High temperature methane bacteria inoculating culture method

A technology for domestication and cultivation of methanogens, which is applied in the field of microbial cultivation, can solve the problems of high energy consumption in engineering operation and low gas production efficiency, and achieves the effects of solving high energy consumption, improving gas production rate and simple steps.

Active Publication Date: 2016-06-01
GANSU DEFU BIOTECH
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  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a domestication and cultivation method of high-temperature methane bacteria, which can obtain high-temperature methane bacteria by using the step-by-step heating and temperature-raising method; the met

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  • High temperature methane bacteria inoculating culture method
  • High temperature methane bacteria inoculating culture method
  • High temperature methane bacteria inoculating culture method

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example 1

[0020] Example 1, according to content of the present invention, propose detailed operation steps below

[0021] (1) Add peptone, glucose, sodium acetate and acetic acid to the anaerobic sludge containing methanogens from the bottom of the river to adjust the pH to 7.2, and carry out anaerobic constant cultivation for 2 days at a temperature of 32°C; The weight ratio is: anaerobic sludge: peptone: glucose: sodium acetate = 3:1:0.5:0.5.

[0022] (2) Add basal medium according to 1 / 4 of the volume of the incubator, and the pH of the medium is 7.2; then pass nitrogen into the incubator to exhaust the air, heat the medium to 25°C, and inoculate according to 10% of the volume of the medium The cultured mixture containing methanogens in step (1) is stirred and mixed evenly with the basal medium;

[0023] The basal medium consists of: sludge, poultry manure and potato dregs are mixed according to the ratio of 500:1500:2000;

[0024] The sludge is black domesticated anaerobic sludge...

example 2

[0029] (1) Add peptone, glucose, and sodium acetate to the anaerobic sludge containing methanogens from the bottom of the river, add acetic acid to adjust the pH to 7.2, and carry out anaerobic constant cultivation at a temperature of 32°C for 4 days. Its weight ratio is: anaerobic sludge: peptone: glucose: sodium acetate=3: 1: 0.5: 0.5;

[0030] (2) According to 1 / 4 of the volume of the incubator, add sludge, poultry manure and potato dregs to mix the basic medium according to the ratio of 1000:500:500, and the pH of the medium is 7.2; then pass nitrogen into the incubator to remove the air , heating the culture medium to 25° C., according to 10% of the culture medium volume inoculation step (1) containing the mixture containing methanogens, and stirring and mixing with the base medium;

[0031] (3) Heat up the culture medium inoculated with methanogens step by step. First, heat the culture medium at 25°C and raise the temperature by 1°C for 1 day and cultivate it for 10 days...

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Abstract

The present invention discloses a high temperature methane bacteria inoculating culture method, and belongs to the technical field of microbial culture. The basic process of the method is as follows: a basal culture medium is warmed to 25 DEG C, inoculated with methane bacteria, warmed to 35 DEG C for constant temperature culture, then warmed to 42 DEG Cfor constant temperature culture, then warmed to 45 DEG C, and then warmed to 55 DEG C for constant temperature culture. According to the method, a four-stage heating and three-stage constant temperature culture method is used to obtain high-temperature inoculating cultured methane bacteria; and the high temperature methane bacteria inoculating culture method helps to improve the number and activity of high-temperature methane bacteria, is directly applied to high-temperature fermentation methane manufacturing process, reduces the fermentation step and fermentation time of the high-temperature fermentation, and solves the problems of high energy consumption and low gas producing efficiency of high-temperature fermentation engineering in the prior art.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a method for acclimating and cultivating high-temperature methane bacteria. Background technique [0002] The biogas fermentation process is a complex biochemical process that is combined and alternately acted by a variety of microorganisms. During the fermentation process, various microorganisms carry out a series of coupled biochemical reactions, and the non-methanogenic bacteria and the methanogenic bacteria are interdependent and restrict each other, and are in a state of equilibrium during the fermentation process. Generally speaking, microbial growth conditions cannot change suddenly. Generally speaking, there must be an adaptation process. The domestication process should be as consistent as possible with the original growth conditions. The gas volume must create a suitable environment for the biogas fermentation microorganisms in terms of temperature, r...

Claims

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Application Information

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IPC IPC(8): C12N1/36
Inventor 卢新社
Owner GANSU DEFU BIOTECH
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