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Streptomycete fermentation medium

A fermentation medium, Streptomyces technology, applied in the directions of fermentation, microorganism-based methods, microorganisms, etc., can solve the problem of high cost of Streptomyces fermentation medium, and achieve the advantages of increasing industrial added value, improving strain utilization, and improving fermentation efficiency. Effect

Inactive Publication Date: 2016-11-09
FUYANG HUAIBANG MACHINERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the invention is to solve the high cost defect of prior art Streptomyces fermentation medium, and provide a low-cost Streptomyces fermentation medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of streptomyces fermentation medium, it is prepared by the raw material of following percentage by weight:

[0022] Straw enzymatic hydrolyzate 7.3%, potato extract juice 6.8%, soybean meal hydrolyzate 5.5%, fish bone meal hydrolyzate 2.6%, wheat bran 2.3%, glucose 2.6%, dipotassium hydrogen phosphate 0.2%, magnesium sulfate 0.05%, sodium chloride 0.03%, the rest is water;

[0023] Its preparation process is as follows: adding various raw materials into water, adjusting the pH to 7.1-7.5, incubating at 121°C for 20 minutes to sterilize, and cooling to 32°C to obtain the product.

[0024] The straw hydrolyzate is prepared according to the following process:

[0025] Put the rice straw into the pulverizer for crushing to get the straw powder, then spread it into a flat layer with a thickness of 1cm, and then irradiate with ultraviolet light for 15min, and the ultraviolet intensity is 3000uW / cm 2 , then put it into the container, add twice the weight of water to s...

Embodiment 2

[0036] A kind of streptomyces fermentation medium, it is prepared by the raw material of following percentage by weight:

[0037] Straw enzymatic hydrolyzate 9.2%, potato extract juice 4.2%, soybean meal hydrolyzate 3.3%, fish bone meal hydrolyzate 4.7%, wheat bran 2.3%, glucose 1.5%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.05%, sodium chloride 0.03%, the rest is water;

[0038] Its preparation process is as follows: adding various raw materials into water, adjusting the pH to 7.1-7.5, incubating at 121°C for 20 minutes to sterilize, and cooling to 32°C to obtain the product.

[0039] The straw hydrolyzate is prepared according to the following process:

[0040] Put the rice straw into the pulverizer for crushing to get the straw powder, then spread it into a flat layer with a thickness of 1cm, and then irradiate with ultraviolet light for 15min, and the ultraviolet intensity is 3000uW / cm 2 , then put it into the container, add twice the weight of water to s...

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PUM

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Abstract

The invention belongs to the technical field of microculture, and discloses a streptomycete fermentation medium. The streptomycete fermentation medium is prepared from, by weight, 7.3-9.2% of straw zymolyte, 4.2-6.8% of potato diffusion juice, 3.3-5.5% of bean pulp hydrolysate, 2.6-4.7% of fishbone meal hydrolysate, 2.3-3.7% of wheat bran, 1.5-2.6% of glucose, 0.1-0.2% of dipotassium phosphate, 0.05-0.08% of magnesium sulfate, 0.02-0.03% of sodium chloride and the balance water. The streptomycete fermentation medium is low in cost and increases enterprise profits.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and in particular relates to a streptomyces fermentation medium. Background technique [0002] Streptomyces (streptomyces), its basal hyphae are multi-branched, generally the septum is sparse, rarely broken, and often produces various water-soluble or fat-soluble pigments; the aerial hyphae are slightly thicker than the basal mycelium, and are outer Enclosed by the sheath, the aerial hyphae are partially differentiated into straight, flexible, hook-and-loop to loose or tightly spiral spore filaments, often containing about 50 spores, and the short ones contain 5-10 spores; the spores are Arthrospores are formed by broken hyphae, some of which take off the outer sheath, and some carry the outer sheath or residue, the outer sheath determines the surface structure of the spore; the mole content of G+C in DNA is 69-76%. This genus has the largest number of species, and it is diffic...

Claims

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Application Information

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IPC IPC(8): C12P19/54C12R1/545
CPCC12P19/54
Inventor 施国洪
Owner FUYANG HUAIBANG MACHINERY
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