Hirsutella sinensis expansion culture medium and hirsutella sinensis cultured by same as well as culture method

A technology of Trichospora chinensis, culture method, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of many operation steps, difficult to mass-produce, and low effective components for kidney health care.

Active Publication Date: 2018-11-23
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the past 20 years, some domestic scientific research institutes have successively carried out research on artificial cultivation and submerged fermentation culture of Cordyceps mycelium, but most of the health care products of Cordyceps fungus stay in the stage of using liquid culture mycelium, using Mortierella sinensis species Bacteria powder is produced by fermentation method. The disadvantage is that there are many operation steps, it is not easy to produce on a large scale, and the active ingredients of kidney health care in the bacteria powder are relatively low.

Method used

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  • Hirsutella sinensis expansion culture medium and hirsutella sinensis cultured by same as well as culture method
  • Hirsutella sinensis expansion culture medium and hirsutella sinensis cultured by same as well as culture method

Examples

Experimental program
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Effect test

Embodiment 1

[0041] (1) Strain activation: weigh 200g potato flour, 20g glucose, 10g peptone, 5g yeast powder, 0.5g KH 2 PO 4 , 0.5g MgSO 4 10g of agar, dissolved in water to 1L, stirred evenly, sterilized at 121°C for 20 minutes, placed on a slant or a flat plate to prepare an improved solid PDA medium. Inoculate the Mortierella sinensis species, cultivate at 17°C for 52 days, and obtain the activated Mortierella species for use.

[0042] (2) Propagation of bacterial strains: mix 6g silkworm chrysalis powder with 12ml water, boil for 20mim to extract, filter to obtain silkworm chrysalis extract and silkworm chrysalis residue respectively. Take silkworm chrysalis extract, weigh 20g of glucose, 10g of peptone, 5g of yeast powder and 0.1g of vitamin B1, mix them, add water to dissolve, and dilute to 1L to obtain an expansion medium. Divide the expansion medium into 250ml Erlenmeyer flasks, 150ml per bottle, and sterilize at 121°C for 20min. The activated Mortierella sinensis strain was i...

Embodiment 2

[0046] (1) Strain activation: weigh 220g potato flour, 18g glucose, 9g peptone, 4g yeast powder, 0.5g KH 2 PO 4 , 0.3gMgSO 4 , 9g of agar, dissolved in water to 1L, stirred evenly, sterilized at 121°C for 20 minutes, placed on a slant or a flat plate to prepare an improved solid PDA medium. Inoculate the Mortierella sinensis species, cultivate at 16°C for 45 days, and obtain the activated Mortierella species for use.

[0047] (2) Propagation of bacterial strains: mix 6g of silkworm chrysalis powder with 13ml of water, boil for 18mim to extract, filter to obtain silkworm chrysalis extract and silkworm chrysalis residue respectively. Take silkworm chrysalis extract, weigh 21g of glucose, 11g of peptone, 7g of yeast powder and 0.06g of vitamin B1, mix them, add water to dissolve, and dilute to 1L to obtain an expansion medium. Divide the expansion medium into 250ml Erlenmeyer flasks, 150ml per bottle, and sterilize at 121°C for 20min. The activated Mortierella sinensis strain...

Embodiment 3

[0051] (1) Strain activation: Weigh 180g potato flour, 23g glucose, 11g peptone, 6g yeast powder, 0.4g KH 2 PO 4 , 0.5g MgSO 4 11 g of agar, dissolved in water to 1 L, stirred evenly, sterilized at 121°C for 20 minutes, placed on an inclined plane or a flat plate to obtain an improved solid PDA medium. Inoculate the Mortierella sinensis species, cultivate at 18°C ​​for 60 days, and obtain the activated Mortierella species for use.

[0052] (2) Propagation of strains: mix 5g of silkworm chrysalis powder with 11ml of water, boil for 25mim to extract, filter to obtain silkworm chrysalis extract and silkworm chrysalis residue respectively. Take silkworm chrysalis extract, weigh 19g glucose, 9g peptone, 4g yeast powder and 0.15g vitamin B1, mix them, add water to dissolve, and dilute to 1L to obtain expansion medium. Divide the expansion medium into 250ml Erlenmeyer flasks, 150ml per bottle, and sterilize at 121°C for 20min. The activated Mortierella sinensis strain was inocula...

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Abstract

The invention provides a hirsutella sinensis expansion culture medium and hirsutella sinensis cultured by same as well as a culture method, belonging to the technical field of microorganism culture. Every 1L of the expansion culture medium is prepared from the following components: 8-12g of silkworm chrysalis extract, 15-25g of glucose, 8-12g of peptone, 3-8g of yeast powder and 0.05-0.2g of vitamin B1, and water is used for fixing the volume to 1L. Compared with the natural cordyceps sinensis, the hirsutella sinensis cultured by using the expansion culture medium provided by the invention islittle in differences, so that the hirsutella sinensis has the potential to become a substitute for the cordyceps sinensis.

Description

technical field [0001] The invention belongs to the technical field of microorganism culture, and in particular relates to a medium for expanding Mortierella chinensis, cultured Mortierella chinensis and a culture method thereof. Background technique [0002] Cordyceps sinensis (Cordyceps sinensis) is a widely accepted medicinal fungus, which is mass-proliferated in vivo after the larvae of Hepialus armoramus Oberthur of the genus Lepidoptera are infected with the fungus Hirsutella sinensis And the formation of insect-bacteria complex. Cordyceps sinensis is a traditional traditional Chinese medicine in my country. It is as famous as ginseng and antler. It has the functions of inhibiting the growth of tumor cells, regulating immunity, promoting hematopoiesis, anti-inflammatory and antibacterial, anti-arrhythmia, and anti-virus. [0003] In the past 20 years, some domestic scientific research institutes have successively carried out research on artificial cultivation and subm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 沙涛张亚平何志红杨小雨王丁卉
Owner YUNNAN UNIV
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