Rhodopseudomonas palustris culture medium and application thereof

A technology of Rhodopseudomonas swamp, Rhodopseudomonas, applied in bacteria, microorganism-based methods, and adding compounds to stimulate growth, etc., can solve the problem of limiting the application of photosynthetic bacteria, low effective viable bacteria, and poor product stability. and other problems, to achieve the effect of promoting the growth and reproduction of bacteria, high effective number of viable bacteria, and stable quality

Active Publication Date: 2020-06-05
CHAMBROAD CHEM IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the limited literature on in-depth research on the high-density fermentation formula of photosynthetic bacteria, the existing commercially available products generally have low ef

Method used

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  • Rhodopseudomonas palustris culture medium and application thereof
  • Rhodopseudomonas palustris culture medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A culture medium for Rhodopseudomonas palustris, formula: citric acid 1.05g / L, beef extract 2.75g / L, yeast extract 0.52g / L, peptone 1.93g / L, sodium acetate 0.50 / L, magnesium sulfate 0.35g / L, calcium chloride 0.25g / L, potassium dihydrogen phosphate 0.14g / L, ferrous sulfate 5mg / L, VB 60.2mg / L, compound inorganic salt solution 0.5mL / L (EDTA 0.5g / L, zinc sulfate 0.01g / L, boric acid 0.03g / L, manganese chloride 0.003g / L, calcium chloride 0.02g / L, chlorine Copper chloride 0.001g / L, sodium molybdate 0.003g / L), pH 6.8-7.0; potassium dihydrogen phosphate, complex inorganic salt solution, and other remaining components in the medium were sterilized at 115°C for 10 minutes.

[0032] The above medium was used as the experimental group, and the control group was set up. The medium used the traditional RCVBN formula: DL-malic acid 4.0g / L, magnesium sulfate 0.12g / L, ammonium sulfate 1.0g / L, calcium chloride 0.075g / L , potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosph...

Embodiment 2

[0039] A kind of rhodopseudomonas palustris culture medium, preparation steps are as follows (taking 20L culture medium as example):

[0040] (1) Weigh 22.0g of citric acid, 57.0g of beef extract, 12.0g of yeast extract, 39.0g of peptone, 11.0g of sodium acetate, 8.0g of magnesium sulfate, 160.0mg of ferrous sulfate, VB 6 4.0mg, calcium chloride 5.0g in a beaker, adjust the pH to 6.8 with 20% potassium hydroxide, dilute the volume to 19.9L with pure water, stir to mix evenly;

[0041] (2) Weigh 4.0 g of potassium dihydrogen phosphate into a beaker, dilute it to 100 mL with pure water, and stir to mix evenly;

[0042] (3) Weigh 0.5g of EDTA, 0.01g of zinc sulfate, 0.03g of boric acid, 0.003g of manganese chloride, 0.02g of calcium chloride, 0.001g of copper chloride, and 0.003g of sodium molybdate in a beaker. 1000mL, stir to make it evenly mixed;

[0043] (5) Separate the three solutions in steps (1), (2) and (3) and sterilize at 115°C for 10 minutes.

[0044] (6) The ster...

Embodiment 3

[0053] A kind of rhodopseudomonas palustris culture medium, preparation steps are as follows (taking 20L culture medium as example):

[0054] (1) Weigh 18.0g citric acid, 30.0g beef extract, 10.0g yeast extract, 36.0g peptone, 10.0g sodium acetate, 6.0g magnesium sulfate, 100.0mg ferrous sulfate, VB 6 3.0mg, calcium chloride 4.0g in a beaker, adjust pH to 6.8 with 20% potassium hydroxide, dilute pure water to 19.9L, stir to mix evenly;

[0055] (2) Weigh 2.0 g of potassium dihydrogen phosphate in a beaker, dilute it to 100 mL with pure water, and stir to make it evenly mixed;

[0056] (3) Weigh 0.5g of EDTA, 0.01g of zinc sulfate, 0.03g of boric acid, 0.003g of manganese chloride, 0.02g of calcium chloride, 0.001g of copper chloride, and 0.003g of sodium molybdate in a beaker. 1000mL, stir to make it evenly mixed;

[0057] (4) Separate the three solutions in steps (1), (2) and (3) and sterilize at 115°C for 10 minutes.

[0058] (5) The sterilized solution is cooled to 60-8...

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Abstract

The invention belongs to the technical field of microbial culture, and specifically relates to a rhodopseudomonas palustris culture medium and an application thereof. The rhodopseudomonas palustris culture medium has a formula composed of citric acid, a beef extract, a yeast extract, peptone, sodium acetate, magnesium sulfate, ferrous sulfate, VB6, calcium chloride, monopotassium phosphate and a composite inorganic salt solution, wherein a solvent is water. According to the invention, through optimization of the formula, the quality of a rhodopseudomonas palustris inoculum is greatly improved,so the rhodopseudomonas palustris inoculum is high in effective viable count, pure in color, free of layering, low in infectious microbe rate, stable in quality and good in preservation effect, and the rhodopseudomonas palustris inoculum cultured by using the culture medium provided by the invention has good effect when being applied to aquaculture.

Description

technical field [0001] The invention belongs to the technical field of microorganism culture, and in particular relates to a culture medium for Rhodopseudomonas palustris and an application thereof. Background technique [0002] Rhodopseudomonas palustris belongs to Rhodospirillaceae and Rhodopseudomonas genus, and is the most widely studied and used photosynthetic bacteria. Photosynthetic bacteria are the earliest prokaryotic organisms that appeared on the earth, are ubiquitous in nature, and have a primitive photoenergy synthesis system. , ammonia and other microorganisms that perform photosynthesis as hydrogen donors and carbon sources. Photosynthetic bacteria are widely distributed in soil, paddy fields, swamps, lakes, rivers and seas in nature, and are mainly distributed in anoxic areas where light can penetrate in aquatic environments. Rhodopseudomonas palustris is one of the feed-grade microorganisms allowed by the Ministry of Agriculture of my country. Its preparat...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38C02F3/34C12R1/01C02F103/20
CPCC12N1/20C12N1/38C02F3/34C02F2103/20
Inventor 杨丹丹吴文雷徐泽平张心青杨传伦马娜娜张甲庆陈振发潘冬梅
Owner CHAMBROAD CHEM IND RES INST CO LTD
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