30 results about "Protein retention" patented technology

The present invention provides an artificial scaffolding material for retaining proteins suitable for placing contiguously one species or two or more species of proteins such as enzymes. To this end, the artificial scaffolding material for retaining proteins is provided with a cell and scaffolding proteins heterologous to the cell and placed on the surface layer side of the cell at an extent that allows aggregation properties to be conferred to the cell, and provided with a plurality of non-covalently binding protein-binding domains arranged in tandem.

The invention discloses a method for preparing inositol from a corn steep liquor. The processes comprise clarification and separation, ultrafiltration and filtration, mashing, hydrolysis, neutralization, filtration, ion exchange, concentration, decolorization, cooling crystallization, drying and refining, so as to give a finished product. According to the method, the protein retention rate in a ultrafiltration membrane separation process is high; calcium phytate in form of aqueous paste is adopted in the mashing process, a high hydrolysis speed and a high yield are achieved due to crystal water provided by calcium phytate, and the yield of inositol can be increased by direct mashing; the addition of alkaline earth metal carbonate in the hydrolysis process can promote complete hydrolysis and increase the yield of inositol, and a feeding sequence also enables a reaction of the hydrolysis process to be complete; and in the ion exchange process, the adoption of a cation-anion resin can improve the crystallinity of inositol and reduce the mother liquor amount, thereby improving the purity of the inositol product.

A casing for containment of a proteinaceous product during or following processing is described. The casing has controlled porosity which permits close control of product drying with protein retention, providing good product color and surface texture, patterned and/or shaped products, high protein content and high peelability. The casing is a hollow tubular laminate of an outer lamina having high strength and, optionally, surface pattern-producing capability, and an inner laminate of controlled porosity which is permeable by vapors and, in most cases, also aqueous and organic liquids, but which is not permeable to solids such as proteins or fats. The laminae are adhered by an adhesive (preferably thermally activated) or by surface interlocking. Accessory materials such as liquid flavorants, colorants, release agents and anti-microbial agents can be incorporated into the laminae and from there imparted to the product. Applicable proteinaceous products include meat, poultry, seafood, soya and cheese.

Abstract of the Disclosure The present invention provides a variety of related proteomics analytical modalities that are open-ended, rapid, convenient and suitable for implementation in a high throughput parallel assay system. Specificity-determining compositions and methods are disclosed for use in proteomics. These compositions and methods provide a protein resolved from other protein species contained in a sample fluid, in its native, biologically functional conformation. The present invention provides a specificity-determining substrate that forms a complex with a protein molecule in a homogenous fashion. The specificity-determining substrate includes a specificity-determining ligand bound to a support, wherein optionally the substrate further includes a spacer bound between the ligand and the support. In addition a complex is provided that includes a specificity-determining substrate and a protein molecule. Furthermore, an array including a plurality of loci is provided, in which each locus includes a specificity-determining substrate of the invention. These substrates, complexes and arrays may be employed in a method of resolving a first protein from a fluid including one or more species of native, biologically active protein molecules, wherein the first protein retains its native structure and its biological activity; in a method of purifying one or more first proteins from a fluid including one or more species of native, biologically active protein molecules, wherein the purified first protein retains its native structure and its biological activity; in a method of characterizing one or more proteins in a fluid including one or more species of protein molecule; and in a method of identifying one or more proteins in a sample fluid wherein the concentration of the one or more proteins in the sample fluid differs from the concentration of the one or more proteins in a reference fluid.

Use of an plant materials, plant extracts or nature-identical components selected from Lonicera japonica, Gentiana asclepidea, Gentiana lutea, Eugenia caryophyllata, Bellis perennis, Olea europaea, Symphytum officinale, Carduus pycnocephalus, Paeoniae alba radix, Populus tremula, Prunus avium, Salix caprea, Rheum nobile, Helianthemum canum, Arctostaphylos uva-ursi, Peltiphyllum peltatum, Epilobium montanum, Knautia arvensis, Latuca sativa and Urtica dioica and extracts thereof, and beta-myrcene to affect the rumen fermentation and to increase the availability of energy and nitrogen source for a ruminant.

