A kind of fermentation medium comprising waste bean curd yellow pulp water and waste bean dregs and its application

A technology for fermentation medium and yellow slurry water, applied in the biological field, can solve the problems of imperfect setting of conditions, loss of beneficial substances, too simple treatment method, etc., and achieve the effects of reducing industrial costs, less pollution, and reducing environmental pollution.

Active Publication Date: 2022-04-22
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Of course, there are also some people who have processed waste tofu yellow pulp and waste bean dregs, and then added them to the culture medium, but due to some processing methods are too simple and the conditions are not perfect, etc., resulting in some beneficial effects on the process of fermentation and amino acid production. material loss
In addition, different strains require different nutrients and some small organic molecules in the process of fermentation and amino acid production. There are no reports about Corynebacterium glutamicum using waste tofu yellow pulp and waste bean dregs as components of the fermentation broth.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 This embodiment illustrates the treatment of waste tofu yellow slurry water

[0040] In this embodiment, the treatment of tofu yellow pulp water first adopts centrifugation to remove useless dregs and sediments in the yellow pulp water. The centrifugal speed is 4000r / min. different. By adding the water supernatant of tofu yellow pulp to control the temperature, the temperature is controlled between 40 ℃ and 48 ℃, the pH of the waste tofu yellow pulp water supernatant is adjusted to 6-7, and 0.8% transglutaminase is added for enzymatic The enzymatic reaction time was 45 min. The JWCM19*30 series 50 nm inorganic membrane was used, and the filtration pressure was 1 Mpa. The cross-flow method was used to concentrate the protein in the tofu yellow slurry water, and the obtained filtrate was the treated waste tofu yellow slurry water. Such a temperature will not cause damage to its amino acids and organic acids, and has a better effect on the subsequent filtrat...

Embodiment 2

[0042] Embodiment 2 This embodiment illustrates the treatment of waste bean dregs

[0043] 1) Grind and homogenize the fresh bean dregs pulled back from the tofu factory to make them in a uniform state;

[0044] 2) Use excess KOH with a concentration of 1mol / L to treat the homogeneous bean dregs, add KOH while stirring, add 1 mol / L sulfuric acid to adjust to neutral after treatment, rinse the bean dregs with distilled water, and rinse 3 times;

[0045] 3) Dry the wet bean dregs in an oven at 65°C, and collect the dried bean dregs after drying for 6 hours;

[0046] 4) Grind and sieve the dried bean dregs to obtain finer grains of dried bean dregs powder, take a certain amount of dried bean dregs and dissolve them in water, the amount of bean dregs is 25g / L, and treat it with 72% concentrated sulfuric acid in 5 , reflect in 80℃ water bath for 30min, decompose cellulose into glucose and other small molecular substances, add alkali to adjust the pH to neutral after treatment, and...

Embodiment 3

[0049] Recipe 1: 1L of fermentation medium, including the following components: 500 mL of untreated waste bean curd yellow slurry water, 40 g of untreated waste bean dregs, K 2 HPO 4 0.8 g, KH 2 PO4 0.8 g, MgSO 4 ·7H 2 O 0.25 g, CaCO 3 30g, add water to make up to 1L, pH 7.

[0050] Formula 2: Use 1 L of untreated waste tofu yellow slurry water as the culture substrate, add 40 g of untreated waste bean dregs, K 2 HPO 4 0.8 g, KH 2 PO4 0.8 g, MgSO 4 ·7H 2 O 0.25 g, CaCO 3 30g, pH 7.

[0051] Recipe 3: Use 1 L of untreated waste tofu yellow slurry water as a culture substrate, add 80 g of untreated waste bean dregs, K 2 HPO 4 0.8 g, KH 2 PO4 0.8 g, MgSO 4 ·7H 2 O 0.25 g, CaCO 3 30g, pH 7.

[0052] Recipe 4: In 1L of fermentation medium, the following components are included: 500 mL of waste tofu yellow slurry water after treatment, 40 g of waste bean dregs after final treatment, K 2 HPO 4 0.8 g, KH 2 PO4 0.8 g, MgSO 4 ·7H 2 O 0.25 g, CaCO 3 30g, pH 7, ...

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Abstract

The invention discloses a fermentation medium containing waste bean curd yellow pulp and waste bean dregs and its application. Each liter of the fermentation medium includes the following components: 500 mL-1000 mL of treated waste bean curd yellow pulp, The final treated waste okara is 60-80 g, the inorganic salt is 0.25-30 g, and the pH is 6.5-7.5. The present invention adopts the combination treatment method of enzymatic hydrolysis and ceramic membrane filtration to treat waste bean curd yellow slurry water, and can obtain protein retention rate of 78%~85%, and total sugar penetration rate of 92%~96%; A large amount of cellulose in the fermentation process is hydrolyzed into glucose, which is the carbon source required for the fermentation of Corynebacterium glutamicum. The present invention can utilize the wastes in production such as bean curd yellow pulp water and bean dregs, which can not only reduce the cost of industrial production, avoid the waste of waste resources, but also reduce the pollution of waste materials to the environment, and at the same time increase the production of glutamic acid. The biomass of Corynebacterium acid and the production of L-ornithine.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a fermentation medium containing waste bean curd yellow pulp water and waste bean dregs and an application thereof. Background technique [0002] Waste tofu yellow pulp water mainly comes from the wastewater leached during tofu processing and production, which contains rich organic matter. Nowadays, most food processing enterprises directly discharge the tofu yellow slurry water produced in the process of tofu processing from the sewer, which pollutes the natural environment and wastes effective resources. Due to the huge soybean product industry in my country, it will inevitably lead to the discharge of a large amount of waste tofu water, which is not conducive to the development of my country's green ecology. Therefore, if the waste tofu yellow pulp water can be effectively utilized in the production process of soybean products, it can not only reduce industrial costs ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P13/10C12R1/15
CPCC12N1/20C12P13/10
Inventor 郝宁冯宇静郭格格黄彤舸单咸旸徐大春欧阳平凯
Owner NANJING UNIV OF TECH
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