9 results about "Collagen molecule" patented technology

This invention belongs to the field of cosmetic technology and discloses a skin care and hair care liquid composition of water-based polyurethane-10 and type III collagen. This invention combines water-based polyurethane-10 and type III collagen in a specific ratio, utilizing the residual NCO groups (or urea groups, carbamate groups) in polyurethane-10 to form a strong hydrogen bond cross-linking network with the amino and carboxyl groups in collagen molecules. This allows the collagen to firmly adhere to the skin or hair surface, while simultaneously softening the stratum corneum, resulting in a smooth skin feel and achieving multiple effects such as long-lasting moisturizing, improved elasticity, and wrinkle concealing.

The application provides a preparation kit and a preparation method and application of medical grade type I collagen, and belongs to the technical field of medical and aesthetic polymer materials. The application provides a preparation kit of medical grade type I collagen, which comprises independently packaged chemical degreasing agents, degreasing enzyme solution, swelling liquid, digestive enzyme and purification enzyme. The application realizes physical degreasing, chemical degreasing by using an acetone mixed solution and cooperative degreasing and depilation by using composite enzymes, combines with acid immersion swelling treatment, and maximally retains the triple helix structure of collagen under low-temperature and weak-acid conditions; subsequently, pepsin is used for enzymolysis in a buffer acid system, collagen is released, the extract is subjected to salting-out precipitation and dialysis purification treatment; and terminal peptidase is added to perform enzymolysis on the telopeptide regions at both ends of the collagen molecules, so as to prepare telopeptide-removed collagen, thereby reducing the immunogenicity of the collagen, and improving the biocompatibility and injection safety of the collagen.

The present invention relates to a method for producing collagen-containing anisotropic fibers, the method comprising: a step for bringing an anionic polymer into contact with an aqueous collagen solution in which collagen has been dissolved to form an interface, so that the collagen is formed into fibrils; and a step for pulling up the fibrillated collagen from the aqueous collagen solution with which the anionic polymer has been brought into contact so as to form anisotropic fibers. The present invention also relates to a method for producing a collagen-containing structure having in the interior thereof a hollow structure filled with an aqueous medium, the method comprising: a step for forming, in an aqueous solution containing an anionic polymer, droplets of an aqueous solution in which at least collagen is dissolved; and after the step for forming the droplets, a step for allowing the droplets to stand for a certain period of time to aggregate collagen molecules at the interface between the droplets and the aqueous solution containing the anionic polymer.

The application discloses low immunogenic collagen and a preparation method and application thereof, and the preparation method comprises the following steps: (1) performing enzymolysis on animal skin; (2) performing salt precipitation treatment on the enzymolysis glue liquid; (3) adding organic amino phosphoric acid or a salt thereof to replace and purify the crude collagen protein prepared through the salt precipitation treatment after redissolving the crude collagen protein, and then performing salt precipitation treatment to obtain purified collagen protein; and (4) redissolving, dialyzing and freeze-drying the purified collagen protein to obtain low immunogenic collagen. Through replacement and removal of DNA impurity molecules on collagen molecular chains by using phosphate in organic amino phosphoric acid or the salt thereof, the amino in the organic amino phosphoric acid or the salt thereof can promote the combination of the phosphate and the collagen under the action of intermolecular hydrogen bonds between the amino and residual carboxyl on the collagen, and the amino and the phosphate play a synergistic effect, so that the removal effect of the DNA impurity in the collagen can be further improved, and the immunogenicity of the collagen is reduced.

This invention belongs to the field of biomedical technology and discloses a non-denatured type I and III collagen ultrafine powder with deep skin growth and three-dimensional repair effects, its molecular protection preparation method, and multiple application pathways. The ultrafine powder contains 50%-70% non-denatured type I collagen, 15%-30% non-denatured type III collagen, 5%-15% molecular protectant, 3%-10% transdermal penetration enhancer, and 2%-8% bioactive stabilizer. The mass ratio of type I to type III collagen is 7:3 to 8:2, the triple helix retention rate is not less than 85%, and the average particle size is 0.5-3 μm. The preparation method employs low-temperature enzymatic extraction and molecular protection complexation technology to achieve complete preservation of the collagen triple helix structure. The product can be applied through surface application, microneedle delivery, and injection to meet the needs of skin repair at different depths.

