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Preparation method of semi-solid micro-ecological preparation for aquaculture

A micro-ecological preparation and aquaculture technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of inconvenient transportation, long storage period, and limited application range

Active Publication Date: 2018-01-12
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The water dosage form is easy to use and has good effect, but has a short storage period and is inconvenient to transport and carry; the powder dosage form is small in size and has a long storage period, but it needs to be activated before use, and the effect is unstable, etc.
And whether it is liquid or powder, its application range has certain limitations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: a kind of semi-solid microbial preparation and preparation method thereof for aquaculture, adopt following processing steps:

[0032] (1) Cultivation of Bacillus natto: Take a ring of Bacillus natto from the inclined surface of the test tube ( Basillus natto , CICC10454, provided by China Industrial Microorganism Culture Collection Management Center) bacteria lawn, inoculated into 50mL sterilized broth medium, at 30 ℃, 120 r min -1 Incubate on a shaker for 24 hours. Then put it into 1L of sterilized broth medium, at 30 °C, 120 r min -1 Cultivate on a shaker for 36 hours, and prepare the strain seed solution for later use. At this time, the number of viable bacteria in the bacterial solution is ≥10 9 CFU·mL -1 .

[0033] The broth liquid medium, containing peptone 10 g L -1 , beef extract 5 g·L -1 , NaCl 5 g·L -1 , adjust the pH to about 7.0, sterilize at 121°C for 20 minutes, and cool down for later use.

[0034] (2) Cultivation of Lactobacillus...

Embodiment 2

[0045] Embodiment 2: a kind of semi-solid microbial preparation and preparation method thereof for aquaculture, adopt following processing steps:

[0046] (1) Cultivation of Bacillus natto: Take a ring of Bacillus natto from the inclined surface of the test tube ( Basillus natto , CICC10454, provided by the China Industrial Microbiology Culture Collection Management Center) bacterial lawn, inoculated into 50mL sterilized broth medium, at 32°C, 125r min -1 Incubate on a shaker for 22 hours. Then put it into 1L of sterilized broth medium, at 32°C, 125r min -1 Cultivate on a shaker for 36 hours, and prepare the strain seed solution for later use. At this time, the number of viable bacteria in the bacterial solution is ≥10 9 CFU·mL -1 .

[0047] The broth liquid culture medium contains peptone 12g L -1 , beef extract 6g·L -1 , NaCl 5g·L -1 , adjust the pH to about 7.0, sterilize at 121°C for 20 minutes, and cool down for later use.

[0048] (2) Cultivation of Lactobacil...

Embodiment 3

[0059] Embodiment 3: a kind of semi-solid microbial preparation and preparation method thereof for aquaculture, adopt following processing steps:

[0060] (1) Cultivation of Bacillus natto: Take a ring of Bacillus natto from the inclined surface of the test tube ( Basillus natto , CICC10454, provided by the China Industrial Microbiology Culture Collection Management Center) bacterial lawn, inoculated into 60mL sterilized broth medium, at 30°C, 122r min -1 Incubate for 25 hours on a shaker. Then put it into 1L of sterilized broth medium, at 30 ℃, 122r min -1 Cultivate on a shaker for 38 hours, and prepare the strain seed solution for later use. At this time, the number of viable bacteria in the bacterial solution is ≥10 9 CFU·mL -1 .

[0061] The broth liquid medium, containing peptone 9g L -1 , beef extract 6g·L -1 , NaCl 5g·L -1 , adjust the pH to about 7.0, sterilize at 121°C for 20 minutes, and cool down for later use.

[0062] (2) Cultivation of Lactobacillus: T...

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Abstract

The invention relates to a preparation method of a semi-solid micro-ecological preparation for aquaculture and belongs to the technical field of the aquaculture. The preparation method includes that byproduct bran, wheat middling and soy sauce residues of industrial and agricultural production are taken as a fermentation matrix, microbiological strains of bacillus natto CICC 10454, lactobacillus acidophilus CICC 21007, acetobacter aceti CICC 21684, saccharomyces cerevisiae CICC 1005 and candida utilis which have different characters and ecological activities are used for mixed fermentation toform a fermentation product high in viable count, protein and amino acid content, and the semi-solid micro-ecological preparation integrated with functions of water purification, pathogenic inhibition, intestinal repairing, liver protection, digestion promotion, disease prevention and growth promotion and the like is obtained via vacuum package. Characters of water and powder of the microbial preparation are combined with those of multi-strains effectively, so that the semi-solid micro-ecological preparation is long in storage life, high in activity and convenient to carry; the semi-solid micro-ecological preparation can be used directly by being sprayed or stirred with materials as well as used after culture expansion.

Description

technical field [0001] The invention relates to a preparation method of a semi-solid micro-ecological preparation for aquaculture, belonging to the technical field of aquaculture. Background technique [0002] Since 1988, China's aquaculture production has ranked first in the world for 28 consecutive years, becoming the world's largest aquaculture country. At present, the main aquaculture mode in China is high-density pond culture, that is, a culture mode in which high-density seedlings are placed and a large amount of fertilization and feeding are used to obtain high yields. Although the high-density breeding method has increased the breeding output per unit water body, it has caused the deterioration of the breeding water environment, which has caused a series of corresponding problems. Nitrogen and phosphorus in the aquaculture water environment have increased sharply, serious organic pollution, decreased transparency, deteriorating water quality, and serious bottom poll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/16A23K10/18C12R1/07C12R1/225C12R1/02C12R1/865C12R1/72
Inventor 吴伟潘莹郑尧陈树桥周国勤
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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