximal inner layers).

The invention discloses a method for retanning leather by using bioenzyme, and in particular relates to reinforcement of a spatial structure of collagen by using the bioenzyme. The method is applied to a retanning process after a main tanning process. In the retanning process, transglutaminase is applied to the catalyzing of crosslinking in molecules and between the molecules of the collagen, so that physical and chemical indexes such as thermal stability, tear strength, fullness and the like of the leather are further improved. An application process comprises the following steps of: adding a certain amount of bioenzyme, namely 0.5 to 2U of bioenzyme, into every gram of dry blue leather which is tanned by the conventional process at the bath ratio of 1:0.6-1:0.9, the temperature of between 30 and 40 DEG C and the pH value of between 5.0 and 8.0; and treating in a revolving drum for 60 minutes. The tear strength, the fullness and the extensibility of the treated leather are correspondingly improved compared with those of untreated raw leather.

The invention discloses a method for preparing a cornea lamina material. The method comprises the following steps of: carrying out accellular processing on a cornea-sclera complex of a mammal eyeball, culturing an amnion epithelial stem cell, inducing and secreting TSP (Thrombospondin)-1 and co-culturing accellular cornea stroma and the amnion epithelial stem cell, sizing, packaging and sterilizing, and the like. According to an obtained cornea lamina transplanting material, the promotion and the balance among the biocompatibility, the stability, the transparency, the mechanical strength and the bioactivity are realized; the integrity of cornea collagen molecules and a lamina structure can be kept to the largest extent, and compared with that prepared with the traditional method, the accellular cornea stroma prepared according to the method disclosed by the invention is larger in tensile strength and has the moisture content and the transparency close to those of normal cornea; and various cell growth factors and the TSP-1 are gathered, and the cornea lamina material has special effects of inflammatory resistance and vascularization resistance, has the capability of greatly enhancing the bioactivity of the cornea lamina material and can be widely used for repairing complicated ocular surface coloboma such as inflammation and corneal ulcer grown in a vascularization way.