172 results about "Methane bacteria" patented technology

The invention relates to a treating method for energy regeneration, resource recovery and pollution free of restaurant garbage, which comprises the steps that separated liquid material is pumped into a swage concentrating pool; then the concentrated liquid material is discharged into a regulating and pretreating pool, in which methane bacteria are inoculated; the pretreated liquid material is discharged into an anaerobic fermentation pool, in which anaerobic bacteria groups are added so that methane gas is generated and the overflowing liquid material from the anaerobic fermentation pool is led to enter an aerobic aerated pool for aerobic reaction; the liquid material after the aerobic reaction is discharged into a sedimentation tank for sedimentation. In the comprehensive utilization of 'swill', the disposing method of the invention carries out anaerobic fermentation and aerobic aerated biochemical treatment to transform part of the energy in the swill into bio-energy which is then transformed into heat energy and finally into electrical energy that is connected with power grid for use. The separated solid material is smashed, dried, sterilized, dehydrated and then added with Chinese herbal medicine to be processed into Chinese herbal medicine compound feeds and fertilizers; meanwhile, the treatment of energy regeneration, resource recovery and pollution free of restaurant garbage for restaurant garbage is realized.

An anaerobic digester and a method for treating organic waste and recovering a usable quality methane gas. A first cover of gas permeable material conducive to bacterial colonization covers the surface of a slurry of organic waste material in a containment vessel. The permeable cover acts as a media to support and encourage the growth of methanogenic bacteria. Gas collection apparatus is installed on the first cover. A second cover of gas impermeable material is installed over the gas collection apparatus. A gas collection space is formed between the two covers. Edges of the first and second covers are closed to inhibit the escape of gas from the gas collection space. Biogas produced as a result of anaerobic digestion activity permeates the gas permeable cover and enters the collection space. From the collection space the gas is drawn into the gas collection apparatus which can, for example, be comprised of a network of perforated ducts connected to a blower that draws gas out of the ducts for delivery to a storage or usage location.

The invention discloses a method for treating wastewater in an artificial wetland and a wastewater treating device for implementing the method, wherein the method for treating the wastewater comprisesthe following steps: firstly, taking advantage of plant roots to absorb N, C and P from the wastewater so that part of N, COD, BOD and P can be removed; then utilizing nitrobacterium, aerobes and polyphosphate accumulationg bacteria to remove ammonia, organisms and phosphorus from the wastewater; then, utilizing acidified hydrolytic bacteria and anoxic bacteria to remove macromolecular organisms;and finally utilizing methane bacteria and anaerobes to remove organic acid and micromolecular organisms. In addition, the wastewater treating device comprises an artificial pond. The bottom of the artificial pond is provided with a bearing layer. An active carbon filter layer for filtering and purifying water is arranged above the bearing layer. A bacteria layer for eliminating and decomposing various pollutants in the wastewater is arranged above the active carbon filter layer. A layer of wetland filler is arranged between the active carbon filter layer and the bacteria layer. In the artificial pond, the bottom of the bearing layer is provided with a water draining pipe.

The invention relates to a methane-producing composite microbial inoculum and a preparation method thereof, which can solve the long-standing difficult problems that the existing biogas digester is lack of high-efficient methane-producing bacteria, thereby resulting in long initial start-up time of biogas production and poor stability of the biogas production. Methanosarcina acetoacidophilum DSM-N0.2834, methanobacterium formicicum DSM-No.1535, methanobrevibacter arboriphilicus DSM-No.1125, methanolobus tindarius DSM-No.2278 and methanothrix concilii DSM-No.6752 collected in German collection of microorganisms and cell cultures are adopted and inoculated in a liquid methane bacterial culture medium under the anaerobic condition, the independent enlarged culture is carried out grade by grade respectively, and bacterial liquid after the cultured methane bacteria is mixed together according to the volume ratio, thereby preparing a liquid methane-producing composite microbial inoculum; and the liquid methane-producing composite microbial inoculum can also be prepared into a solid methane-producing composite microbial inoculum. The method has reasonable design and adopts the high-efficient strains with the optimal combination and the rapid propagation technology, and the developed high-efficient methane-producing composite microbial inoculum can significantly accelerate the start-up time of the biogas production when in new construction of the biogas digester and large material change, and improve the efficiency and the stability of the biogas production of the biogas digester.

