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a culture medium

A technology of basal medium and complete medium, applied in microorganism-based methods, instruments, biochemical equipment and methods, etc., can solve the problems of only 50-70% positive rate, low positive rate, insufficient sensitivity, etc.

Active Publication Date: 2021-06-22
SHENZHEN BLOT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The culture of Helicobacter pylori (H.Pylori, HP) is a very important technical means for diagnosing the infection of Helicobacter pylori. However, the biggest defect in the culture of HP at present is the low sensitivity. The positive rate of general culture is only 50-70%. Even under the sampling and culture of very experienced laboratories and operators, the positive rate can barely Increase to 70-90% (with immunohistochemistry as the reference standard)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Medium formula for cultivating Helicobacter pylori:

[0059] Each 1000mL agar plate contains:

[0060]

[0061] Concrete preparation steps:

[0062] By recipe:

[0063]

[0064] After mixing, heat to dissolve, then filter with filter paper, adjust the pH value to 7.2±0.1, dispense 800mL / bottle, autoclave in a high-pressure steam sterilizer, sterilize at 121°C for 20min, cool to room temperature and seal, 4°C Save for later.

[0065] When preparing plates:

[0066] 1) Take a certain amount of basal medium stored at above 4°C, add agar powder at an amount of 25g agar powder per 1L, and then sterilize under high pressure at 121°C for 20 minutes;

[0067] 2) When the temperature of the culture medium drops to about 45°C-50°C, add the stock solution of various antibiotics according to the concentration of 12mg vancomycin, 6mg amphotericin, 3mg polymyxin and 3mg trimethoprim per 1L medium ;

[0068] 3) Add fresh sheep blood according to the amount of 100mL fresh s...

Embodiment 2

[0072] Medium formula for cultivating Helicobacter pylori:

[0073] Each 1000mL agar plate contains:

[0074]

[0075] Concrete preparation steps:

[0076] By recipe:

[0077]

[0078]

[0079] After mixing, heat to dissolve, then filter with filter paper, adjust the pH value to 7.2±0.1, dispense 800mL / bottle, autoclave in a high-pressure steam sterilizer, sterilize at 121°C for 20min, cool to room temperature and seal, 4°C Save for later.

[0080] When preparing plates:

[0081] 1) Take a certain amount of basal medium stored at above 4°C, add agar powder at an amount of 25g agar powder per 1L, and then sterilize under high pressure at 121°C for 20 minutes;

[0082] 2) When the temperature of the culture medium drops to about 45°C-50°C, add the stock solution of various antibiotics according to the concentration of 10mg vancomycin, 5mg amphotericin, 5mg polymyxin and 5mg trimethoprim per 1L medium ;

[0083] 3) Add fresh sheep blood according to the amount of 1...

Embodiment 3

[0087] Medium formula for cultivating Helicobacter pylori:

[0088] Each 1000mL agar plate contains:

[0089]

[0090]

[0091] Concrete preparation steps:

[0092] By recipe:

[0093]

[0094] After mixing, heat to dissolve, then filter with filter paper, adjust the pH value to 7.2±0.1, dispense 800mL / bottle, autoclave in a high-pressure steam sterilizer, sterilize at 121°C for 20min, cool to room temperature and seal, 4°C Save for later.

[0095] When preparing plates:

[0096] 1) Take a certain amount of basal medium stored at above 4°C, add agar powder at an amount of 30g agar powder per 1L, and then sterilize under high pressure at 121°C for 20 minutes;

[0097] 2) When the temperature of the culture medium drops to about 45°C-50°C, add the stock solution of various antibiotics according to the concentration of 9mg vancomycin, 3mg amphotericin, 6mg polymyxin and 6mg trimethoprim per 1L medium ;

[0098] 3) Add fresh sheep blood according to the amount of 10...

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Abstract

The invention involves a medium in the field of biological engineering.The medium is immersed from beef heart, cowbrobin immersion, cysteine, NA 2 HPO 4 , Elected protein, naCL, and agar powder.Make HP's cultivation sensitivity of 98.88 % and 100 % specific, which greatly improves the shortcomings of existing technology and improves the application of HP training methods.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a culture medium. Background technique [0002] Gastritis is inflammation of the gastric mucosa caused by various reasons and is one of the most common digestive system diseases. According to the priority of clinical onset, it can generally be divided into two types: acute and chronic gastritis; according to different etiologies, it can be divided into Helicobacter pylori-associated gastritis, stress gastritis, autoimmune gastritis, etc. The pathological and histological changes of gastritis caused by different etiologies are also different, usually including three processes, namely epithelial injury, mucosal inflammatory response, and epithelial regeneration. Acute gastritis can be divided into simple, erosive and hemorrhagic, corrosive, suppurative gastritis according to its pathological changes, and chronic gastritis can be divided into three categories: non-atrophic, atrophic a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12R1/01
CPCC12Q1/045G01N2333/195
Inventor 王洪涛张永顶马伟民
Owner SHENZHEN BLOT BIOTECH
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