CIK cell culture method
A cell culture and cell technology, applied in the direction of cell culture active agent, culture process, tissue culture, etc., can solve the problems of poor activity and proliferation ability of CIK cells, to improve activity and proliferation ability, adequate nutrition and good environment, shorten cycle effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0041] In this embodiment, a CIK cell culture method comprises the following steps:
[0042] (1) Resuspend peripheral blood mononuclear cells in CIK medium to obtain cell suspension;
[0043] (2) Add the cell suspension to the T175 culture flask that has been pre-coated with anti-CD3 monoclonal antibody and retronectin, and then place it at 37°C, 5% CO 2 In a saturated humidity incubator; adjust the cell density to 7×10 before culturing 5-2×10 6 / mL;
[0044] (3) On the third day of culture, 60 mL of CIK culture medium was supplemented;
[0045] (4) On the 6th day of culture, after adding the CIK culture solution, pour the original culture solution in the original T175 culture bottle into a new culture bottle, blow off the cells on the wall of the original T175 culture bottle with the culture solution, and pour Put into a new culture bottle, add CIK culture solution to a total volume of 400mL, divide the above culture solution into four culture bags, add CIK culture solut...
Embodiment 2
[0064] In this embodiment, a CIK cell culture method comprises the following steps:
[0065] (1) Resuspend peripheral blood mononuclear cells in CIK medium to obtain cell suspension;
[0066] (2) Add the cell suspension to the T175 culture flask that has been pre-coated with anti-CD3 monoclonal antibody and retronectin, and then place it at 37°C, 5% CO 2 In a saturated humidity incubator; adjust the cell density to 7×10 before culturing 5 -1.5×10 6 / mL;
[0067] (3) On the third day of culture, 60 mL of CIK culture medium was supplemented;
[0068] (4) On the 6th day of culture, after adding the CIK culture solution, pour the original culture solution in the original T175 culture bottle into a new culture bottle, blow off the cells on the wall of the original T175 culture bottle with the culture solution, and pour Put into a new culture bottle, add CIK culture solution to a total volume of 400mL, divide the above culture solution into four culture bags, add CIK culture so...
Embodiment 3
[0088] In this embodiment, a CIK cell culture method comprises the following steps:
[0089] (1) Resuspend peripheral blood mononuclear cells in CIK medium to obtain cell suspension;
[0090] (2) Add the cell suspension to the T175 culture flask that has been pre-coated with anti-CD3 monoclonal antibody and retronectin, and then place it at 37°C, 5% CO 2 In a saturated humidity incubator; adjust the cell density to 7×10 before culturing 5 -1.5×10 6 / mL;
[0091] (3) On the third day of culture, 60 mL of CIK culture medium was supplemented;
[0092] (4) On the 6th day of culture, after adding the CIK culture solution, pour the original culture solution in the original T175 culture bottle into a new culture bottle, blow off the cells on the wall of the original T175 culture bottle with the culture solution, and pour Put into a new culture bottle, add CIK culture solution to a total volume of 400mL, divide the above culture solution into four culture bags, add CIK culture solu...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap