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Method and device for collecting microalgae through continuous culture and in-situ self-flocculation

A technology of self-flocculation and microalgae, which is applied to the device for continuous cultivation and in-situ self-flocculation harvesting chlorella, cultivation and harvesting of microalgae, continuous cultivation and in-situ self-flocculation harvesting of chlorella, can solve the problem. Difficulty in separation, low algal cell density, and high production costs, to achieve the effects of reducing environmental pollution, simple equipment structure, and reduced processing capacity

Inactive Publication Date: 2013-05-08
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some problems in these methods: when cultivating at low density, the power cost of the centrifugation method is relatively high; the conventional flocculation method not only needs to use flocculants, but also the harvested culture solution cannot be recycled, and the production cost is relatively high. High, and if chemical flocculants are used, a large amount of waste water will cause environmental pollution; when collecting microalgae by filtration, it is easy to cause blockage of the filter cloth; sieving method is only suitable for microalgae with large individuals
[0004] The main difficulties in large-scale harvesting of chlorella are: (1) The density of algae cells in the culture medium is generally low. To obtain a certain scale of algae, a large number of cultures must be cultivated. Therefore, the treatment capacity of the culture medium is very large
(2) The individuals of Chlorella cells are relatively small, generally only 3~8μm, so the general solid-liquid separation method is not suitable
(3) When the concentration of chlorella reaches a certain value, the viscosity is very high and it is difficult to separate

Method used

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  • Method and device for collecting microalgae through continuous culture and in-situ self-flocculation
  • Method and device for collecting microalgae through continuous culture and in-situ self-flocculation

Examples

Experimental program
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Effect test

Embodiment 1

[0035] 1. Microalgae continuous culture and in-situ self-flocculation harvesting device

[0036] Microalgae continuous culture and in-situ self-flocculation harvesting device (refer to figure 1 ), including a column reactor 1 with an inverted cone 2 at the bottom (the height of the cone is 10-40 cm) (the material of the reactor is colorless and transparent organic glass; the column is cylindrical, and the inner diameter of the column is 10-40 cm , the height of the column shaft is 50~200 cm); and an algae discharge valve 4 is provided at the bottom of the inverted cone. The lower part of the column reactor 1 is provided with an aeration device, and a certain proportion of CO is introduced into the column. 2 of sterile air to achieve air supply and CO 2 biological fixation. The ventilation device includes a gas distributor 12 arranged in the column reactor 1 and a ventilation pipeline 10 installed outside the column reactor, a filter membrane or a filter element is arranged ...

Embodiment 2

[0050] 1. Microalgae continuous culture and in-situ self-flocculation harvesting device: the same as in Example 1.

[0051] 2. Microalgae continuous culture and in-situ self-flocculation harvesting process:

[0052] (1) Chlorella pyrenoidosa ( Chlorella pyrenoidosa ) Preparation of seeds: same as in Example 1;

[0053] (2) The configuration of wastewater medium: take some urban domestic wastewater, let it settle for pretreatment, take the supernatant and filter it with a 60-mesh filter, and then add nutrients (g / L) according to the following concentration: KNO 3 1.0 g / L, NaH 2 PO 4 0.2 g / L, NaHCO 3 0.5 g / L, MgSO 4 ·7H 2 O 0.1 g / L, CaCl 2 2H 2 O 50 mg / L, NaCl 200 mg / L, FeCl 3 . 6H 2 O 20 mg / L. Adjust the pH of the culture medium to 7.2; sterilize at 121°C, 0.1 MPa for 20 min, and then cool down for use.

[0054] (3) Inoculation and cultivation: inject the above medium into a sterilized column reactor, insert the wet heavy algae cell concentration at 400 mg / L,...

Embodiment 3

[0058] 1. Microalgae continuous culture and in-situ self-flocculation harvesting device: the same as in Example 1.

[0059] 2. Microalgae continuous culture and in-situ self-flocculation harvesting process:

[0060] (1) Chlorella pyrenoidosa ( Chlorella pyrenoidosa ) Preparation of seeds: same as in Example 1;

[0061] (2) Configuration of wastewater medium: Take wastewater from a certain farm, let it settle for pretreatment, take the supernatant and filter it with a 60-mesh filter, add tap water to dilute it by 1:1 volume, and then add nutrients at the following concentration ( g / L): Urea 0.25 g / L, K 2 HPO 4 0.05 g / L, MgSO 4 ·7H 2 O 0.075 g / L, CaCl 2 2H 2 O 50 mg / L, NaCl 100 mg / L, FeCl 3 . 6H 2 O 5 mg / L. Adjust the pH of the medium to 7.5; use microfiltration to sterilize.

[0062] (3) Inoculation and cultivation: inject the above medium into the sterilized column reactor, insert the wet heavy algae cell concentration at 250 mg / L, manually control the light in...

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Abstract

The invention provides a method and a device for collecting microalgae through continuous culture and in-situ self-flocculation and belongs to the technical field of microbial culture. The method comprises the following steps of: culturing microalgae cells through a bubble stirring column type photobioreactor, separating the microalgae cells through an in-situ sedimentation method, and discharging concentrated microalgae slurry from the bottom of a cone at the lower end of the column; and directly supplementing in flocculated supernatant and continuously culturing the microalgae cells. The organic wastewater recycling, CO2 biological fixation and continuous microalgae culture and separation can be simultaneously realized, the cost of culturing microalgae in a large scale is reduced, and the culture and recovery efficiency is improved; the culture solution is recycled, so that the treatment quantity is greatly reduced; the microalgae culture and recovery process is simple and is convenient to operate; and moreover, the equipment is simple in structure, low in cost and wide in application range and has the potential of industrial popularization and application.

Description

technical field [0001] The invention belongs to the technical field of microbial cultivation, and relates to a method for cultivating and harvesting microalgae, in particular to a method for continuously cultivating and in-situ self-flocculation harvesting of chlorella; A device for harvesting chlorella from flocculation. Background technique [0002] Algae have outstanding characteristics such as wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, high protein and oil content, and environmental friendliness. Chlorella is a single-cell green algae, rich in protein, vitamins, minerals, dietary fiber, nucleic acid and chlorophyll, etc. It is an indispensable nutrient for maintaining and promoting human health. Chlorella has the functions of enhancing human immunity, preventing virus proliferation, inhibiting cancer cell proliferation, lowering serum cholesterol levels, and detoxifying. Chlorella is recogniz...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12M1/00C12R1/89
CPCC12M33/22C12M31/02C12M21/02C12M23/08C12M41/12C12M29/26C12M27/02C12M29/20
Inventor 孔维宝刘娜张继牛世全杨红曾家豫
Owner NORTHWEST NORMAL UNIVERSITY
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