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30 results about "Protein retention" patented technology

In general, protein retention in the Golgi can perhaps best be viewed as a dynamic process in which one or more features of a Golgi resident protein contributes, to varying degrees, to its steady-state retention in a particular subcompartment.

Stabilized proteins

InactiveUS7037894B2Prevent undesired cross-linkControl cross-link reactionHormone peptidesPeptide/protein ingredientsTyrosineProtein retention
Isolated polypeptides or polypeptide chains are modified by di-tyrosine cross-linking such that the retain at least one functional activity. In one embodiment, the isolated polypeptide or polypeptide chains comprise at least one di-tyrosine cross-link, wherein at least one tyrosine of the di-tyrosine cross-link originates from a point mutation to tyrosine, and wherein the di-tyrosine cross-linked protein retains at least one function displayed by the protein in the absence of di-tyrosine cross-linking. In another embodiment, the di-tyrosine cross-linked polypeptide or polypeptide chain has enhanced stability compared to the same polypeptide or polypeptide chain in the absence of di-tyrosine cross-linking. A method for stabilization of a polypeptide or polypeptide complex, by the introduction of intra-polypeptide and / or inter-polypeptide di-tyrosine bonds, which simultaneously maintains the structure and function of the polypeptide or polypeptide complex is also described.
Owner:CALDER BIOSCI INC

Peanut polypeptide liquid and peanut protein polypeptide beverage prepared from the peanut polypeptide liquid

The invention discloses peanut polypeptide liquid and a peanut protein polypeptide beverage prepared from the peanut polypeptide liquid. A preparation method of the peanut polypeptide liquid comprises the following steps of drying peanut kernels by an oven, removing red skin, carrying out ultra-fine crushing to obtain peanut butter, adding the peanut butter into hot water having a temperature above 75 DEG C for dissolution according to a weight ratio of 1: (7 to 27), adjusting a pH value to of the peanut butter solution to a pH value of 7.0 to 8.5, based on the weight of the peanut butter, adding 0.3 to 0.9 wt% of peanut composite protease into the peanut butter solution, carrying out enzymolysis at a temperature of 45 to 65 DEG C for 0.5 to 2.5 hours, deactivating the peanut composite protease, and filtering to obtain the peanut polypeptide liquid. The preparation method of the peanut polypeptide liquid is simple, saves time and has a low cost. Dry ultra-fine crushing is utilized in raw material treatment so that a peanut protein retention ratio is high and an enzymolysis speed is fast. The peanut composite protease is utilized in proteolysis so that a degree of hydrolysis, the concentration of a trichloroacetic acid soluble protein, and a polypeptide yield are high. The peanut protein polypeptide beverage prepared from the peanut polypeptide liquid, white sugar and peanut protein liquid prepared from peanut butter which is subjected to dry ultra-fine crushing and is dissolved in water has the advantages of rich nutrient, specific fragrance belonging to peanut, smooth andfine mouthfeel, and no bitter taste.
Owner:SOUTHWEST UNIVERSITY +1

Compositions and methods for proteomic investigations

Abstract of the Disclosure The present invention provides a variety of related proteomics analytical modalities that are open-ended, rapid, convenient and suitable for implementation in a high throughput parallel assay system. Specificity-determining compositions and methods are disclosed for use in proteomics. These compositions and methods provide a protein resolved from other protein species contained in a sample fluid, in its native, biologically functional conformation. The present invention provides a specificity-determining substrate that forms a complex with a protein molecule in a homogenous fashion. The specificity-determining substrate includes a specificity-determining ligand bound to a support, wherein optionally the substrate further includes a spacer bound between the ligand and the support. In addition a complex is provided that includes a specificity-determining substrate and a protein molecule. Furthermore, an array including a plurality of loci is provided, in which each locus includes a specificity-determining substrate of the invention. These substrates, complexes and arrays may be employed in a method of resolving a first protein from a fluid including one or more species of native, biologically active protein molecules, wherein the first protein retains its native structure and its biological activity; in a method of purifying one or more first proteins from a fluid including one or more species of native, biologically active protein molecules, wherein the purified first protein retains its native structure and its biological activity; in a method of characterizing one or more proteins in a fluid including one or more species of protein molecule; and in a method of identifying one or more proteins in a sample fluid wherein the concentration of the one or more proteins in the sample fluid differs from the concentration of the one or more proteins in a reference fluid.
Owner:ROY SWAPAN +2

Gene of human vascular endothelial growth factor monoclonal antibody and use thereof

