A cultivation method for and application of antrodiacamphorata mycelia

A kind of technology of Antrodia cintrodia mycelium and cultivation method, which is applied in the cultivation of Antrodia cinnamomea mycelium and its application field, can solve the problems of Antrodia cintrodia in short supply, short cycle, slow growth of Antrodia cintrodia in cinnamomea sawdust, etc., to improve health care function, easy Controlling, enhancing the effect of efficacy

Inactive Publication Date: 2018-06-15
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In recent years, the Antrodia camphorata industry has developed rapidly, not only in Taiwan, but also in various parts of the mainland. However, Antrodia camphorata grows very slowly on the camphor wood chips, which cannot meet market demand. After a lot of manpower, material and financial resources, Great breakthroughs and developments have been made in the artificial cultivation of Antrodia cinnamomea, but the current market for Antrodia cinnamomea is still in short supply, so t...

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  • A cultivation method for and application of antrodiacamphorata mycelia
  • A cultivation method for and application of antrodiacamphorata mycelia
  • A cultivation method for and application of antrodiacamphorata mycelia

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Example 1: Antrodia antrodia was inoculated on the slant medium, and the components of the slant medium were 18% potato, 2% glucose, 1.5% peptone, 3% lactose, 3% yeast powder, 0.6% vitamin B1, 5% agar, 0.15 %K 2 HPO 4 , 0.1% MgSO 4 , and the balance is water. Cultivate in the dark at 17°C for 7 days, wait until the slope is covered with mycelia of Antrodia cinnamomea, inoculate the strains of Antrodia cinnamomea into the culture medium of shake flasks, and perform primary shake flask culture to obtain the first-grade species of production shake flasks. The culture conditions are: The volume ratio of flask medium to shake flask is 200:1000mL / mL, and the components of shake flask medium are 18% potato, 2% glucose, 1.5% peptone, 3% lactose, 3% yeast powder, 0.6% vitamin B1, 0.15%K 2 HPO 4 , 0.1% MgSO 4 , the balance is water, insert 1.5cm 2 The strains cultured on the slant were cultured by shaking at 150rpm for 7 days at a temperature of 20°C; the strains of Antrod...

Embodiment 2

[0049] Embodiment 2: Antrodia antrodia is inoculated on the slant medium, and the slant medium components are 20% potato, 2.5% glucose, 2% peptone, 4% lactose, 4% yeast powder, 0.8% vitamin B1, 7% agar, 0.2 %K 2 HPO 4 , 0.2% MgSO 4 , and the balance is water. Cultivate in the dark at 22°C for 8 days, wait until the slope is covered with mycelia of Antrodia cinnamomea, inoculate the strains of Antrodia cinnamomea into the liquid medium, and carry out primary shake flask culture to obtain the primary species of production shake flask, the culture condition is shake flask The volume ratio of culture medium to shake flask is 200:1000mL / mL, insert 1.5cm 2 The strains cultured on the slant, the shake flask medium components are 20% potato, 2.5% glucose, 2% peptone, 4% lactose, 4% yeast powder, 0.8% vitamin B1, 0.2% K 2 HPO 4 , 0.2% MgSO 4 , and the balance is water. Shaking culture at 150rpm for 7 days, temperature 30°C; inoculate Antrodia camphorata fungus and shake flask cu...

Embodiment 3

[0050] Example 3: Antrodia antrodia was inoculated on the slant medium, and the components of the slant medium were 19% potato, 2% glucose, 2% peptone, 2.5% lactose, 3.5% yeast powder, 0.7% vitamin B1, 6% agar, 0.15 %K 2 HPO 4 , 0.1% MgSO 4 , and the balance is water. Cultivate in the dark at 17-22°C for 7 days, wait until the slant is covered with mycelia of Antrodia cinnamomea, inoculate the strains of Antrodia cinnamomea into the culture medium of shake flasks, and carry out primary shake flask culture to obtain the first-grade species of production shake flasks, culture conditions For the volume ratio of shake flask culture medium to shake flask is 200:1000mL / mL, insert 1.5cm 2 The strains cultured on the slant, the shake flask medium components are 19% potato, 2% glucose, 2% peptone, 3.5% lactose, 3.5% yeast powder, 0.7% vitamin B1, 0.15% K 2 HPO 4 , 0.1% MgSO 4 , the balance is water, shake and cultivate at 150rpm for 7 days, and the temperature is 30°C; the Antrod...

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Abstract

The invention provides a cultivation method for and application of antrodiacamphorata mycelia. The cultivation method for antrodiacamphorata mycelia comprises the steps of: (1) culture expansion in shake flasks: inoculating antrodia camphorate strains to an inclined surface culture substrate for inclined surface culture, inoculating the antrodia camphorate strains to a liquid culture substrate after the inclined surface culture and performing primary shake flask culture, and then inoculating the antrodia camphorate strains to a liquid culture substrate for secondary shake flask culture; (2) culture expansion in seeding tanks: inoculating production shake flask second-level seeds into a fermentation culture substrate for culture expansion to obtain first-level antrodia camphorate strains; (3) culture expansion in fermentation tanks: inoculating the first-level antrodia camphorate strains into fermentation tanks with fermentation culture substrates for fermentation tank culture, and performing filtering to obtain antrodiacamphorata mycelia. Antrodiacamphorata mycelia produced by using the method have the advantages of short production period and high quality. Beverages prepared fromthe antrodiacamphorata mycelia have a good health care effect.

Description

technical field [0001] The invention relates to a method for cultivating mycelium and its application, in particular to a method for cultivating mycelia of Antrodia camphorata and its application. Background technique [0002] Antrodia camphorata (Antrodia camphorata), also known as Antrodia camphorata, Camphor fungus, and Cinnamomum cinnamomea, is a rare medicinal species of Basidiomycotina, Phytomycetes, Phyllobacteria, Polyporacea, and Phyllostachys genus. fungus. Earlier, it was used by the aborigines of Taiwan, China to treat liver disease caused by long-term drinking, and was known as "the ruby ​​in the forest" and "the king of medicine". [0003] Antrodia Cinnamomea is a unique medicinal fungus in Taiwan, which is extremely precious. Wild Antrodia Cinnamomea is only born in the mountains between 450-2000m above sea level in Taiwan, China, in the inner wall of the decayed heartwood of the camphor tree trunk, or on the wet surface of the dead and fallen camphor tree wo...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/20A23L2/38A23L33/00A23L2/02C12N1/14C12R1/645
CPCA23L2/02A23L2/382A23L33/00C12N1/14A23V2002/00A23V2250/628A23V2250/5072A23V2250/5086A23V2250/032A23V2200/30
Inventor 曹正谢春芹许俊齐凡军民宋振潇
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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