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Culture medium capable of quickly and accurately detecting listeria monocytogenes and detection method

A technology of Listeria monocytogenes and culture medium, applied in the field of bacterial detection, can solve the problems of high cost of culture medium, long detection period, poor identification effect, etc., and achieve the effect of shortening the detection period

Inactive Publication Date: 2018-03-23
番禺出入境检验检疫局综合技术服务中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a fast and accurate detection culture medium and detection method for Listeria monocytogenes, to solve the problems of expensive culture medium for detection of Listeria monocytogenes, poor identification effect and long detection period in the prior art

Method used

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  • Culture medium capable of quickly and accurately detecting listeria monocytogenes and detection method
  • Culture medium capable of quickly and accurately detecting listeria monocytogenes and detection method
  • Culture medium capable of quickly and accurately detecting listeria monocytogenes and detection method

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Experimental program
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Effect test

Embodiment 1

[0031] 1.1 Preparation of medium

[0032] The ingredients of each 1000mL medium include: soybean lecithin 2.5g, agar 15g, tryptone 10g, glucose 1g, soybean peptone 4g, dipotassium hydrogen phosphate 2g, yeast powder 6g, beef heart powder 2g, sodium chloride 5g, chloride Lithium 1g, activated carbon powder 2.5g.

[0033] Agar, tryptone, soy peptone, yeast powder, beef heart powder, LB 1 The former enrichment solution was produced by Beijing Land Bridge Technology Co., Ltd., the sodium chloride was from Tianjin Dingshengxin Chemical Co., Ltd., the soybean lecithin was from Guangdong Huankai Microbial Technology Co., Ltd., and the lithium chloride was from Tianjin Fengchuan Chemical Reagent Technology Co., Ltd. Activated carbon was from Shanghai Aladdin Biochemical Technology Co., Ltd., glucose and dipotassium hydrogen phosphate were from Guangzhou Chemical Reagent Factory, and Listeria culture solution was from SY-LAB Company.

[0034] Culture medium preparation steps:

[003...

Embodiment 2

[0046] 2.1 Preparation of medium

[0047] Each 1000mL medium composition includes: soybean lecithin 2.5g, agar 15g, tryptone 10g, glucose 1g, soybean peptone 4g, dipotassium hydrogen phosphate 2g, yeast powder 6g, beef heart powder 2g, sodium chloride 5g, chloride Lithium 1g, activated carbon powder 3g.

[0048] The source of materials is the same as in Example 1.

[0049] Culture medium preparation steps:

[0050] (1) First add 2.5 g of soybean lecithin into 50 mL of distilled water for sterilization;

[0051] (2) Dissolve agar 15g, tryptone 10g, glucose 1g, soybean peptone 4g, dipotassium hydrogen phosphate 2g, yeast powder 6g, beef heart powder 2g, sodium chloride 5g, lithium chloride 1g, activated carbon powder 3g in 950mL distilled water;

[0052] (3) After autoclaving the ingredients prepared in step (2) at 121°C for 15 minutes, adjust the pH to 7.3,

[0053] (4) After cooling down to 45°C, add 50 mL of the soybean lecithin solution prepared in step (1) aseptically, m...

Embodiment 3

[0058] 3.1 Preparation of medium

[0059] The ingredients of each 1000mL medium include: soybean lecithin 2.5g, agar 15g, tryptone 10g, glucose 1g, soybean peptone 4g, dipotassium hydrogen phosphate 2g, yeast powder 6g, beef heart powder 2g, sodium chloride 5g, chloride Lithium 1g, activated carbon powder 5g.

[0060] The source of materials is the same as in Example 1.

[0061] Culture medium preparation steps:

[0062] (1) First add 2.5 g of soybean lecithin into 50 mL of distilled water for sterilization;

[0063] (2) Dissolve agar 15g, tryptone 10g, glucose 1g, soybean peptone 4g, dipotassium hydrogen phosphate 2g, yeast powder 6g, beef heart powder 2g, sodium chloride 5g, lithium chloride 1g, activated carbon powder 5g in 950mL distilled water;

[0064] (3) After autoclaving the ingredients prepared in step (2) at 121°C for 15 minutes, adjust the pH to 7.3,

[0065] (4) After cooling down to 45°C, add 50 mL of the soybean lecithin solution prepared in step (1) asepti...

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Abstract

The invention discloses a culture medium capable of quickly and accurately detecting listeria monocytogenes and a detection method. The disclosed culture medium comprises the following components: soya bean lecithin, agar, Casein tryptone, glucose, soy peptone, dipotassium phosphate, yeast powder, beef heart powder, sodium chloride, lithium chloride, active carbon powder and distilled water. The detection method comprises the following steps: preparing the components into the culture medium according to a method, then selecting a sample to firstly carry out LB1 pre-enrichment and listeria monocytogenes enrichment and then inoculate the culture medium prepared in the invention, after the culture is finished, selecting bacterial colonies generating settling rings, and carrying out purification and bacterium identification according to the national standard GB4789.30-2016. The culture medium has the main advantages that the settling rings for listeria monocytogenes appear at early time, and the detection time is shortened. The detection rate, specificity and false positive rate are equivalent to those in national standard, and the listeria monocytogenes can be detected effectively. According to the detection method disclosed by the invention, the period is greatly shortened compared with the national standard, and the method is free of significant difference.

Description

technical field [0001] The invention relates to the technical field of bacterial detection, in particular to the identification culture medium and detection technology of Listeria monocytogenes. Background technique [0002] The current national standard and international standard procedure for the detection of Listeria monocytogenes is LB 1 Pre-enrichment 24 hours, LB 2 After 24 hours of bacterial enrichment, the suspicious colony is isolated from the streaked chromogenic medium and then biochemically identified, which generally takes 4-7 days. The principle of separation and identification of chromogenic medium is: according to the production of β-D-glucosidase glucosidase in the tested bacteria to distinguish whether it is Listeria, and then according to the presence or absence of lecithinase phospholipase to distinguish Listeria monocytogenes from Inno Listeria kleistia, Listeria wieseri, Listeria stunneri, and Listeria gasseri (Listeria is capable of producing phospho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/01
CPCC12Q1/045G01N2333/195
Inventor 李太存周清芳许纯刘二龙王乔笙
Owner 番禺出入境检验检疫局综合技术服务中心
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