The invention discloses three-stage methane preparation device and method by utilizing surplus sludge, relating to methane preparation device and method and solving the environment pollution problem as most sludge is not duly handled currently. The three-stage methane preparation device by utilizing the surplus sludge mainly comprises an electrochemical oxidation tank, a hydrolytic acidification tank and a methane tank; and the three-stage methane preparation method by utilizing the surplus sludge is implemented based on the devices and sequentially comprises three stages of: a sludge electrochemical lysis hydrolysis stage, a sludge anaerobic acidification stage and a sludge anaerobic fermentation methane preparation stage, wherein the sludge electrochemical lysis hydrolysis stage comprises the steps of: destroying activated sludge floc, dissolving microbial cells and releasing macromolecular organic matters in the microbes by adopting an electrochemical oxidation technology so as to realize the sludge hydrolysis; the sludge anaerobic acidification stage comprises the steps of: controlling biological bacteria group to take acid-forming bacteria as principle matters, fermenting and acidifying hydrolyzed sludge and entering into the next stage when the pressure is greater than 1atm; and the sludge anaerobic fermentation methane preparation stage comprises the steps of: preparing methane by adopting up-flow anaerobic fermentation, selecting superior methane bacteria colonies by controlling the pH value and realizing the methane preparation. The invention can be used in the field of methane preparation by utilizing sludge.

The invention discloses a method for producing biogas by enhancing kitchen waste anaerobic fermentation, belonging to the technical fields of organic matter solid waste treatment and biomass new energies. The method comprises the following steps: (1) taking kitchen waste; (2) taking inocula; (3) mixing the kitchen waste and inocula, stirring uniformly, and adding the mixture into a fermentation reactor, wherein the solid concentration is 1.5-4%; (4) taking a ferrous salt, and preparing a 1.00-6.36 g/L solution; (5) adding the ferrous solution prepared in the step (4) into the fermentation reactor in the step (3) according to the addition amount of 1-250 mg/L; and (6) putting the fermentation reactor in the step (5) under moderate temperature conditions, fermenting, and collecting the biogas. The ferrous ions with proper concentration are added into the kitchen waste fermentation system, thereby effectively enhancing the activity of methane bacteria, increasing the organic matter degradation rate, increasing the biogas production rate, shortening the fermentation time and enhancing the economic benefit.

The invention relates to a warming device for a methane tank based on a complementary application of wind power and solar photovoltaic generation, belonging to the technical field of new energy application. A solar battery produces direct current under the sun, the direct current is input to a photovoltaic controller and adjusted, the direct current is then input to a photovoltaic inverter and converted to alternating current, the alternating current is input to a photovoltaic warming device, electric energy is then converted to heat energy, and the heat energy emits in a fermentation liquid; and wind blows blades of a wind-driven generator to rotate around a shaft of the wind-driven generator and drives the wind-driven generator to operate to produce direct current, the direct current is input to a wind powder controller and adjusted, the direct current is then input to a wind powder inverter and converted to alternating current, the alternating current is input to a wind powder warming device, and heat energy also emits in a fermentation liquid. The new wind and photovoltaic energy obviously raises the temperature of the fermentation liquid in a sweating room in the methane tank, promotes the growth and the propagation of methane bacteria dispersed in methane raw materials, and accelerates the organism decomposition speed of the methane bacteria in an appropriate temperature environment, thereby increasing the yield of methane.

The invention discloses a process for preparing fresh scent strong-flavor Chinese spirit. The process comprises distiller's yeast preparation, bacterial sludge preparation, construction of bacterial sludge pits and fermenting and distilling, wherein the step of bacterial sludge preparation comprises material pretreatment, culture of methane bacteria liquid and pit sludge fermentation and culture. Sludge pits in which methane bacteria serve as a primary flora and caproic acid bacteria serve as a secondary flora are constructed by adopting common conditions such as tile crocks and pits in common liquor factories, and conditions are created for producing fresh scent strong-flavor Chinese spirits. According to the process, the technical problem of factory-like methane bacteria anaerobic culture is overcome, and preparation of the sludge pits in which methane bacteria serve as the primary flora and caproic acid bacteria serve as the secondary flora is realized. The liquor is brewed by utilizing the prepared sludge pits and process thereof, and a fresh scent strong-flavor Chinese spirit with a primary component of ethyl acetate, a secondary component of ethyl caproate and an auxiliary material of 2,3-butanedione is formed. Partial characteristics of traditional daqu liquor and Xiaoqu wine processes are combined, and a process of the fresh scent strong-flavor Chinese spirit is formed.

