Composite preparation for improving culture survival rate of procambarus clarkii and application method thereof

A technology of Procambarus clarkii and survival rate, which is applied in the field of preparation of compound biological preparations, can solve problems such as poor application effect, failure of breeding, neglect of overall environmental regulation methods, etc., to improve oxidation-reduction potential, maintain long-term stability, and maintain The effect of high-quality bottom and water environment

Active Publication Date: 2019-09-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the biological agents in the field of Procambarus clarkii adjust the breeding environment from the perspective of "individual microorganisms", and the products are mostly single or compound str

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Application case of Procambarus clarkii cement pond culture. The present embodiment includes component A and component B, wherein component A is 25% of Bacillus subtilis, 25% of Bacillus coagulans, 25% of Pseudomonas, and 25% of Marinobacterium dehydrocarbon. Component B is vanillin 18%, acetyltransferase 17%, activated carbon powder 15%, polyacrylamide 20%, sodium lauryl sulfate 15%, calcium peroxide 15%. The dosage of component B is 15mg / m 3 Water body; the dosage of component A after expansion is 5 mL / m 3 Water body.

[0031] Table one is the effect that this embodiment is applied to Procambarus clarkii cement pond cultivation:

[0032] Table 1. Application effect of compound preparation cement pool

[0033] project value NH before administration 4 + -N (mg / L)

[0034] Remarks: a, the breeding process was 120 days, and the compound preparation was used 3 times, with an average interval of 40 days; b, except for the compound preparation, oth...

Embodiment 2

[0036] Application case of Procambarus clarkii cultured in earthen ponds. This embodiment includes component A and component B, wherein component A is 15% of Bacillus subtilis, 17% of Bacillus coagulans, 33% of Pseudomonas, and 35% of Marinobacter aldohydrocarbons. Component B is vanillin 12%, acetyltransferase 13%, sodium nitrate 10%, activated carbon powder 10%, calcium hydroxide 5%, polyacrylamide 20%, sodium lauryl sulfate 15%, sodium percarbonate 15% %. The dosage of component B is 5 mg / m 3 Water body; the dosage of component A after expansion is 3 mL / m 3 Water body.

[0037] Table two is the effect that this embodiment is applied to Procambarus clarkii earthen pond culture:

[0038] Table 2. Application effect of compound preparation earth pond

[0039] project value NH before administration 4 + -N (mg / L)

[0040] Remarks: a, the breeding process was 147 days, and the compound preparation was used 3 times, with an average interval of 50 days; ...

Embodiment 3

[0042]Application case of Procambarus clarkii in paddy fields. This embodiment includes component A and component B, wherein component A is 20% of Bacillus subtilis, 30% of Bacillus coagulans, 25% of Pseudomonas, and 25% of Marinobacterium dehydrocarbon. Component B is vanillin 5%, acetyltransferase 5%, sodium nitrate 10%, activated carbon powder 10%, calcium hydroxide 10%, polyacrylamide 30%, sodium lauryl sulfate 15%, sodium percarbonate 15% %. The dosage of component B is 7 mg / m 3 Water body; the dosage of component A after expansion is 4 mL / m 3 Water body.

[0043] Table three is the effect that this embodiment is applied to Procambarus clarkii paddy field cultivation:

[0044] Table 3. Application effect of compound preparation in paddy field culture

[0045] project value NH before administration 4 + -N (mg / L)

[0046] Remarks: a, the breeding process was 160 days, and the compound preparation was used 3 times, with an average interval of 50 d...

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Abstract

The invention discloses a composite preparation for improving the culture survival rate of procambarus clarkii and an application method thereof. The composite preparation is mainly prepared from a component A and a component B, wherein the component A is prepared from 30-50% of bacillus and 50%-70% of aerobic denitrifying bacteria; the component B is prepared from 10%-15% of a quorum sensing quencher, 10%-15% of a synergist, 20%-30% of a flocculant, 10%-15% of a surfactant and 20%-30% of an oxygenating agent. The component B of the preparation is used firstly, and meanwhile the component A is used later after culture expansion. The preparation can improve the oxidative reduction potential at the bottom of a culture environment, reduce the propagation of cyanobacteria and microcystis aeruginosa in a culture water body, and decrease harmful flora in the bottom environment and harmful algae in the water body based on the principles of quorum sensing quenching, microbial ecological occupation, microbial matrix competition, microbial metabolite inhibition and the like by applying the component A and the component B in a synergistic sequence, so that the production of toxic substances such as microcystins, hydrogen sulfide, ammonia nitrogen and nitrite is basically avoided, and thus the survival rate of cultured procambarus clarkii is improved. The preparation has the advantages of quick action, strong effect sustainability and the like.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and specifically relates to a compound biological agent preparation technology and a use method for improving the survival rate of Procambarus clarkii culture by improving the culture bottom and water body environment. Background technique [0002] Procambarus clarkii ( Procambarus clarkia ), commonly known as crayfish, is a product that is deeply loved by consumers. At present, it has become an important species in rice field farming and pond farming in my country, and it also provides effective support for ensuring the safety of vegetable baskets. [0003] However, due to the large amount of high-protein feed fed during the breeding process, it is very easy to cause toxic ammonia nitrogen, hydrogen sulfide, nitrite and other substances in the bottom environment. At the same time, the residual nutrient elements are in the water body, and the high temperature season will easily lead to the o...

Claims

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Application Information

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IPC IPC(8): A01K61/59C12N1/20C02F3/34C12R1/125C12R1/07C12R1/38C12R1/01C02F103/20
CPCA01K61/59C02F3/34C02F3/348C02F2103/20C02F2303/04C02F2305/04C12N1/20Y02A40/81
Inventor 阮贇杰徐向阳孔德栋殷旭旺蔡磊陆慧锋周凡丁雪燕
Owner ZHEJIANG UNIV
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