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Submerged fermentation culture medium of bacillus mucilaginosus applied to microbial fertilizers

A technology of Bacillus colloid and microbial fertilizer, applied in the field of microbial culture, can solve the problem of high cost of raw materials, and achieve the effects of simplified operation, low nutrient concentration and reasonable carbon-nitrogen ratio

Active Publication Date: 2014-01-22
HEFEI QIANXIN BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this medium is that the cost of raw materials is high due to the content of lactose and peptone

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Add 9kg of corn flour (fineness of 100 mesh, the same below), 1.5kg of soybean meal powder (fineness of 80 mesh, the same below) and 0.75kg of dipotassium hydrogen phosphate into the container, and dilute to 0.75 m 3 , after stirring evenly, sterilize at 121°C for 25 minutes, cool to 40°C, add 5wt% (ie 10% of the weight of the seed liquid) into the seed liquid, and ferment for 40 hours. Fermentation conditions: The fermentation temperature is 28-32°C, the aeration ratio is 1:0.8, and the rotation speed is 200-250 rpm.

[0029] The residual sugar (determined by anthrone-sulfuric acid method) was 4% of that before inoculation, the bacterial concentration (determined by plate dilution method, three times in parallel) was an average of 2.99 billion CFU / ml, and the sporulation rate was about 90%.

Embodiment 2

[0031] Add 10.5kg of corn flour, 1.1kg of soybean flour and 0.9kg of dipotassium hydrogen phosphate into the container, and dilute to 0.75m with water 3 , after stirring evenly, sterilize at 121°C for 25 minutes, cool to 35°C, add seed liquid at 5wt%, and ferment for 36h. Fermentation conditions: fermentation temperature is 28-32°C, ventilation ratio is 1:0.8, rotation speed is 0-8h, 150-200 rpm; 9-28h, 280-320 rpm; 29-36h, 200-350 rpm / Minute.

[0032] Residual sugar (determined by anthrone-sulfuric acid method) was 3% of that before inoculation, the bacterial concentration (determined by plate dilution method, three times in parallel) was an average of 4.16 billion CFU / ml, and the sporulation rate was about 95%.

Embodiment 3

[0034] Add 14kg of corn flour, 1.5kg of soybean meal and 1.5kg of dipotassium hydrogen phosphate into the container, and dilute to 0.75m with water 3 , after stirring evenly, sterilize at 121°C for 25 minutes, cool to 25°C, add seed solution at 5wt%, and ferment for 38h. Fermentation conditions: The fermentation temperature is 28-32°C, the aeration ratio is 1:0.8, and the rotation speed is 200-250 rpm.

[0035] The residual sugar (determined by anthrone-sulfuric acid method) was 3% of that before inoculation, the bacterial concentration (determined by plate dilution method, three times in parallel) was an average of 1.96 billion CFU / ml, and the sporulation rate was about 90%.

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Abstract

The invention belongs to the field of microbial culture technology, relates to a process technology adopting a bacillus mucilaginosus industrial liquid to form spores, and particularly relates to a submerged fermentation culture medium of bacillus mucilaginosus applied to microbial fertilizers. The culture medium comprises the following raw materials according to the weight percentage: 0.9-1.5% of corn flour, 0.1-0.3% of soybean meal, 0.08-0.2% of dipotassium phosphate, and the balance being water. The culture medium is obtained by the steps of mixing the corn flour, the soybean meal, dipotassium phosphate and water, stirring evenly, sterilizing for 20-40 min at the temperature of 121 DEG C, and then cooling to 25-40 DEG C to obtain the culture medium. The pH value of the culture medium is in the range of about 6.0-7.5, and the spores of bacillus mucilaginosus grow well in the pH value of 6.0-8.0, so the culture medium can promote thalli growth and spore formation without using an acid or alkali to adjust pH before sterilization, simplifies operation, improves the product quality, reduces the production cost, and is more suitable for industrial mass production.

Description

[0001] technical field [0002] The invention belongs to the technical field of microorganism culture, and relates to a process technology for forming spores of bacillus coliformis in an industrialized liquid, in particular to a submerged fermentation medium for bacillus colloidis used in microbial fertilizers. Background technique [0003] Bacillus colioids can increase the content of soluble phosphorus and potassium in the soil, and can also promote crop growth and enhance crop disease resistance, so it is widely used in microbial fertilizers. [0004] Because spores have strong stress resistance and are not easy to die, the number of spores of effective strains in microbial fertilizers is an important factor affecting the shelf life. During the cultivation of Bacillus colloidus, polysaccharides are easily produced, and polysaccharides will increase the viscosity of the fermentation broth, thereby inhibiting the growth of bacteria, and then affecting the number of Bacillus...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/07
Inventor 钱执初杨柳朱奎峰郭粹锦
Owner HEFEI QIANXIN BIOTECH DEV
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