Methane-producing composite microbial inoculum and preparation method thereof

Abstract

The invention relates to a methane-producing composite microbial inoculum and a preparation method thereof, which can solve the long-standing difficult problems that the existing biogas digester is lack of high-efficient methane-producing bacteria, thereby resulting in long initial start-up time of biogas production and poor stability of the biogas production. Methanosarcina acetoacidophilum DSM-N0.2834, methanobacterium formicicum DSM-No.1535, methanobrevibacter arboriphilicus DSM-No.1125, methanolobus tindarius DSM-No.2278 and methanothrix concilii DSM-No.6752 collected in German collection of microorganisms and cell cultures are adopted and inoculated in a liquid methane bacterial culture medium under the anaerobic condition, the independent enlarged culture is carried out grade by grade respectively, and bacterial liquid after the cultured methane bacteria is mixed together according to the volume ratio, thereby preparing a liquid methane-producing composite microbial inoculum; and the liquid methane-producing composite microbial inoculum can also be prepared into a solid methane-producing composite microbial inoculum. The method has reasonable design and adopts the high-efficient strains with the optimal combination and the rapid propagation technology, and the developed high-efficient methane-producing composite microbial inoculum can significantly accelerate the start-up time of the biogas production when in new construction of the biogas digester and large material change, and improve the efficiency and the stability of the biogas production of the biogas digester.

Description

technical field [0001] The invention relates to a methanogenic composite bacterial agent and a preparation method thereof. The methanogenic composite bacterial agent is mainly used as a start-up agent for newly built biogas digesters and biogas digesters lacking methanogenic bacteria, which can speed up the start-up of biogas production in newly built biogas digesters, and improve the biogas production rate. capacity. Background technique [0002] At present, according to relevant literature reports, the inoculum for starting biogas fermentation is mainly the fermentation broth of old biogas tanks, sewage sludge, pond sludge, old cesspit sludge, etc., which contain a large number of methanogenic bacteria. In the process of vigorously promoting biogas, one of the most prominent and pervasive problems is that the initial start-up time of biogas production is long, especially in places where new biogas digesters are newly built, especially in the north, it is difficult to find ...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a methanogenic composite bacterial agent and its preparation method, which solves the long-standing problems of lack of efficient methanogenic bacteria in existing biogas digesters, resulting in long initial start-up time of biogas production and poor gas production stability. The design of the method is reasonable, ...