Toxicology experiment device and method for evaluating toxicity of pesticide by using toxicology experiment device

A technology of experimental device and evaluation method, which can be used in material inspection products, testing pharmaceutical preparations, etc., can solve the problems of complex detection process and unstable reliability of detection results, and achieve the effect of high reliability and simple structure.

Inactive Publication Date: 2021-07-23
DONGGUAN UNIV OF TECH
View PDF10 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the technical defects of the above-mentioned prior art that the detection process is relati...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Toxicology experiment device and method for evaluating toxicity of pesticide by using toxicology experiment device
  • Toxicology experiment device and method for evaluating toxicity of pesticide by using toxicology experiment device
  • Toxicology experiment device and method for evaluating toxicity of pesticide by using toxicology experiment device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 is a 10-day sediment acute toxicity test. In this experiment, the lethality of third-instar chironomus larvae and the inhibition rate of acetylcholinesterase (AChE) in sediments containing harmful components such as pesticides were used as the basic indicators for evaluating the toxicity of pesticides. According to the Bt protein concentration of different samples of sediments, the experimental The subjects were divided into 5 groups, and there were 3 control groups: a blank control group, a solvent control control group and a negative control group. Blank control is to carry out toxicological experiment without adding any compound; Solvent control control group is to only add the solvent (for example ethanol or acetone) of dissolving target compound to beaker and carry out toxicological experiment, the purpose is to examine the influence of solvent on test organism; and The negative control group is to add the protein solution extracted from ordinary cotton, ...

Embodiment 2

[0063] Embodiment 2 is a 4-day water acute toxicity test. The basic principle and steps of embodiment 2 are the same as in embodiment 1, but the difference is that the time of the experiment is only 4 days, and the main substrate is a water body. During the experiment, it is not necessary to change the water in the beaker. The experiment includes the following steps:

[0064] Step 1: After stirring the sediment samples to be tested, divide them into 5 groups and divide them into 300mL beakers. Add 10g of treated clean sand to each beaker, then add 200mL of water samples, and let stand After a period of time (usually standing overnight) until the upper layer of water is clarified; another set of blank control, a solvent control control group and a negative control group are set.

[0065] Step 2, add 10 third-instar chironomus larvae into each beaker.

[0066] Step 3: Control the experimental temperature at 23±1°C, and keep the light / dark ratio of 16 / 8.

[0067] Step 4: Feed 1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a toxicological experiment device and a method for evaluating pesticide toxicity by using the toxicological experiment device. The toxicological experiment device comprises an experiment bin, a controller, a water inlet pipe and a water outlet pipe, a plurality of beakers are arranged in the experiment bin, and an automatic electronic valve is arranged on each branch pipeline for communicating the water inlet pipe with one beaker; a drain hole with a filter screen is formed in the side wall of the lower edge of the opening end of each beaker; the drain hole of each beaker is communicated through a branch pipeline water pipe 104; an automatic electric heating device is arranged at the bottom of the experiment bin; and a temperature sensor is also arranged in the experiment cabin. According to the pesticide toxicity evaluation method, the toxicity experiment device is used as main experiment equipment, chironomidae larvae are used as experiment objects, and the fatality rates of the Bt proteins with different concentrations on the chironomidae larvae are used as main indexes to evaluate the pesticide toxicity. The device is simple in structure, and the reliability of the pesticide toxicity evaluation method is high.

Description

technical field [0001] The invention belongs to the technical field of pesticide toxicity detection, and in particular relates to a toxicological experiment device and a method for evaluating pesticide toxicity applied thereto. Background technique [0002] There are many existing devices and methods for pesticide toxicity detection or evaluation. For example, utility model patent No. 202020916785.8 discloses a pesticide toxicity analysis operation protection device, including a test bench, an operating table, a protective box, and a hinge. And the sealed door, the bottom of the operating table is fixed on the top of the test bench, the bottom of the protective box is fixed on the top of the experimental bench, and the operating table is located inside the protective box. The pick-and-release port is matched with the rotating connection, and there is a handle on the airtight door; it also includes the fan, the fan base, the connecting pipe, the suction port, the fixing piece...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/15
CPCG01N33/15
Inventor 李衍亮游静苏城远胡俊杰吕小梅范洪波刘美杰邱芷莘卜少腾徐祎
Owner DONGGUAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap