Multi-phase co-culture and detection method and device thereof

Abstract

The invention relates to a multi-phase co-culture and detection method and a device thereof, and belongs to the technical field of microbe culture. According to the multi-phase co-culture and detection device, a culture tank (1.1) and a culture chamber (1.2) with a plurality of small chambers are formed on a base plate; and liquid culture media are injected into the culture tank (1.1), and various solid or semisolid culture media are injected into the small chambers of the culture chamber (1.2). The liquid culture media are coated to the solid or semisolid culture media by shaking the device to complete the inoculated culture process of bacterial colonies and obtain corresponding detection results. By the device, the solid-phase culture media, the liquid-phase culture media and the semisolid-phase culture media can be separated with each other and can contact with each other under a certain physical condition, so that the complex operation of inoculating bacteria between different culture media is avoided, a false positive result caused by microbial pollution of environment is reduced, the culture and detection of microbial samples are simplified, the stability of detection efficiency and detection results is improved, and the culture and detection can also be performed on a large scale.

Description

technical field [0001] The invention relates to a microorganism cultivation and detection method and a device thereof, belonging to the technical field of microorganism cultivation. Background technique [0002] Microbiological detection is the most basic and important work in the fields of medical and health, inspection and quarantine, food safety, and disease control. How to quickly and accurately identify the biological species and genetic variation status of microorganisms contained in samples has become an indispensable goal in daily inspection work. [0003] Bacterial culture medium can be divided into enrichment medium, selective medium and differential medium according to its function. Currently commercially available culture media or culture devices have single functions, and cannot be compatible with the above three functions at the same time. The inspection process of bacteria is generally to use the enrichment medium or the selection medium respectively, that i...

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Problems solved by technology

In addition, the traditional method can only detect the number of bacterial colonies and determine the species of bacteria, and the conclusion can only be drawn by identifying the medium for biochemical reactions

[0007] In CN201695031, a kind of divided petri dish is introduced, which makes up for the deficiency that the above-mentioned traditional petri dish can only carry out single microorganism cultivation and detection, and realizes several microorganism cultivation and detection under the same condition simultaneously, but it still adopts marking Spreading inoculation, inoculation efficiency is low, not suitable for large-scale operation

[0008] In CN202139235, a biphasic culture bottle is introduced, which achieves effective contact between the solid phase medium and the liquid phase medium, but because the complete separation of the solid phase and the liquid phase cannot be achieved, the nutrient molecules may diffuse in the two phases , leading to the fallacy of the result

In addition, the limited size of the bottle mouth and the vertical surface of the solid medium make it inconvenient to take solid colonies for positive transformation

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