This invention discloses a feminine care oil capsule based on oil-soluble chimeric collagen and its preparation method, relating to the field of cosmetic technology. The oil phase contents include: 0.5–2.0 wt% oil-soluble chimeric collagen, 95–98 wt% plant oil-based carrier, 0.3–3.0 wt% biomimetic sebum membrane lipid composition, and 0.1–0.5 wt% antioxidant; wherein the biomimetic sebum membrane lipid composition is composed of ceramide, cholesterol, and free fatty acids in a molar ratio of (0.8–1.2):(0.8–1.2):(0.8–1.2). By constructing oil-soluble chimeric collagen molecules, which are stably dispersed in the plant oil-based carrier and self-assembled into nanomicelles, the technical problem that traditional water-soluble collagen cannot be applied to anhydrous oil phase systems is solved. Combined with the scientific formulation of the biomimetic sebum membrane lipid composition, it effectively enhances the vulvar skin barrier repair ability and moisturizing durability. With the specific preparation process and soft capsule dosage form, it achieves high stability of active ingredients, precise dosage, and no preservatives added.

This invention belongs to the field of biomedical material preparation technology, specifically disclosing a method for moist heat sterilization of recombinant collagen fillers. The method includes: preparing a protective solution from modified trehalose and proline, then mixing it with homogenized recombinant collagen hydrogel, followed by staged temperature-controlled moist heat sterilization. This invention modifies trehalose by succinylation, introducing carboxyl groups onto the trehalose molecule, enabling electrostatic interactions with cationic groups on the surface of collagen molecules. During moist heat sterilization, free proline guides the heat-denatured collagen to refold into its natural triple helix structure. The preheating stage allows the collagen to gradually adapt to the thermal environment, avoiding conformational shock damage caused by sudden temperature increases, effectively shortening the high-temperature sterilization time, and significantly reducing the cumulative heat effect on molecular structure. This method is simple to operate, requires no special equipment, and has good prospects for industrial application.

The application provides a preparation method of high-strength collagen sausage casing and relates to the technical field of sausage casing preparation. The preparation method of the high-strength collagen sausage casing comprises the following steps: washing and cutting a cow hide into pieces to obtain hide pieces; adding pepsin to the hide pieces for enzymolysis, inactivation, alkali treatment and acid treatment to obtain hide material; crushing the hide material, filtering and adding a crosslinking agent to obtain mixed slurry. The application adopts a mode of enzymolysis on raw materials and cooperation of glutaraldehyde and plasticizing liquid, first uses glutaraldehyde as a crosslinking agent to react with amino groups in collagen molecules to form a covalent crosslinking bond, and then uses the plasticizing liquid to introduce calcium ions into the hydroxymethyl chitosan to construct a three-dimensional network structure cooperating with the reaction structure of glutaraldehyde, so as to enhance the collagen fiber network structure, improve the mechanical properties of the collagen sausage casing and solve the problem of the decrease of tensile strength caused by the increase of flexibility of the film due to the single addition of glutaraldehyde.

The application relates to the technical field of medical wound dressings, in particular to a bacterial cellulose-collagen in-situ composite dressing film and a preparation method thereof. The preparation method comprises the following steps: preparing a collagen solution and a culture medium; using acetoxybacter and candida as a composite seed solution; under sterile conditions, adding the composite seed solution and the collagen solution into the culture medium, carrying out static fermentation at 29+ / -1 DEG C after sealing, and obtaining a composite film; and carrying out purification treatment on the composite film to obtain the bacterial cellulose-collagen in-situ composite dressing film. Through in-situ composite fermentation, the BC nanofiber forms a continuous three-dimensional skeleton, the collagen molecules are uniformly physically entangled between the fibers, there is no chemical crosslinking agent residue, and the problems of uneven collagen loading and easy falling off can be solved.