The invention discloses a cellulose acetate/chitosan blood dialysis membrane and a preparation method thereof, and relates to the field of biomaterials. The cellulose acetate/chitosan blood dialysis membrane is a hollow fiber film with hollow fiber diameter of 0.4-4 microns, aperture sizes of 2.2-4.8 microns and wall thickness of 40-80 microns. The dialysis membrane is prepared from 33-56 parts by mass of cellulose acetate, 12-18 parts by mass of chitosan, 5-10 parts by mass of phosphoric acid, 3-10 parts by mass of polyvinyl alcohol, 12-22 parts by mass of tetrahydrofuran and 8-16 parts by mass of dimethyl acetamide. The preparation method comprises 1, spinning liquid preparation and 2, electrostatic spinning. The cellulose acetate/chitosan blood dialysis membrane has good biocompatibility and good biofunctionability, has a high macromolecular protein retention rate and a high urea and water passing rate, and is an ideal dialysis membrane material.

The present invention relates to human B lymphocyte exciting factor monoclonal antibody heavy chain and light chain variable region gene, the gene coded polypeptide, vector containing the gene, the application of the gene and the polypeptide in preparing clinical detecting reagent for BLyS relative diseases and autoimmune diseases, and the preparation process. The heavy chain variable region gene has full length of 342 bp, nucleotide sequence shown in <400>1, and coded amino acid shown in <400>3; and the light chain variable region gene has full length of 321 bp, nucleotide sequence shown in <400>2, and coded amino acid shown in <400>4. The gene engineering expression produced protein has the antibody combining ability maintained and small scFv molecule, is easy to combine with other effector molecule via gene engineering process. The present invention lays foundation for constituting biological missile, has low cost and is favorable to industrial production.

Provided herein is a means for increasing protein retention in blood. The protein retention in blood is increased by forming a conjugate of a protein and chondroitin produced by a microorganism having chondroitin-producing capability and/or chondroitin synthesized with a chondroitin synthase.

The invention discloses a preparing method of protein concentrate of sacha inchi fruits. By using the characteristics that the dissolving rates of the protein of sacha inchi fruits and micromolecule impurities in an ethanol solution and an acid aqueous solution are different, sacha inchi fruit meal is extracted with an ethanol solution, alcohol soluble impurities are separated, precipitation is extracted with acid water, acid water soluble impurities are separated, the precipitation is added with water to regulate the pH to be neutral, and the protein concentrate of sacha inchi fruits can be obtained through high-pressure homogenization and spray drying. The preparing method of the protein concentrate of sacha inchi fruits is low in cost, and the protein concentrate is white in color, free of acerbity, edible, high in yield and protein retention rate and capable of achieving mechanization.

The combination of a supported UF membrane having low non-specific binding (NSB) and high protein retention of the tested chemical entity (CE) in a device that is SBS complaint. The membrane is heat sealed to form one or more integral wells that are used to reduce NSB and improve protein retention and provides a simple, flexible way to reduce CE, such as drug and drug candidate (and other small molecule) NSB so that drug binding studies may more closely predict the behavior of these compounds in vivo.

The invention discloses a method and device for carrying out protein purification on the basis of isoelectric point difference of proteins. A principle of the method and the device is as follows: during electrophoresis, a target protein hardly swims in a buffer solution, of which isoelectric points are the same as those of the target protein. Impurity proteins swim to the positive pole or negative pole of an electrophoresis bath due to negative charges or positive charges and are concentrated on electrode bath areas, so as to be separated from the target protein. A sample is isolated from positive and negative pole areas of the electrophoresis bath by adopting a microporous material, so that the target protein can be reserved in a sample pool after electrophoresis. Meanwhile, the buffer solution in an electrode bath is uninterruptedly updated during electrophoresis, so that the condition that the swimming of the impurity proteins stops and the impurity proteins and polarized accumulated ions are taken away due to pH polarization and the concentration polarization of various charged ions is avoided.

The invention discloses a fermentation medium containing waste bean curd yellow pulp and waste bean dregs and its application. Each liter of the fermentation medium includes the following components: 500 mL-1000 mL of treated waste bean curd yellow pulp, The final treated waste okara is 60-80 g, the inorganic salt is 0.25-30 g, and the pH is 6.5-7.5. The present invention adopts the combination treatment method of enzymatic hydrolysis and ceramic membrane filtration to treat waste bean curd yellow slurry water, and can obtain protein retention rate of 78%~85%, and total sugar penetration rate of 92%~96%; A large amount of cellulose in the fermentation process is hydrolyzed into glucose, which is the carbon source required for the fermentation of Corynebacterium glutamicum. The present invention can utilize the wastes in production such as bean curd yellow pulp water and bean dregs, which can not only reduce the cost of industrial production, avoid the waste of waste resources, but also reduce the pollution of waste materials to the environment, and at the same time increase the production of glutamic acid. The biomass of Corynebacterium acid and the production of L-ornithine.