A preparation method of a microcapsule of gastric acid-resistant bacillus licheniformis with high survival rates

A technology of Bacillus licheniformis and Bacillus licheniformis, applied in the field of preparation of probiotic microcapsules, can solve the problems of low survival rate and poor gastric acid resistance

Active Publication Date: 2015-10-14
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through the interaction between polysaccharides and polyelectrolytes with opposite charges, or between polyelectrolytes and small molecule cross-linking agents, polyelectrolyte membranes are formed to design probiotic microcarriers, such as chitosan-alginic acid microcapsules , calcium alginate gel microspheres, etc., there have been many research reports, but there are still many deficiencies, such as: poor gastric acid resistance, low survival rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation method of the gastroacid-resistant bacillus licheniformis microcapsules that the present embodiment has high survival rate may further comprise the steps:

[0029] (1) Strain activation: Inoculate the thawed wild-type B. licheniformis IBL 331 bacterial liquid preserved in glycerin at about -80°C in MRS liquid medium, and culture it at 37°C and 200rpm for 15 hours to inoculate the bacteria. Kind of activation, to obtain activated seed liquid;

[0030] (2) Expanded cultivation: Inoculate the activated seed liquid in an Erlenmeyer flask equipped with fresh MRS liquid medium with pH 6.8 at an inoculum size of 4%, and cultivate it for 4 hours at 37°C and 200rpm to obtain high-concentration licheniform spores Bacillus culture;

[0031] (3) Preparation of bacterial suspension: Centrifuge the culture of Bacillus licheniformis at 4°C and 4000 rpm for 30 minutes, remove the supernatant, and obtain the precipitated bacteria; add distilled water, Shake and mix wel...

Embodiment 2

[0038] The preparation method of the gastroacid-resistant bacillus licheniformis microcapsules that the present embodiment has high survival rate may further comprise the steps:

[0039] (1) Strain activation: Inoculate the thawed wild-type B. licheniformis IBL 331 bacterial liquid preserved in glycerin at about -80°C in MRS liquid medium, and culture it at 39°C and 240rpm for 15 hours to inoculate the bacteria. Kind of activation, to obtain activated seed liquid;

[0040] (2) Expansion cultivation: inoculate the activated seed liquid in an Erlenmeyer flask equipped with fresh MRS liquid medium with pH 7.2 at an inoculum size of 6%, and cultivate it for 4 hours at 39°C and 240rpm to obtain high-concentration licheniformis spores Bacillus culture;

[0041] (3) Preparation of bacterial suspension: centrifuge the culture of Bacillus licheniformis at 4°C and 6000 rpm for 20 minutes, remove the supernatant, and obtain the precipitated bacteria; add physiological saline to the prec...

Embodiment 3

[0048] The preparation method of the present embodiment bacillus licheniformis microcapsules, comprises the following steps:

[0049] (1) Strain activation: Inoculate the thawed wild-type B. licheniformis IBL 331 bacterial liquid preserved in glycerin at about -80°C in MRS liquid medium, and culture it at 37°C and 200rpm for 15 hours to inoculate the bacteria. Kind of activation, to obtain activated seed liquid;

[0050] (2) Expanded cultivation: Inoculate the activated seed liquid in an Erlenmeyer flask equipped with fresh MRS liquid medium with pH 6.8 at an inoculum size of 4%, and cultivate it for 4 hours at 37°C and 200rpm to obtain high-concentration licheniform spores Bacillus culture;

[0051] (3) Preparation of bacterial suspension: Centrifuge the culture of Bacillus licheniformis at 4°C and 4000 rpm for 30 minutes, remove the supernatant, and obtain the precipitated bacteria; add distilled water, Shake and mix well; centrifuge to remove the supernatant under the sam...

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Abstract

The present invention belongs to the technical field of preparation of probiotic microcapsules and particularly relates to a preparation method of a microcapsule of gastric acid-resistant bacillus licheniformis with high survival rates. The preparation method conducts an optimal control of the temperature, pH and culture medium during the culture expansion process, and the prepared polyelectrolyte microcapsule has a honeycomb core and an outer shell-like structure, and is preserved combining drying and vacuum-packaging. The produced bacillus licheniformis microcapsules are placed in a simulated gastric fluid, thus the bacillus licheniformis has high survival rates and high environmental tolerance. The product not only can be applied in the field of health-care food, but also can be applied in the field of medicine. The bacterial strains are probiotics and the carrier materials are natural polysaccharide macromolecules, thus the microcapsules are green, safe and environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of preparation of probiotic microcapsules, in particular to a method for preparing gastric acid-resistant bacillus licheniformis microcapsules with high survival rate. Background technique [0002] Bacillus licheniformis (Bacillus licheniformis) is a bacterium of the family Bacillus, which is a Gram-positive bacillus. Bacillus licheniformis can regulate the micro-ecological balance among the intestinal microbial flora, between the microbial flora and the host. Diseases caused by intestinal microecological dysfunction, such as bacterial or viral diarrhea, acute and chronic enteritis, can be adjusted by taking Bacillus licheniformis preparations to adjust the intestinal flora imbalance, so as to achieve the purpose of treating and restoring intestinal function; at the same time, lichen Bacillus can prompt the body to produce antibacterial active substances to kill pathogenic bacteria. In addition, Bacillus l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/30A23P1/04A61K35/742A61K9/50A61K47/36A61P1/12A61P1/00C12N1/20C12R1/10
CPCA61K9/5036A61K35/742C12N1/20
Inventor 吴庆喜童望宇谢球
Owner ANHUI UNIVERSITY
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