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Culture method and application of Staphylococcus sciuri F-E8-1

The technology of a staphylococcus squirrel and a culturing method is applied in the culturing field of staphylococcus squirrel, and achieves the effects of strong industrial adaptability, low operation cost and short time consumption

Active Publication Date: 2020-10-02
SHANDONG ZHUORAN BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] About the culture method of Staphylococcus squirrel strains, and its ability to act on wastewater, especially high-salt fermentation wastewater, and to effectively treat wastewater, there are few publications at present
[0005] As for the high-salt fermentation waste water, CN109136095A discloses a waste water produced by fermentation of monosodium glutamate, using three kinds of microorganisms, which can effectively use the waste water to prepare microbial protein and purify the waste water at the same time, but whether these three kinds of microorganisms have high salt tolerance and Whether it also has a good ability to purify wastewater in a high-salt environment, the above-mentioned patent does not involve

Method used

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  • Culture method and application of Staphylococcus sciuri F-E8-1
  • Culture method and application of Staphylococcus sciuri F-E8-1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1A

[0031] Staphylococcus squirrel ( Staphylococcus sciuri) F-E8-1, its culture method is as follows:

[0032] Staphylococcus squirrel (Staphylococcus sciuri) The F-E8-1 single colony was inoculated in the seed medium, cultured at 40°C for 15h, then inserted into the fermentation medium according to the inoculum amount of 5%, and cultivated at 40°C for 110h, the residual sugar in the wastewater can be reduced to About 0.25%.

[0033] The seed medium is composed of the following raw materials in weight percentage: yeast powder 0.45%, peptone 0.9%, sodium chloride 0.55%;

[0034] Staphylococcus squirrel (Staphylococcus sciuri) The fermentation medium of F-E8-1 is composed of the following raw materials by weight percentage: high-concentration glutamic acid waste liquid containing 25%wt ammonium sulfate, the rest is sterile water, and the pH value is adjusted to about 7.0 with 1M NaOH.

Embodiment 1B

[0036] Staphylococcus squirrel ( Staphylococcus sciuri) F-E8-1, its culture method is as follows:

[0037] Staphylococcus squirrel (Staphylococcus sciuri) The F-E8-1 single colony was inoculated in the seed medium, cultured at 37°C for 18 hours, then inserted into the fermentation medium according to the inoculation amount of 6%, and cultivated at 37°C for 120 hours, the residual sugar in the wastewater can be reduced to About 0.28%.

[0038] The seed medium is composed of the following raw materials in weight percentage: yeast powder 0.35%, peptone 1.2%, sodium chloride 0.35%;

[0039] Staphylococcus squirrel (Staphylococcus sciuri) The fermentation medium of F-E8-1 is composed of the following raw materials by weight percentage: high-concentration glutamic acid waste liquid containing 20% ​​ammonium sulfate, the rest is sterile water, and the pH value is adjusted to about 7.0 with 1M NaOH.

Embodiment 1C

[0041] Staphylococcus squirrel ( Staphylococcus sciuri) F-E8-1, its culture method is as follows:

[0042] Staphylococcus squirrel (Staphylococcus sciuri) The F-E8-1 single colony was inoculated in the seed medium, cultured at 42°C for 12 hours, then inserted into the fermentation medium according to the inoculation amount of 4%, and cultivated at 42°C for 96 hours, the residual sugar in the wastewater can be reduced to About 0.3%.

[0043] The seed medium is composed of the following raw materials in weight percentage: yeast powder 0.65%, peptone 0.8%, sodium chloride 0.65%;

[0044] Staphylococcus squirrel (Staphylococcus sciuri) The fermentation medium of F-E8-1 is composed of the following raw materials by weight percentage: high-concentration glutamic acid waste liquid containing 30% ammonium sulfate, the rest is sterile water, and the pH value is adjusted to about 7.5 with 1M NaOH.

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Abstract

The invention belongs to the technical field of microbial culture, particularly relates to a culture method of Staphylococcus sciuri capable of resisting a high-salt environment, and further relates to application of the Staphylococcus sciuri in treatment of fermentation production wastewater. The application of the Staphylococcus sciuri F-E8-1 capable of resisting the high-salt environment in rapid degradation of residual sugar in the fermentation production wastewater of organic acid and / or amino acid in the high-salt environment is the range of key protection of the invention. The specificfermentation production wastewater is treated through a microbial strain, so that the Staphylococcus sciuri F-E8-1 has the significant advantages of being low in operation cost, low in consumption, high in efficiency, high in adaptability, relatively stable in operation, convenient and fast to operate and manage and the like. The Staphylococcus sciuri provided by the invention has excellent high-salt resistance, and is good in treatment effect in the treatment process of a wastewater body with high salt content. The high-salt wastewater is treated by adopting the strain disclosed by the invention, so that the consumed time is short and the treatment effect is good.

Description

technical field [0001] The invention belongs to the technical field of microbial culture, in particular to a method for cultivating Staphylococcus squirrel which can tolerate a high-salt environment, and also relates to the aforementioned Staphylococcus squirrel Staphylococcus sciuri Application of F-E8-1 in the treatment of fermentation production wastewater. Background technique [0002] Regarding Staphylococcus squirrel, CN103865859A discloses a strain of Staphylococcus squirrel, a vaccine and a preparation method thereof, wherein Staphylococcus squirrel is mainly used as an inactivated vaccine; [0003] The following disclosures have been made about Staphylococcus squirrel in journal literature: In the article "Study on the Bacteriostasis and Mechanism of Composite Biological Preservatives against Staphylococcus squirrel" by Lan Weiqing et al. Gram-positive dominant bacteria - Staphylococcus sciuri (Staphylococcus sciuri) was used as the test bacteria, and the compound ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/44C02F103/36
CPCC12N1/20C02F3/34C02F2103/36C12R2001/44C12N1/205
Inventor 赵晨岳秋林赵林
Owner SHANDONG ZHUORAN BIOTECHNOLOGY CO LTD
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