The invention relates to a sludge micro oxygen hydrolytic acidizing method, which includes: inoculating sludge containing acid-producing bacteria in a hydrolytic acidizing reactor, intermittently inputting air to the middle of the hydrolytic acidizing reactor for air stirring, simultaneously supplementing intermittent mechanical stirring, performing hydrolytic acidizing on sludge of a sewage treatment plant under micro oxygen conditions with dissolved oxygen (DO) kept to be 0.4-0.8 mg/L, leading the sludge after hydrolytic acidizing treatment to enter a settling pond for sludge-water separation, and leading liquid supernatant to serve as carbon sources for municipal sewage biological de-nitrification and dephosphorization treatment. The sludge micro oxygen hydrolytic acidizing method is conducted at normal temperature, is not needed to be added with acid and alkali for adjusting potential of hydrogen (pH) value, is simple in operation, stable in running and capable of restraining growth of methane bacteria and improving stability of a sludge hydrolytic acidizing system, and simultaneously leads sludge to obtain reduction and stabilization treatment to the maximum extent. Sludge acid yield is improved by 38%-50%.

The invention discloses a man-made ebony production method and relates to a wood modifying technology. The method comprises the steps of carrying out first-stage fermentation, namely arranging special wood in lactic acid bacteria-yeast fermentation liquor so as to completely soften wood fiber and communicate intervals of cell walls; carrying out second-stage fermentation, namely after methane bacteria generated by fermentation enters the inner part of wood, carrying out anaerobic fermentation on biogas gas to generate methane gas; heating and pressurizing for catalysis, namely generating gas phase carbon by methane gas residual at the inner part of wood, and depositing the gas phase carbon on the wood fiber so as to obtain the man-made ebony. According to the method, most part of wood fiber is carbonized to be modified; the color and luster of the processed wood are similar to the color and luster of natural ebony; the index of the man-made ebony is similar to the materialization index of the natural ebony; the method is suitable for the modification of wood such as phoeba bournei, toona ciliata roem., camphorwood and pterocarya stenoptera.

A process for producing methane from process water and biogenic material such as that occurring in the production of sugar and ethanol, wherein at least one mixing/preliminary tank is supplied with process water and biomass, and optionally with washing and/or fresh water and/or substrate water to produce a mash, the mash is set to a suitable pH value and temperature. The mash is transferred into at least one bioreactor with anaerobic methane bacteria for biogas production, the biogas developed is extracted and the biodegraded fluid is drawn off. A biogas plant for performing this process has at least one mixing or preliminary tank for mash provided with a heat exchanger system, and at least one downstream biogas reactor having bacteria producing biogas. The biogas plant is used for producing biogas and storing waste and process water and by-products from the sugar and ethanol production operation.

The invention relates to a method which utilizes organisms of straws, chicken manure, cattle manure, pig manure, sheep manure and horse manure to mix and agitate with zymogeneous bacteria. The method comprises the following steps: firstly filling the organisms in a ground pool, covering the upper part of the organisms for 3 to 5 days by using a plastic film; then refilling the organisms in a marsh gas generator after the temperature is up to 60 DEG C; adding 1 to 2 percent of limewater, so as to neutralize organic acid generated; controlling the pH value to be within 6.8 to 7.5, so that acid-producing bacteria and methane bacteria are subjected to the dry fermentation of anaerobic exudate and marsh gas; after the speed is balanced, generating anaerobic bacteria, and generating mixed gas; and generating pure march gas after the mixed gas is desulfurized, dehydrated and purified. The gas production amount of the organisms per kilogram averagely reach 0.35m<3>, the gas production rate averagely is 0.398m<3>. The method is widely applied to cooking and illuminating in a family.

The invention discloses a methane tank capable of facilitating slag removal. The methane tank comprises a methane tank body, wherein a feed port, a discharge port and a methane outlet are formed on the methane tank body; a gas storage chamber is arranged on the upper part in the methane tank body; a fermenting chamber is arranged on the lower part in the methane tank body; the bottom surface of the methane tank body is inclined; the feed port corresponds to the higher part of the inclined surface; and the discharge port corresponds to the lower part of the inclined surface. The bottom surface of the methane tank body is set to be the inclined surface, so when removing methane slag, a user only needs to draw out one part of methane liquid and return the methane liquid from the feed port (a feed pipe) to impact the bottom surface of the methane tank body to push the methane slag into the discharge port (a water pressure room) along with the inclined surface, and then can take out the methane slag conveniently from the discharge port (the water pressure room), so the user does not need to enter the fermenting chamber to remove the slag under a poisoning risk, and time and labor are saved; and in addition, the tank is stirred by the impact of the methane liquid, so that methane bacteria completely contact with fermenting materials newly entering the tank and yield of the methane is increased.