The invention relates to heavy chain and light chain variable region genes of a monoclonal antibody of a human vascular endothelial growth factor, a polypeptide that is encoded by the genes, a carrier that contains the genes, and application of the genes and the polypeptide in the preparation of a clinical testing agent of VEGF-related tumors. The invention utilizes gene engineering means to firstly obtain the heavy chain and light chain variable region genes of the monoclonal antibody from a hybridoma cell strain of the monoclonal antibody with VEGF165 secretion, the heavy chain variable region gene has the nucleotide sequence with SEQ ID NO of 1, the light chain variable region gene has the nucleotide sequence with SEQ ID NO of 3, and the genes are combined with scFv small molecule antibody and cloned into a pET28a expression carrier, have high-efficiency expression in colon bacillus and generate protein that has reserved antibody combination capability and VEGF165 action function. Through the modification with gene engineering techniques, the invention establishes the foundation for biological missile construction, has ultra-convenient antibody production and greatly lowered cost, and is favorable for industrial production.
Owner:林植华

Process for producing functional non-naturally occurring proteins, and method for site-specific modification and immobilization of the proteins

There is provided a process for industrial production of non-naturally occurring proteins composed of less than 20 amino acids, wherein the proteins retain their original functions while being capable of site-specific modification or immobilization, or having new functions not found in nature in addition to the original functions of the proteins.
Specifically there are provided a process for producing a functional non-naturally occurring protein having a specific amino acid type(s) replaced with a natural amino acid(s) other than the amino acid type(s), the process comprising:
    • a) matching a nucleic acid portion having a nucleotide sequence reflecting the genotype with a protein portion that is the translation product of the nucleic acid portion;
    • b) selecting the matched molecule obtained in step a);
    • c) introducing mutation into the nucleic acid portion of the matched molecule obtained in step b);
    • d) amplifying the nucleic acid portion obtained in step c);
    • e) providing the nucleic acid portion obtained in step d) to step a), to match the nucleic acid portion with a protein portion that is the translation product of the nucleic acid portion; and
    • f) selecting the matched molecule obtained in step e), to produce a functional non-naturally occurring protein;
    • and a method for site-specific modification and site-immobilization of a functional non-naturally occurring protein.
Owner:WASEDA UNIV

Preparation method of biological feed through reinforced denaturation of wine brewing industrial by-products

The invention relates to a preparation method of a biological feed through reinforced denaturation of wine brewing industrial by-products. The preparation method comprises the following steps of mixing the wine brewing industrial by-products with wheat bran, tapioca, traditional Chinese medicine powder and milk powder; and uniformly spraying activated bacillus FY99-01 strains and a compound enzymepreparation in mixed materials, performing aerobic fermentation, and after fermentation is completed, adding anaerobic strains so as to obtain the denaturation feed. According to the making technology disclosed by the invention, crude protein which cannot be absorbed in by-products can be converted into micromolecular water-soluble protein, so that absorption by intestinal tracts of livestock andpoultry can be promoted, protein retention in excrement can be substantially reduced, the balanced compounding ratio of amino acids and leucine in materials can be improved, absorption of nutrient substances by the livestock and the poultry can be promoted, the problems of higher cellulose expansion coefficient and a certain bonding degree of the by-products are solved, and digestion and absorption of nutrients are promoted.
Owner:海南汇萃生物科技有限公司

Preparation method of peanut peptide

The invention belongs to the technical field of peanut protein processing, and particularly relates to a preparation method of peanut peptide. The preparation method of the peanut peptide comprises the following steps of: S1) preparing peanut protein isolate; S2) performing ultrasonic enzymolysis treatment to obtain enzymatic hydrolysate; S3) performing electrodialysis desalination: adding the enzymatic hydrolysate obtained in the step S2 into a fresh water chamber of an electrodialysis device, adding 8-12g / L of a sodium sulfate aqueous solution into an electrode water chamber, adding a compound solution into a concentrated water chamber, applying 20-30V direct current voltage to electrodes on two sides of the electrodialysis device, and stopping when the conductivity is reduced to 100 microseconds / cm; and S4) performing drying: collecting feed liquid in the fresh water chamber in the step S3, and performing drying to obtain the peanut peptide. According to the preparation method disclosed by the invention, peanut meal is sequentially subjected to the steps of alkali extraction, acid extraction, ultrasonic assisted enzymolysis and electrodialysis desalination to obtain the peanut peptide, so that the protein retention rate of the peanut peptide is greatly increased, and the peanut peptide is good in antioxidant activity.
Owner:GUANGZHOU TIANQI BIO TECH
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