The invention discloses a high-purity natural collagenous
fiber and a preparation method thereof. The collagenous
fiber is characterized in that fresh traceable animal
muscle tendon or
achilles tendon is used as a
raw material, and is subjected to pre-treatment, water soaking,
supercritical carbon dioxide fluid
degreasing, alkali leaching, first
enzyme treatment, acid leaching, secondary
enzyme treatment,
salting out, centrifugal purification and other processes to obtain the high-purity natural collagenous
fiber. The high-purity natural collagenous fiber prepared by the method is aggregation of natural collagen molecules, has the quarternary structure of collagen and the intrinsic bioactivity of collagen, and has more
spatial structure features of collagen in natural tissues, so that the natural tissue structure can be further simulated. Compared with a collagen material, the natural collagenous fiber prepared by the method has the advantages of excellent mechanical performance,
thermal stability and
biodegradation performance, and the structure of the natural collagenous fiber is relatively stable and easy to store; and characteristic group expression of cells can be activated, and normal characteristic expression of cells can be maintained, so that adhesion, growth and proliferation of cells can be benefited. The natural collagenous fiber can be used for preparing hemostatic materials,
tissue engineering scaffold materials, medical plastic and cosmetic materials and the like.