The invention discloses a culture method and culture medium of a cellar mud bacterium liquid with special flavor-producing function. The culture medium comprises the following components in percentage by weight: 3-5% of waste fermented grains, 8-10% of high-quality cellar mud, 10% of bottom fermented grain immersion liquid, 0.2-0.5% of K2HPO4, 0.1-0.3% of NaAC, 0.005-0.01% of MgSO4, 2-4% of 95% alcohol and 5-10% of barium sulfide solution. The cellar mud bacterium liquid with special flavor-producing function, which is cultured by the culture medium, can provide a hexanoic acid bacteria/methane bacteria/nitrate reducing bacteria compound flora which takes effect on producing compound fragrance of a wine body, and solves the problem of lack of compound fragrance of the wine produced in thepresent cellar which serves for a short time.

The invention discloses a mechanical pulping method for biological bacteria liquid, and belongs to the field of papermaking and pulping. The main technical problem to be solved is that the waste liquor for pulping pollutes the environment. The method is that: compound bacteria serve as bacterial strains; the compound bacteria comprise white rot fungi, actinomycete, and methane bacteria in a mass ratio of 1-3: 1-3: 1; and the density of the mixed bacterial liquid is 60,000,000/ml. The method comprises the following steps: degrading the raw materials with the mixed bacterial liquid after swelling, thread rolling and extrusion; grinding coarsely; grinding finely; bleaching or not bleaching; and washing the liquid to prepare the pulp. The method is mainly used for pulping herbal materials and has the advantages of no pollution or discharge of waste liquor in the pulping process, recyclable liquid, low production cost and simple process.

The invention provides a pit mud formula for brewing, pit mud and a making process thereof. The pit mud formula comprises, by weight, 40-60 parts of yellow mud, 15-25 parts of sapropel, 1-10 parts of yeast powder, 1-10 parts of parent grains, 5-15 parts of yeast wine and 5-15 parts of a culture solution. The making process of the pit mud for brewing includes the steps of material preparation according to the formula, preparation of a culture solution, pretreatment for crushing and mixing certain raw materials and fermentation. According to the making process of the pit mud, the required fermentation time is short, the making steps are simple, and operability is high; the pit mud made according to the pit mud formula contains abundant beneficial fermentation microorganisms, and caproic acid bacteria, methane bacteria and butylic acid bacteria are rich in content and high in reproductive ability. The organoleptic quality and all physical and chemical parameters of the pit mud are not significantly different from those of pit mud which is 30 years old.

Provided is a method for performing anaerobic digestion by utilizing a heavy metal ion enriched biomass waste material, comprising: putting a biochar made from the raw materials such as garden rubbish and agricultural and forestry waste, or a plant for adsorbing heavy metal ions in waste water containing heavy metal ions, and obtaining a heavy metal saturated biochar or a heavy metal super-enriched plant; and then adding in a kitchen waste anaerobic digestion methane generation system at a certain ratio. The heavy metal ions adsorbed in the plant or the biochar are utilized as a basic component of an anaerobic reaction enzyme to promote the growth of methane bacteria, activate the activity of the enzyme, shorten the time of a hydrolysis acidification stage, reduce VFA accumulation, and accelerate the generation of energy products such as methane and the like.

The invention discloses a method of producing biogas through Chinese medicine residues, relating to application of Chinese medicine residues and belonging to the field of utilization of biomass energy sources. The method is characterized by comprising the following steps of: crushing the Chinese medicine residues to 40 meshes, filling methane bacteria accounting for 10% of total amount of the Chinese medicine residues, adjusting the carbon-nitrogen ratio to be (20-25):1, and treating at 53-55 DEG C for 10 days; and performing an anaerobic digestion reaction to prepare biogas. The method has the beneficial effects that biogas preparation and combustion equipment is simple, the equipment is conveniently used and managed, rely of urban residents on fossil energy and emission of greenhouse gases are reduced, and the biogas contains no such poisonous gases as CO, thereby being more beneficial to human body health and excellent in environmental effect; the straw combustion quantity is reduced to create conditions for straw application and realizing sustainable utilization of rural lands; and the biogas is used for replacing the straws to greatly improve the living environment of farmers and improving the living standard of the farmers, thereby having excellent social benefit and ecological benefit.

