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Fishery ocean microbial ecological preparation and preparation method thereof

A micro-ecological preparation, marine technology, applied in the field of micro-ecological preparations to achieve the effect of promoting popularization and application and improving feed efficiency

Inactive Publication Date: 2015-09-09
NOC XIAMEN BIO TEC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, Lactobacillus plantarum and Pediococcus pentosaceus are usually used as microbial additives for aquatic organisms respectively, and there are few reports on the comprehensive use of the two

Method used

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  • Fishery ocean microbial ecological preparation and preparation method thereof
  • Fishery ocean microbial ecological preparation and preparation method thereof
  • Fishery ocean microbial ecological preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A marine micro-ecological preparation for fishery, comprising Lactobacillus plantarum and Pediococcus pentosaceae, the bacteria number ratio of Lactobacillus plantarum and Pediococcus pentosaceae is 2:1.

[0031] The fermented liquid from this ratio experiment was used for a pilot breeding test. After 10 days of feeding, the shrimp in the experimental group were significantly larger than those in the other groups, with thicker intestines, strong peristaltic ability, and fast feeding. Therefore, the optimal fermentation ratio of Lactobacillus plantarum and Pediococcus pentosaceus is 2:1.

[0032] We fed lactic acid bacteria to 0.7cm juvenile shrimp, and compared the groups that were not fed, the effect was particularly obvious. The juvenile shrimp that were fed according to the ratio of 2:1 showed strong vitality, obvious bouncing response when frightened, The intestinal tract is relatively thick, the peristalsis ability is strong, and the tail fan has a large expansion ...

Embodiment 2

[0034] A preparation method of marine probiotics for fishery, comprising the following steps:

[0035] Step 1. Activation and expansion of strains:

[0036] Primary culture: Take a slant from the ultra-clean workbench and save a single colony in 100ml of sterilized MRS medium, and place it on a constant temperature water bath shaker at 35°C for 24 hours; inoculate 4 bottles of Lactobacillus plantarum and Pediococcus pentosacea each, in order to activate Bacterial strains: after 24 hours, take the bacterial liquid and spread it on several MRS plates, place it in a 35°C incubator and culture it for 24 hours, wait for the colony to grow, and put it in a 4°C refrigerator to preserve the strain and wait for use;

[0037] Secondary culture: Inoculate a single colony from a slanted surface in an ultra-clean workbench into 200ml sterilized MRS, inoculate a total of 6 bottles of each of the two bacteria, and culture on a shaker in a constant temperature water bath at 35°C for 24 hours;...

Embodiment 3

[0045] Example 3 Study on the concentration ratio of Lactobacillus plantarum and Pediococcus pentosaceus

[0046] The ratio of Lactobacillus plantarum to Pediococcus pentosacea is designed to be: 1:1, 1:2, 1:3, 1:4, 2:1, 3:1 six ratios. Proportionally inoculated into MRS medium (sterilized at 121°C for 30 minutes), placed in a 35°C constant temperature water bath incubator for 12 hours with slow shaking, and the bacterial liquid was taken to detect the number of bacteria and pH, and each ratio was paralleled in triplicate. The test results were based on mean value, the results are shown in Table 1 and figure 1 shown.

[0047] Table 1 Effect of the ratio of two lactic acid bacteria on their metabolites

[0048] Lactobacillus plantarum / Pediococcus pentosacea 1:1 1:2 1:3 2:1 3:1 Test 1 group 5.2 4.7 3.8 3.9 3.7 Test 2 groups 5.1 4.3 4.4 3.4 3.8 Test 3 groups 4.7 4.5 3.8 3.2 3.5 PH (average) 5 4.5 4 3.5 3.7 Test 1 gro...

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Abstract

The invention discloses a fishery ocean microbial ecological preparation. The preparation comprises lactobacillus pantarum and pediococcus pentosaceus, wherein the microbial content ratio of lactobacillus pantarum to pediococcus pentosaceus is 2:1. The invention further discloses a preparation method of the fishery ocean microbial ecological preparation. The preparation method comprises a first step of activation and culture expansion of strains; a second step of preparation of a fermentation medium; a third step of fermentation cultivation; a fourth step of filtering and material discharging. A culture medium formula facilitating large-scale production is screened out for lactobacillus pantarum and pediococcus pentosaceus, the feed efficiency is greatly improved, metabolites of lactobacillus pantarum and pediococcus pentosaceus are synergistic, the efficiency of the metabolites of lactobacillus pantarum and pediococcus pentosaceus is much higher than that of a metabolite of lactobacillus pantarum or pediococcus pentosaceus in production application, and popularization and application of lactobacillus pantarum and pediococcus pentosaceus microbial ecological preparation for aquaculture are effectively promoted.

Description

technical field [0001] The invention relates to the technical field of microecological preparations, in particular to a marine microecological preparation for fishery and a preparation method thereof. Background technique [0002] At present, there are more and more serious diseases in the aquaculture industry. Traditional antibiotics and chemical disinfectants are unsustainable. The resulting discharge of aquaculture water has brought serious pollution to the aquaculture area. Since the 1990s, photosynthetic bacteria have been used in aquaculture. The application technology continues to improve. In recent years, the application of probiotics in aquaculture is in the ascendant. The technology of bacillus in the aquaculture industry is becoming more and more mature, which provides a reference for the application of microorganisms in the aquaculture industry. China Taiwan 9 Lactic acid bacteria were used in the breeding industry in the 10s, and have a certain level of applicatio...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/25C12R1/01
CPCC12N1/20
Inventor 肖潮雄陈学豪熊玲芳陈启春陈朝文
Owner NOC XIAMEN BIO TEC CO LTD
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