The invention discloses a system and method for generating power by using biomass. The system comprises a biogas preparation subsystem, a methane preparation subsystem, a hydrogen preparation subsystem and a hydrogen power generation subsystem, wherein the biogas preparation subsystem is used for fermenting organics in the biomass serving as a raw material with methane bacteria to generate biogas under the condition of air isolation; the methane preparation subsystem is used for extracting methane from the generated biogas and oxidizing the methane to generate methanol; the hydrogen preparation subsystem is used for preparing hydrogen in a way of mixing generated methanol and water or directly preparing hydrogen by using the generated methanol; and the hydrogen power generation subsystem is used for generating power by using the generated hydrogen. According to the system and the method for generating power by using the biomass, provided by the invention, the biogas is prepared from the biomass, the methane is extracted from the biogas, the methane is oxidized to prepare the methanol, the hydrogen is prepared from the methanol, and the hydrogen is used for generating power, thus the biomass is converted into electric energy and the energy conversion efficiency is increased.

The invention relates to a coastal sandy soil saline land soil modifier and applications of the coastal sandy soil saline land soil modifier in sorghum-sudangrass hybrid planting. The present invention discloses a coastal sandy soil saline land soil modifier, which comprises, by mass, 30-35 parts of organic fertilizer cow manure, 8-10 parts of potassium feldspar, 3-5 parts of hydrolyzed polyacrylonitrile, 1-3 parts of asparagus officinalis residue, 3-5 parts of boric acid, 7-10 parts of sawdust, 12-15 parts of fulvic acid, 6-8 parts of biochar, 5-7 parts of polyvinyl alcohol, 3-5 parts of polymeric amino acid, 0.6-0.8 part of a soil thickener, 3-8 parts of gaillardia pulchella foug powder, 7-9 parts of Yeqiexiang, 0.2-0.8 part of a water retaining agent, 0.05-0.07 part of Bacillus coagulans, and 0.2-0.6 part of halophilic methane bacteria. According to the present invention, 12 months after the sorghum-sudangrass hybrid is planted, the pH value of the soil is 7.0-7.3, the soil alkalinity is 1.8-2.5%, the soil salt content is 0.11-0.18%, and the soil water holding capacity is 42-47%.

Provided is a composite material for reducing greenhouse gases discharge and application thereof. The composite material is made by mixing raw material being rich of sulphates and ferric iron oxide or hydroxides, the molar ratio of S and Fe in the composite material is from 20:1 to 1:3, the composite material is a particle or powder solid matter. By means of adding the composite material particle or powder into a circumstance being rich of organic matter and anaerobic microorganism such as a pool, a cityscape water, a river, a ditch, a paddy field, an artificial wetland, a cienaga, a lake, a sea and a refuse landfill, it is capable of restraining the activity of methane bacteria, translating unstable organic carbon into inorganic carbonate and reducing greenhouse gases discharge with the action of the composite material and the microbe.

The invention discloses a device and a method for producing methane by sludge removal filtrate. The device comprises an anaerobic reactor, wherein the anaerobic reactor is divided into a sludge reaction area, a three-phase separation area and a gas chamber from top to bottom; the sludge reaction area is provided with a conductive carrier layer; a conductive carrier in the conductive carrier layer consists of carbon fibres and carbon black at the mass ratio of (1:1)-(2:1). The carbon fibres and the carbon black are arranged in the anaerobic reactor to serve as the conductive carrier layer, so that electrons are transferred between methanogenic archaea and syntrophism bacteria through electron carriers of hydrogen, methanoic acid or the like, and inter-specific electron transfer can also be performed directly; therefore, more methane producing ways are provided for producing methane bacteria; the conductive carrier layer is fixed to the anaerobic reactor so as not to lose easily along with the outflow of water flow, and the conductive carrier layer can also be in full contact with wastewater so as to improve the methane producing rate; the water penetrating load of the conductive carrier layer is increased by adopting wastewater cycling, and meanwhile, a fluidizing effect of sludge is also improved; therefore, the methane producing efficiency is improved.

The invention belongs to the technical field of paper-making wastewater treatment, and in particular provides a paper-making wastewater resource recycling and treating process method and a paper-making wastewater resource recycling and treating system device. After the method and the device are used, a better paper-making wastewater treatment effect is achieved by means of ultrasonic pretreatment,the chemical oxygen demand (COD) removal rate can be increased by about 20%, and the total COD removal rate can reach 57-69%; after black liquid is treated by means of ultrasonic waves, the comprehensive toxicity is reduced, the sludge activity is enhanced, the active period shifts forward, and bone morphogenetic protein (BMP) of at the active period of methane bacteria is obviously improved.